微生理肾小球滤过障碍:当前的见解,创新和未来的应用。

IF 2.6 3区 生物学 Q3 MATERIALS SCIENCE, BIOMATERIALS
Manon Miran, Kieu Ngo, David Buob, Hanna Debiec, Pierre Ronco, Guillaume Perry
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引用次数: 0

摘要

慢性肾脏疾病(CKD)影响全球超过8.5亿人,通常进展到需要透析或肾脏移植的阶段。肾脏功能的核心是肾小球滤过屏障(GFB),它选择性地过滤废物,同时保留必需的蛋白质。传统的模型,包括动物研究和2D细胞培养,不能完全复制GFB的复杂性,限制了CKD的研究。微生理系统(MPS)的最新发展,特别是微生理肾小球滤过屏障(MPGFB),为研究肾脏疾病和评估治疗提供了更准确的体外模型。MPGFB系统使用器官芯片技术将足细胞和肾小球内皮细胞整合在受限的微流体环境中,密切模仿GFB的动态体内条件。这种设置可以进行详细的渗透性分析,帮助研究疾病机制和药物毒性。此外,在MPGFB平台中使用人类诱导的多能干细胞可以进行患者特异性研究,从而增强对遗传性肾脏疾病的了解。本文首先研究了GFB的结构和功能,重点介绍了其细胞和细胞外基质成分。然后讨论了MPGFB制造的生物和工程方法,涵盖材料,3D设计和流量控制。本文总结了MPGFB在疾病建模和药物测试中的应用,并提出了将MPGFB作为肾脏疾病研究和治疗的关键工具需要改进的地方。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Microphysiological Glomerular Filtration Barriers: Current Insights, Innovations, and Future Applications

Microphysiological Glomerular Filtration Barriers: Current Insights, Innovations, and Future Applications

Microphysiological Glomerular Filtration Barriers: Current Insights, Innovations, and Future Applications

Microphysiological Glomerular Filtration Barriers: Current Insights, Innovations, and Future Applications

Microphysiological Glomerular Filtration Barriers: Current Insights, Innovations, and Future Applications

Chronic kidney disease (CKD) affects over 850 million individuals worldwide, often progressing to stages requiring dialysis or kidney transplants. Central to kidney function is the glomerular filtration barrier (GFB), which selectively filters waste while retaining essential proteins. Traditional models, including animal studies and 2D cell cultures, fail to fully replicate the GFB's complexity, limiting CKD research. Recent developments in microphysiological systems (MPS), particularly microphysiological glomerular filtration barriers (MPGFB), provide more accurate in vitro models for studying kidney diseases and evaluating therapies. MPGFB systems use organ-on-chip technology to integrate podocytes and glomerular endothelial cells within confined microfluidic environments, closely mimicking GFB's dynamic in vivo conditions. This setup enables detailed permeability analysis, aiding in research on disease mechanisms and drug toxicity. Furthermore, using human-induced pluripotent stem cells in MPGFB platforms allows patient-specific studies, enhancing insights into genetic kidney disorders. This review first examines the GFB's structure and function, focusing on its cellular and extracellular matrix components. It then discusses biological and engineering approaches to MPGFB fabrication, covering materials, 3D design, and flow control. The review concludes with MPGFB applications in disease modeling and drug testing, and addresses improvements needed for refining MPGFB as a key tool in kidney disease research and treatment.

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来源期刊
Advanced biology
Advanced biology Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
6.60
自引率
0.00%
发文量
130
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