促进腐假单胞菌木质素利用的两种新型绿色伯克霍尔德菌染料脱色过氧化物酶的特性研究

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products Pub Date : 2025-07-07 DOI:10.1016/j.indcrop.2025.121460

Feifan He, Runze Ma, Jie Zeng, Yuyan Zhang, Yongxuan Liu, Chunjie Gong, Xun Yu

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引用次数: 0

摘要

木质素增值在推进可持续生物炼制方面具有巨大潜力；然而，其结构的复杂性阻碍了其有效利用。在这项研究中，从洋葱伯克霍尔德菌ATCC 25416中鉴定并表征了两种新的染料脱色过氧化物酶（Dyps）， BcDyp1和BcDyp2。这两种酶具有较高的催化效率、广泛的pH适应性、良好的热稳定性和较强的底物抑制能力。为了促进木质素解聚，BcDyp1和BcDyp2在恶臭假单胞菌KT2440及其衍生物中异种表达和分泌，利用由强组成启动子P31驱动的修饰鞭毛III型分泌系统。重组菌株ΔfliC （pMB693-BcDyp1）和ΔfliCΔhcp （pMB693-BcDyp2）以木质素为唯一碳源培养时，其生长速度比对照菌株高3倍以上，表明其对木质素的利用能力有所提高。该研究扩展了具有理想工业性状的微生物Dyps的谱库，为木质素的利用提供了一条有前景的途径。

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Characterization of two novel dye-decolorizing peroxidases from Burkholderia cepacia for accelerating lignin utilization in Pseudomonas putida

Lignin valorization holds great potential for advancing sustainable biorefineries; however, its structural complexity hinders efficient utilization. In this study, two novel dye-decolorizing peroxidases (Dyps), BcDyp1 and BcDyp2, were identified and characterized from Burkholderia cepacia ATCC 25416. These two enzymes demonstrated high catalytic efficiency, broad pH adaptability, good thermostability, and strong resistance to substrate inhibition. To facilitate lignin depolymerization, BcDyp1 and BcDyp2 were heterologously expressed and secreted in Pseudomonas putida KT2440 and its derivatives using a modified flagellar type III secretion system driven by the strong constitutive promoter P31. The recombinant strains, ΔfliC (pMB693-BcDyp1) and ΔfliCΔhcp (pMB693-BcDyp2), exhibited over 3-fold higher growth rates compared to control strains when cultivated on lignin as the sole carbon source, highlighting their improved lignin utilization capabilities. This study expands the repertoires of microbial Dyps with desirable industrial traits, offering a promising approach for lignin utilization.

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来源期刊

Industrial Crops and Products 农林科学-农业工程

CiteScore

9.50

自引率

8.50%

发文量

1518

审稿时长

43 days

期刊介绍： Industrial Crops and Products is an International Journal publishing academic and industrial research on industrial (defined as non-food/non-feed) crops and products. Papers concern both crop-oriented and bio-based materials from crops-oriented research, and should be of interest to an international audience, hypothesis driven, and where comparisons are made statistics performed.