Jinyoung Kim , Jong-Woo Lim , Hyun-Ouk Kim , Joowon Park , Eunji Jeong , Chaewon Park , Geunseon Park , Minjoo Yeom , Daesub Song , Eun-Kyung Lim , Seungjoo Haam
{"title":"基于血凝素对接分析的乌米诺韦抗病毒治疗流感病毒的目视检测","authors":"Jinyoung Kim , Jong-Woo Lim , Hyun-Ouk Kim , Joowon Park , Eunji Jeong , Chaewon Park , Geunseon Park , Minjoo Yeom , Daesub Song , Eun-Kyung Lim , Seungjoo Haam","doi":"10.1016/j.bios.2025.117762","DOIUrl":null,"url":null,"abstract":"<div><div>The rapid evolution of viruses poses a significant threat to global health, as evidenced by recent pandemics. Umifenovir (Arbidol®), a broad-spectrum antiviral drug, has demonstrated potential in treating various viral infections. This study aimed to assess the antiviral mechanism of umifenovir against influenza A virus (IAV), specifically its interaction with hemagglutinin (HA), using umifenovir-modified gold nanoparticles (umi-GNPs). Umi-GNPs have been applied to various IAV subtypes, including H1N1, H3N2, H4N6, H6N8, and H9N2. Our results demonstrate that umifenovir effectively inhibits viral replication in all tested subtypes at a viral titer of 10<sup>4.5</sup> 50 % egg infective dose (EID<sub>50</sub>)/mL. Additionally, the aggregation of umi-GNPs with viruses pre-bound to umifenovir was suppressed, indicating that umi-GNPs bind to a specific region of the viral protein. These findings indicate that umi-GNPs can serve as novel agents for visually determining umifenovir resistance in IAV.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"287 ","pages":"Article 117762"},"PeriodicalIF":10.7000,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Hemagglutinin docking analysis-based visual detection of antiviral therapeutic efficacy of umifenovir against influenza virus\",\"authors\":\"Jinyoung Kim , Jong-Woo Lim , Hyun-Ouk Kim , Joowon Park , Eunji Jeong , Chaewon Park , Geunseon Park , Minjoo Yeom , Daesub Song , Eun-Kyung Lim , Seungjoo Haam\",\"doi\":\"10.1016/j.bios.2025.117762\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The rapid evolution of viruses poses a significant threat to global health, as evidenced by recent pandemics. Umifenovir (Arbidol®), a broad-spectrum antiviral drug, has demonstrated potential in treating various viral infections. This study aimed to assess the antiviral mechanism of umifenovir against influenza A virus (IAV), specifically its interaction with hemagglutinin (HA), using umifenovir-modified gold nanoparticles (umi-GNPs). Umi-GNPs have been applied to various IAV subtypes, including H1N1, H3N2, H4N6, H6N8, and H9N2. Our results demonstrate that umifenovir effectively inhibits viral replication in all tested subtypes at a viral titer of 10<sup>4.5</sup> 50 % egg infective dose (EID<sub>50</sub>)/mL. Additionally, the aggregation of umi-GNPs with viruses pre-bound to umifenovir was suppressed, indicating that umi-GNPs bind to a specific region of the viral protein. These findings indicate that umi-GNPs can serve as novel agents for visually determining umifenovir resistance in IAV.</div></div>\",\"PeriodicalId\":259,\"journal\":{\"name\":\"Biosensors and Bioelectronics\",\"volume\":\"287 \",\"pages\":\"Article 117762\"},\"PeriodicalIF\":10.7000,\"publicationDate\":\"2025-07-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biosensors and Bioelectronics\",\"FirstCategoryId\":\"1\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0956566325006384\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOPHYSICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biosensors and Bioelectronics","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0956566325006384","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOPHYSICS","Score":null,"Total":0}
Hemagglutinin docking analysis-based visual detection of antiviral therapeutic efficacy of umifenovir against influenza virus
The rapid evolution of viruses poses a significant threat to global health, as evidenced by recent pandemics. Umifenovir (Arbidol®), a broad-spectrum antiviral drug, has demonstrated potential in treating various viral infections. This study aimed to assess the antiviral mechanism of umifenovir against influenza A virus (IAV), specifically its interaction with hemagglutinin (HA), using umifenovir-modified gold nanoparticles (umi-GNPs). Umi-GNPs have been applied to various IAV subtypes, including H1N1, H3N2, H4N6, H6N8, and H9N2. Our results demonstrate that umifenovir effectively inhibits viral replication in all tested subtypes at a viral titer of 104.5 50 % egg infective dose (EID50)/mL. Additionally, the aggregation of umi-GNPs with viruses pre-bound to umifenovir was suppressed, indicating that umi-GNPs bind to a specific region of the viral protein. These findings indicate that umi-GNPs can serve as novel agents for visually determining umifenovir resistance in IAV.
期刊介绍:
Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.