2型先天淋巴细胞在儿童变应性鼻炎腺样体肥大中的作用及相关的潜在治疗靶点

IF 4.2 2区 医学 Q2 IMMUNOLOGY
Journal of Inflammation Research Pub Date : 2025-07-01 eCollection Date: 2025-01-01 DOI:10.2147/JIR.S515707
Yan Hao, Tian-Yong Hu, Mei-Zhen Zhao, Xian-Hai Zeng, Ke Li, Bao-Hui Cheng, Da-Bo Liu
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引用次数: 0

摘要

目的:先天淋巴样细胞(ILC)是与免疫疾病相关的异质免疫细胞群。然而,它们在腺样体肥大(AH)、AH合并过敏性鼻炎(AH+AR)和AH合并中耳炎伴积液(AH+OME)中的具体作用仍知之甚少。本研究旨在表征这些疾病中ILC亚群及其与免疫学特征的关系。方法:采用流式细胞术定量测定AH、AH+AR或AH+OME患者腺样体组织中的ILC亚群,并进一步评估ILC亚群与临床、免疫学(血清和组织细胞因子)和组织病理学参数的相关性。结果:AH+AR组ST2 mRNA和蛋白表达明显高于AH和AH+OME组(p < 0.05)。AH+AR组血清IL-33高于AH组(p = 0.0127), IFN-γ高于AH+AR组(p = 0.0044)。AH和AH+AR组IL-4水平高于AH+OME组(p < 0.005)。流式细胞术显示,AH+AR中ILC2占主导地位(p = 0.0009 vs AH+OME), AH和AH+AR中ILC2/ILC1和ILC2/ILC3比值高于AH+OME (p < 0.05)。相关性分析显示,AH+AR患者ILC2与血清IgE、IL-4、IL-33、胸腺基质淋巴生成素(TSLP)及组织IL-4、IL-33、TSLP、IL-25呈正相关(p < 0.05)。ILC3与外周血嗜酸性粒细胞呈负相关(p = 0.0125),与血清和组织IL-17A呈正相关(p < 0.05)。结论:AH+AR患者腺样体组织中ILC2细胞显著富集,ST2和IL-33水平升高支持IL-33/ST2/ILC2信号通路的激活。靶向这一途径可能为AH合并变应性鼻炎提供新的治疗策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Role of Type 2 Innate Lymphoid Cells in Adenoid Hypertrophy with Allergic Rhinitis Among Children and Related Potential Therapeutic Targets.

Objective: Innate lymphoid cells (ILC) are a heterogeneous group of immune cells implicated in immune diseases. However, their specific roles in adenoid hypertrophy (AH), AH with allergic rhinitis (AH+AR), and AH with otitis media with effusion (AH+OME) remain poorly understood. This study aimed to characterize ILC subsets and their association with immunological profiles in these conditions.

Methods: Flow cytometry was used to quantify ILC subsets in adenoid tissues from patients with AH, AH+AR, or AH+OME, and correlations between ILC subsets and clinical, immunological (serum and tissue cytokines), and histopathological parameters were further assessed.

Results: ST2 mRNA and protein expression were significantly higher in AH+AR than in AH and AH+OME (p < 0.05). Serum IL-33 was elevated in AH+AR compared to AH (p = 0.0127), while IFN-γ was higher in AH than in AH+AR (p = 0.0044). IL-4 levels were higher in AH and AH+AR than in AH+OME (p < 0.005). Flow cytometry showed that ILC2 predominated in AH+AR (p = 0.0009 vs AH+OME), with higher ILC2/ILC1 and ILC2/ILC3 ratios in AH and AH+AR compared to AH+OME (p < 0.05). Correlation analysis indicated that ILC2 in AH+AR positively correlated with serum IgE, IL-4, IL-33, thymic stromal lymphopoietin (TSLP), and tissue IL-4, IL-33, TSLP, and IL-25 (p < 0.05). ILC3 inversely correlated with peripheral blood eosinophils (p = 0.0125) and positively with serum and tissue IL-17A (p < 0.05).

Conclusion: ILC2 cells are significantly enriched in adenoid tissues of patients with AH+AR, with elevated ST2 and IL-33 levels supporting the activation of the IL-33/ST2/ILC2 signaling pathway. Targeting this pathway may offer novel therapeutic strategies for AH combined with allergic rhinitis.

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来源期刊
Journal of Inflammation Research
Journal of Inflammation Research Immunology and Microbiology-Immunology
CiteScore
6.10
自引率
2.20%
发文量
658
审稿时长
16 weeks
期刊介绍: An international, peer-reviewed, open access, online journal that welcomes laboratory and clinical findings on the molecular basis, cell biology and pharmacology of inflammation.
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