物理化学,微生物和微生物组动态在酒厂废物堆肥。

IF 5.8 3区 环境科学与生态学 0 ENVIRONMENTAL SCIENCES
Gregoria Mitropoulou, Ioanna Karapantzou, Ioanna Prapa, Dimitra Papanikolaou, Vasileios Charovas, Yiannis Kourkoutas
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引用次数: 0

摘要

与被广泛研究的食物垃圾或动物粪便等堆肥基质相比,酿酒厂的废物带来了独特的挑战和机遇。其高含量的木质素、纤维素和多酚化合物需要特定的微生物联合体来有效降解,如果不适当平衡,可能会潜在地抑制微生物活动。在本研究中,将传统的微生物计数与高分辨率微生物组分析相结合,对酒庄废弃物堆肥进行了分析,这是一种很少应用于此类农工残渣的方法。此外,还进行了一项实际的概念验证研究,将堆肥产品作为葡萄栽培中部分基质的替代品,关闭葡萄园废物管理的循环。关键参数,如水分含量、pH值、温度、电导率和C/N比,在60天的时间内进行监测,以及酶活性的变化和微生物种群的变化,表明微生物活动的动态。在处理结束时,观察到碳氮比(C/N)降低,pH稳定到中性值,脱氢酶活性明显下降。微生物组分析显示8个细菌门和6个真菌门。鉴定出酸杆菌门、armatimonadees、拟杆菌门、Candidatus Saccharibacteria、Chloroflexi、蓝藻门、plantomycetes。同时还检出子囊菌门、担子菌门、壶菌门、昆虫菌门、肾小球菌门和毛霉门。该堆肥没有植物毒性,并且支持葡萄藤的生长,与商业基质相当。通过物化稳定性、缺乏植物毒性和促进葡萄生长的有效性,证明了酒庄废物微生物堆肥可以稳定地生产生物肥料,这表明堆肥作为一种可持续的废物管理解决方案在酿酒业中具有潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Physicochemical, microbial, and microbiome dynamics in winery waste composting.

Compared to more extensively studied composting substrates like food waste or animal manure, winery waste presents unique challenges and opportunities. Its high content of lignin, cellulose, and polyphenolic compounds demands specific microbial consortia for efficient degradation and can potentially inhibit microbial activity if not properly balanced. In the present study, analysis of winery waste composting that combines traditional microbial enumeration with high-resolution microbiome profiling, an approach rarely applied to this type of agro-industrial residue, was implemented. Moreover, a practical proof-of-concept study, for using the composted product as a partial substrate replacement in grapevine cultivation, closing the loop in vineyard waste management, was conducted. Key parameters, such as moisture content, pH, temperature, conductivity, and C/N ratio were monitored, over a 60-day period, along with changes in enzymatic activity and shifts in microbial populations, indicating dynamic microbial activity. At the end of the process, a reduction in the carbon-to-nitrogen (C/N) ratio was observed, pH was stabilized to neutral values, and dehydrogenases activity was notably decreased. Microbiome analysis revealed eight bacterial and six fungal phyla. Acidobacteria, Armatimonadetes, Bacteroidetes, Candidatus Saccharibacteria, Chloroflexi, Cyanobacteria, Planctomycetes were identified. The Ascomycota, Basidiomycota, Chytridiomycota, Entomophthoromycota, Glomeromycota, and Mucoromycota fungal phyla were also detected. The compost exhibited no phytotoxicity and supported grapevine growth comparable to commercial substrates. Winery waste microbial composting led to stable biofertilizer production, evidenced by physicochemical stability, lack of phytotoxicity, and effectiveness in promoting grapevine growth suggesting the potential of composting as a sustainable waste management solution in the winemaking industry.

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来源期刊
CiteScore
8.70
自引率
17.20%
发文量
6549
审稿时长
3.8 months
期刊介绍: Environmental Science and Pollution Research (ESPR) serves the international community in all areas of Environmental Science and related subjects with emphasis on chemical compounds. This includes: - Terrestrial Biology and Ecology - Aquatic Biology and Ecology - Atmospheric Chemistry - Environmental Microbiology/Biobased Energy Sources - Phytoremediation and Ecosystem Restoration - Environmental Analyses and Monitoring - Assessment of Risks and Interactions of Pollutants in the Environment - Conservation Biology and Sustainable Agriculture - Impact of Chemicals/Pollutants on Human and Animal Health It reports from a broad interdisciplinary outlook.
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