设计一种基于细胞微rna的方法来沉默蝙蝠传播的尼帕病毒基因。

IF 1.9 4区 医学 Q3 NEUROSCIENCES
Nikita Kar, Supriyo Chakraborty
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引用次数: 0

摘要

蝙蝠传播的尼帕病毒以导致人类高死亡率而闻名,已在印度(Megaderma spasma)、孟加拉国(Pteropus medius)和马来西亚(Pteropus vampyrus)报告,不同种类的蝙蝠作为宿主。该病毒还感染各种动物,这些动物通常在传播给人类的过程中充当中间宿主。由于与尼帕病毒暴发相关的高死亡率,世界卫生组织已将其标记为一个重大的公共卫生问题,促使对抗病毒疗法和疫苗的开发进行广泛研究。然而,目前还没有有效的疫苗或治疗剂。在这种情况下,我们提出了一种基于mirna的方法来鉴定能够结合并可能切割或降解与人类感染有关的尼帕病毒基因的关键人类细胞mirna。我们的研究揭示了多种病毒基因中大量的miRNA结合位点,提示了基因沉默的潜在机制。此外,计算得到的自由能值(
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Designing a cellular MicroRNA-based approach to silence bat-borne Nipah virus genes.

The bat-borne Nipah virus, known for causing high mortality rates in humans, has been reported in India (Megaderma spasma), Bangladesh (Pteropus medius), and Malaysia (Pteropus vampyrus) with different bat species serving as reservoirs. The virus also infects various animals, which often act as intermediate hosts in the transmission to humans. Due to the high fatality rates associated with Nipah virus outbreaks, the World Health Organization has flagged it as a significant public health concern, prompting extensive research into the development of antiviral therapeutics and vaccines. However, no effective vaccine or therapeutic agent has yet been established. In this context, we propose a miRNA-based approach to identify key human cellular miRNAs capable of binding to and potentially cleaving or degrading Nipah virus genes implicated in human infections. Our study revealed a substantial number of miRNA binding sites across various viral genes, suggesting a potential mechanism for gene silencing. Furthermore, the calculated free energy values (< 4 kcal/mol) for all three regions; downstream, upstream and target indicate that the thermodynamically favorable binding could facilitate effective miRNA-mediated repressions of viral gene expression. Additionally, the translational efficiency and COSM values suggested swift miRNA-mediated cleavage or degradation of the viral genes. Moreover, analysis of the miRNA-mRNA duplex free energy and secondary structures, as predicted by RNAFold, indicated that the interactions between human miRNAs and Nipah virus genes were thermodynamically stable. These stable duplex formations support the potential for efficient binding, leading to effective gene silencing through cleavage or degradation mechanisms.

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来源期刊
Journal of NeuroVirology
Journal of NeuroVirology 医学-病毒学
CiteScore
6.60
自引率
3.10%
发文量
77
审稿时长
6-12 weeks
期刊介绍: The Journal of NeuroVirology (JNV) provides a unique platform for the publication of high-quality basic science and clinical studies on the molecular biology and pathogenesis of viral infections of the nervous system, and for reporting on the development of novel therapeutic strategies using neurotropic viral vectors. The Journal also emphasizes publication of non-viral infections that affect the central nervous system. The Journal publishes original research articles, reviews, case reports, coverage of various scientific meetings, along with supplements and special issues on selected subjects. The Journal is currently accepting submissions of original work from the following basic and clinical research areas: Aging & Neurodegeneration, Apoptosis, CNS Signal Transduction, Emerging CNS Infections, Molecular Virology, Neural-Immune Interaction, Novel Diagnostics, Novel Therapeutics, Stem Cell Biology, Transmissable Encephalopathies/Prion, Vaccine Development, Viral Genomics, Viral Neurooncology, Viral Neurochemistry, Viral Neuroimmunology, Viral Neuropharmacology.
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