Vincenzo L'Imperio, Giulia Capitoli, Giorgio Cazzaniga, Mauro Mannino, Francesca Bono, Davide Seminati, Catarina Eloy, Joao Pinto, Elena Guerini Rocco, Matteo Fassan, Pasquale Pisapia, Francesco Pepe, Lara Pijuan, Jordi Temprana-Salvador, Antonio Polonia, Syed Ali Khurram, Emanuela Bonoldi, Alessandro Marando, Giuseppe Perrone, Stefania Galimberti, Giancarlo Troncone, Umberto Malapelle, Fabio Pagni
{"title":"分子病理学中肿瘤细胞分数定量的数字工具的常规使用：量子的国际验证。","authors":"Vincenzo L'Imperio, Giulia Capitoli, Giorgio Cazzaniga, Mauro Mannino, Francesca Bono, Davide Seminati, Catarina Eloy, Joao Pinto, Elena Guerini Rocco, Matteo Fassan, Pasquale Pisapia, Francesco Pepe, Lara Pijuan, Jordi Temprana-Salvador, Antonio Polonia, Syed Ali Khurram, Emanuela Bonoldi, Alessandro Marando, Giuseppe Perrone, Stefania Galimberti, Giancarlo Troncone, Umberto Malapelle, Fabio Pagni","doi":"10.32074/1591-951X-1100","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>The absolute and relative quantification of tumor cell fraction (TCF) in tissue samples for molecular pathology testing is time-consuming and poorly reproducible.</p><p><strong>Methods: </strong>Here we report the results of an international survey on non-small cell lung cancer (NSCLC), validating the Qupath Analysis of Nuclei from Tumor to Uniform Molecular tests (QuANTUM) automated computational pipeline for TCF quantification.</p><p><strong>Results: </strong>The TCF obtained with QuANTUM is reliable, as demonstrated by the comparison with the manual counting of cells (ground truth, GT) in cell blocks, small biopsies and surgical specimens (overall correlation of 0.89). The visual evaluation of QuANTUM-processed images increased the pathologists' agreement with GT and QuANTUM of +0.16, +0.21, +0.09 and +0.17, +0.29, +0.21 across the three sample types, respectively. An overall increase in cases classified as containing ≥100 tumor cells for all sample types was noted after QuANTUM (from 75 cases, 63% to 96 cases, 80% among cell blocks, p = 0.003).</p><p><strong>Conclusions: </strong>QuANTUM is an easy-to-use and reliable tool for the TCF assessment and its employment significantly modifies the visual estimation by pathologists, improving the assessment of NSCLC cases for molecular analysis.</p>","PeriodicalId":45893,"journal":{"name":"PATHOLOGICA","volume":"117 3","pages":"269-277"},"PeriodicalIF":4.4000,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12236135/pdf/","citationCount":"0","resultStr":"{\"title\":\"The routine use of a digital tool for the tumor cell fraction quantification in molecular pathology: an international validation of QuANTUM.\",\"authors\":\"Vincenzo L'Imperio, Giulia Capitoli, Giorgio Cazzaniga, Mauro Mannino, Francesca Bono, Davide Seminati, Catarina Eloy, Joao Pinto, Elena Guerini Rocco, Matteo Fassan, Pasquale Pisapia, Francesco Pepe, Lara Pijuan, Jordi Temprana-Salvador, Antonio Polonia, Syed Ali Khurram, Emanuela Bonoldi, Alessandro Marando, Giuseppe Perrone, Stefania Galimberti, Giancarlo Troncone, Umberto Malapelle, Fabio Pagni\",\"doi\":\"10.32074/1591-951X-1100\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>The absolute and relative quantification of tumor cell fraction (TCF) in tissue samples for molecular pathology testing is time-consuming and poorly reproducible.</p><p><strong>Methods: </strong>Here we report the results of an international survey on non-small cell lung cancer (NSCLC), validating the Qupath Analysis of Nuclei from Tumor to Uniform Molecular tests (QuANTUM) automated computational pipeline for TCF quantification.</p><p><strong>Results: </strong>The TCF obtained with QuANTUM is reliable, as demonstrated by the comparison with the manual counting of cells (ground truth, GT) in cell blocks, small biopsies and surgical specimens (overall correlation of 0.89). The visual evaluation of QuANTUM-processed images increased the pathologists' agreement with GT and QuANTUM of +0.16, +0.21, +0.09 and +0.17, +0.29, +0.21 across the three sample types, respectively. An overall increase in cases classified as containing ≥100 tumor cells for all sample types was noted after QuANTUM (from 75 cases, 63% to 96 cases, 80% among cell blocks, p = 0.003).</p><p><strong>Conclusions: </strong>QuANTUM is an easy-to-use and reliable tool for the TCF assessment and its employment significantly modifies the visual estimation by pathologists, improving the assessment of NSCLC cases for molecular analysis.</p>\",\"PeriodicalId\":45893,\"journal\":{\"name\":\"PATHOLOGICA\",\"volume\":\"117 3\",\"pages\":\"269-277\"},\"PeriodicalIF\":4.4000,\"publicationDate\":\"2025-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12236135/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"PATHOLOGICA\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.32074/1591-951X-1100\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PATHOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"PATHOLOGICA","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.32074/1591-951X-1100","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PATHOLOGY","Score":null,"Total":0}
The routine use of a digital tool for the tumor cell fraction quantification in molecular pathology: an international validation of QuANTUM.
Objective: The absolute and relative quantification of tumor cell fraction (TCF) in tissue samples for molecular pathology testing is time-consuming and poorly reproducible.
Methods: Here we report the results of an international survey on non-small cell lung cancer (NSCLC), validating the Qupath Analysis of Nuclei from Tumor to Uniform Molecular tests (QuANTUM) automated computational pipeline for TCF quantification.
Results: The TCF obtained with QuANTUM is reliable, as demonstrated by the comparison with the manual counting of cells (ground truth, GT) in cell blocks, small biopsies and surgical specimens (overall correlation of 0.89). The visual evaluation of QuANTUM-processed images increased the pathologists' agreement with GT and QuANTUM of +0.16, +0.21, +0.09 and +0.17, +0.29, +0.21 across the three sample types, respectively. An overall increase in cases classified as containing ≥100 tumor cells for all sample types was noted after QuANTUM (from 75 cases, 63% to 96 cases, 80% among cell blocks, p = 0.003).
Conclusions: QuANTUM is an easy-to-use and reliable tool for the TCF assessment and its employment significantly modifies the visual estimation by pathologists, improving the assessment of NSCLC cases for molecular analysis.
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