{"title":"利用EMS诱变和毒素筛选开发西瓜抗枯萎病种质资源的有效策略","authors":"Zhengjing Wu, Xinrong Fu, Qianjin Zhan, Zijing Lin, Weilin Zhu","doi":"10.1094/PDIS-12-24-2713-RE","DOIUrl":null,"url":null,"abstract":"<p><p>Fusarium wilt of watermelon (Citrullus lanatus), caused by Fusarium oxysporum f. sp. niveum, severely threatens watermelon production worldwide. This study, for the first time, explored a rapid and efficient method for creating disease-resistant watermelon germplasm by combining ethyl methanesulfonate (EMS) mutagenesis with crude toxin screening. Watermelon seeds treated with 1% EMS for 18 h achieved a germination rate of 52%, and resistant mutants were successfully identified using 50% crude toxin stock solution. The mutants exhibited enhanced stress tolerance, with significantly higher superoxide dismutase (SOD) and catalase (CAT) activities and lower malondialdehyde (MDA) content compared to susceptible varieties. Artificial fungal inoculation confirmed the resistance of mutant plants, which exhibited a disease index comparable to that of the resistant varieties. This study demonstrated the effectiveness of a practical and innovative strategy combining chemical mutagenesis and toxin screening for developing Fusarium wilt-resistant watermelon germplasm.</p>","PeriodicalId":20063,"journal":{"name":"Plant disease","volume":" ","pages":""},"PeriodicalIF":4.4000,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"An Efficient Strategy for Developing Fusarium Wilt-Resistant Watermelon Germplasm Using EMS Mutagenesis and Toxin Screening.\",\"authors\":\"Zhengjing Wu, Xinrong Fu, Qianjin Zhan, Zijing Lin, Weilin Zhu\",\"doi\":\"10.1094/PDIS-12-24-2713-RE\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Fusarium wilt of watermelon (Citrullus lanatus), caused by Fusarium oxysporum f. sp. niveum, severely threatens watermelon production worldwide. This study, for the first time, explored a rapid and efficient method for creating disease-resistant watermelon germplasm by combining ethyl methanesulfonate (EMS) mutagenesis with crude toxin screening. Watermelon seeds treated with 1% EMS for 18 h achieved a germination rate of 52%, and resistant mutants were successfully identified using 50% crude toxin stock solution. The mutants exhibited enhanced stress tolerance, with significantly higher superoxide dismutase (SOD) and catalase (CAT) activities and lower malondialdehyde (MDA) content compared to susceptible varieties. Artificial fungal inoculation confirmed the resistance of mutant plants, which exhibited a disease index comparable to that of the resistant varieties. This study demonstrated the effectiveness of a practical and innovative strategy combining chemical mutagenesis and toxin screening for developing Fusarium wilt-resistant watermelon germplasm.</p>\",\"PeriodicalId\":20063,\"journal\":{\"name\":\"Plant disease\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":4.4000,\"publicationDate\":\"2025-07-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant disease\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1094/PDIS-12-24-2713-RE\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant disease","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1094/PDIS-12-24-2713-RE","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
摘要
西瓜枯萎病是由尖孢镰刀菌（Fusarium oxysporum f. sp. niveum）引起的西瓜枯萎病，严重威胁着世界西瓜生产。本研究首次探索了甲基磺酸乙酯（EMS）诱变与粗毒素筛选相结合的快速高效西瓜抗病种质培育方法。用1%的EMS处理西瓜种子18 h，发芽率达到52%，用50%的粗毒素原液成功鉴定出抗性突变体。与易感品种相比，该突变体的超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性显著提高，丙二醛（MDA）含量显著降低。人工接种真菌证实了突变株的抗性，其病害指数与抗性品种相当。本研究证明了化学诱变与毒素筛选相结合的西瓜抗枯萎病种质资源开发策略的实用性和创新性。
An Efficient Strategy for Developing Fusarium Wilt-Resistant Watermelon Germplasm Using EMS Mutagenesis and Toxin Screening.
Fusarium wilt of watermelon (Citrullus lanatus), caused by Fusarium oxysporum f. sp. niveum, severely threatens watermelon production worldwide. This study, for the first time, explored a rapid and efficient method for creating disease-resistant watermelon germplasm by combining ethyl methanesulfonate (EMS) mutagenesis with crude toxin screening. Watermelon seeds treated with 1% EMS for 18 h achieved a germination rate of 52%, and resistant mutants were successfully identified using 50% crude toxin stock solution. The mutants exhibited enhanced stress tolerance, with significantly higher superoxide dismutase (SOD) and catalase (CAT) activities and lower malondialdehyde (MDA) content compared to susceptible varieties. Artificial fungal inoculation confirmed the resistance of mutant plants, which exhibited a disease index comparable to that of the resistant varieties. This study demonstrated the effectiveness of a practical and innovative strategy combining chemical mutagenesis and toxin screening for developing Fusarium wilt-resistant watermelon germplasm.
期刊介绍：
Plant Disease is the leading international journal for rapid reporting of research on new, emerging, and established plant diseases. The journal publishes papers that describe basic and applied research focusing on practical aspects of disease diagnosis, development, and management.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
