Dingyong Huang , Wanjia Tong , Jinfeng Wang , Jianjia Wang , Yan Zhuang , Peng Tian , Xiaolei Wang
{"title":"窦弧菌的全基因组序列,胶原降解的潜在贡献者","authors":"Dingyong Huang , Wanjia Tong , Jinfeng Wang , Jianjia Wang , Yan Zhuang , Peng Tian , Xiaolei Wang","doi":"10.1016/j.margen.2025.101201","DOIUrl":null,"url":null,"abstract":"<div><div>Collagenase is an enzyme that has been shown to be highly effective in the degradation of both native triple-helical collagen and its denatured form (<em>e.g.</em>, gelatin). As a virulence factor secreted by pathogenic bacteria (<em>Clostridium histolyticum</em>, <em>Vibrio</em>, <em>Bacillus cereus</em>), this efficacy is attributed to the unique ability of the enzyme to hydrolyze Gly-X-Y bonds within thermally stable fibrillar structures. A highly efficient gelatin degrading strain, <em>Vibrio sinus</em> S4M6<sup>T</sup>, was isolated from the surface seawater collected in Dongshan Bay (Fujian, PR China), but the key genes involved in gelatin degradation remain unknown. Here, we report the complete genome sequence of <em>Vibrio sinus</em> S4M6<sup>T</sup> and its collagen degrading genes. The genome of strain S4M6<sup>T</sup> consists of two circular chromosomes, with a total chromosome length of 4.78 Mbp and a GC content of 43.4 %. Genomic analysis revealed that strain S4M6<sup>T</sup> encodes a collagenase gene involved in collagen degradation. This study provides a genetic insight of collagen degradation in marine <em>Vibrio</em> species.</div></div>","PeriodicalId":18321,"journal":{"name":"Marine genomics","volume":"82 ","pages":"Article 101201"},"PeriodicalIF":1.5000,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Complete genome sequence of Vibrio sinus, a potential contributor to collagen degradation\",\"authors\":\"Dingyong Huang , Wanjia Tong , Jinfeng Wang , Jianjia Wang , Yan Zhuang , Peng Tian , Xiaolei Wang\",\"doi\":\"10.1016/j.margen.2025.101201\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Collagenase is an enzyme that has been shown to be highly effective in the degradation of both native triple-helical collagen and its denatured form (<em>e.g.</em>, gelatin). As a virulence factor secreted by pathogenic bacteria (<em>Clostridium histolyticum</em>, <em>Vibrio</em>, <em>Bacillus cereus</em>), this efficacy is attributed to the unique ability of the enzyme to hydrolyze Gly-X-Y bonds within thermally stable fibrillar structures. A highly efficient gelatin degrading strain, <em>Vibrio sinus</em> S4M6<sup>T</sup>, was isolated from the surface seawater collected in Dongshan Bay (Fujian, PR China), but the key genes involved in gelatin degradation remain unknown. Here, we report the complete genome sequence of <em>Vibrio sinus</em> S4M6<sup>T</sup> and its collagen degrading genes. The genome of strain S4M6<sup>T</sup> consists of two circular chromosomes, with a total chromosome length of 4.78 Mbp and a GC content of 43.4 %. Genomic analysis revealed that strain S4M6<sup>T</sup> encodes a collagenase gene involved in collagen degradation. This study provides a genetic insight of collagen degradation in marine <em>Vibrio</em> species.</div></div>\",\"PeriodicalId\":18321,\"journal\":{\"name\":\"Marine genomics\",\"volume\":\"82 \",\"pages\":\"Article 101201\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2025-07-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Marine genomics\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1874778725000376\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Marine genomics","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1874778725000376","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
Complete genome sequence of Vibrio sinus, a potential contributor to collagen degradation
Collagenase is an enzyme that has been shown to be highly effective in the degradation of both native triple-helical collagen and its denatured form (e.g., gelatin). As a virulence factor secreted by pathogenic bacteria (Clostridium histolyticum, Vibrio, Bacillus cereus), this efficacy is attributed to the unique ability of the enzyme to hydrolyze Gly-X-Y bonds within thermally stable fibrillar structures. A highly efficient gelatin degrading strain, Vibrio sinus S4M6T, was isolated from the surface seawater collected in Dongshan Bay (Fujian, PR China), but the key genes involved in gelatin degradation remain unknown. Here, we report the complete genome sequence of Vibrio sinus S4M6T and its collagen degrading genes. The genome of strain S4M6T consists of two circular chromosomes, with a total chromosome length of 4.78 Mbp and a GC content of 43.4 %. Genomic analysis revealed that strain S4M6T encodes a collagenase gene involved in collagen degradation. This study provides a genetic insight of collagen degradation in marine Vibrio species.
期刊介绍:
The journal publishes papers on all functional and evolutionary aspects of genes, chromatin, chromosomes and (meta)genomes of marine (and freshwater) organisms. It deals with new genome-enabled insights into the broader framework of environmental science. Topics within the scope of this journal include:
• Population genomics and ecology
• Evolutionary and developmental genomics
• Comparative genomics
• Metagenomics
• Environmental genomics
• Systems biology
More specific topics include: geographic and phylogenomic characterization of aquatic organisms, metabolic capacities and pathways of organisms and communities, biogeochemical cycles, genomics and integrative approaches applied to microbial ecology including (meta)transcriptomics and (meta)proteomics, tracking of infectious diseases, environmental stress, global climate change and ecosystem modelling.