Haoyu Yang, Xunhao Wang, Jiayan Zhu, Yuzhou Liu, Gang Zhao, Jingyi Mi
{"title":"甜菜素通过下调Wnt/β-Catenin通路对类风湿关节炎的抗迁移、抗侵袭和抗炎作用","authors":"Haoyu Yang, Xunhao Wang, Jiayan Zhu, Yuzhou Liu, Gang Zhao, Jingyi Mi","doi":"10.1111/1756-185x.70360","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>This study investigates antimigratory, anti-invasive, and anti-inflammatory mechanisms of Bergenin in patients with rheumatoid arthritis (RA) by focusing on the pathways involved.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>In vitro, TNF-α-induced MH7A cells were used to assess Bergenin's effects on cell proliferation (CCK-8), migration (wound healing, Transwell assays), and apoptosis (flow cytometry). mRNA levels of IL-6 and MMPs were quantified by qRT-PCR, and protein expression was analyzed using ELISA and western blot. The Wnt/β-catenin pathway was examined through immunofluorescence and western blotting with or without LiCl. An adjuvant-induced arthritis (AIA) rat model was used to monitor clinical symptoms and analyze synovial tissue.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Bergenin inhibited the proliferation, migration, and invasion of TNF-α-induced MH7A cells, while promoting apoptosis. It reduced IL-6 and MMPs expression, with these effects reversed by LiCl. In vivo, Bergenin alleviated paw swelling, arthritis score, and synovial pathology in AIA rats, reducing pro-inflammatory factors.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>Bergenin exerts antimigratory, anti-invasive, and anti-inflammatory effects in rheumatoid arthritis by modulating the Wnt/β-catenin pathway, suggesting its potential as a therapeutic option for rheumatoid arthritis.</p>\n </section>\n </div>","PeriodicalId":14330,"journal":{"name":"International Journal of Rheumatic Diseases","volume":"28 7","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Antimigratory, Anti-Invasive and Anti-Inflammatory Effects of Bergenin in Rheumatoid Arthritis by Downregulating the Wnt/β-Catenin Pathway\",\"authors\":\"Haoyu Yang, Xunhao Wang, Jiayan Zhu, Yuzhou Liu, Gang Zhao, Jingyi Mi\",\"doi\":\"10.1111/1756-185x.70360\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p>This study investigates antimigratory, anti-invasive, and anti-inflammatory mechanisms of Bergenin in patients with rheumatoid arthritis (RA) by focusing on the pathways involved.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>In vitro, TNF-α-induced MH7A cells were used to assess Bergenin's effects on cell proliferation (CCK-8), migration (wound healing, Transwell assays), and apoptosis (flow cytometry). mRNA levels of IL-6 and MMPs were quantified by qRT-PCR, and protein expression was analyzed using ELISA and western blot. The Wnt/β-catenin pathway was examined through immunofluorescence and western blotting with or without LiCl. An adjuvant-induced arthritis (AIA) rat model was used to monitor clinical symptoms and analyze synovial tissue.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>Bergenin inhibited the proliferation, migration, and invasion of TNF-α-induced MH7A cells, while promoting apoptosis. It reduced IL-6 and MMPs expression, with these effects reversed by LiCl. 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Antimigratory, Anti-Invasive and Anti-Inflammatory Effects of Bergenin in Rheumatoid Arthritis by Downregulating the Wnt/β-Catenin Pathway
Background
This study investigates antimigratory, anti-invasive, and anti-inflammatory mechanisms of Bergenin in patients with rheumatoid arthritis (RA) by focusing on the pathways involved.
Methods
In vitro, TNF-α-induced MH7A cells were used to assess Bergenin's effects on cell proliferation (CCK-8), migration (wound healing, Transwell assays), and apoptosis (flow cytometry). mRNA levels of IL-6 and MMPs were quantified by qRT-PCR, and protein expression was analyzed using ELISA and western blot. The Wnt/β-catenin pathway was examined through immunofluorescence and western blotting with or without LiCl. An adjuvant-induced arthritis (AIA) rat model was used to monitor clinical symptoms and analyze synovial tissue.
Results
Bergenin inhibited the proliferation, migration, and invasion of TNF-α-induced MH7A cells, while promoting apoptosis. It reduced IL-6 and MMPs expression, with these effects reversed by LiCl. In vivo, Bergenin alleviated paw swelling, arthritis score, and synovial pathology in AIA rats, reducing pro-inflammatory factors.
Conclusions
Bergenin exerts antimigratory, anti-invasive, and anti-inflammatory effects in rheumatoid arthritis by modulating the Wnt/β-catenin pathway, suggesting its potential as a therapeutic option for rheumatoid arthritis.
期刊介绍：
The International Journal of Rheumatic Diseases (formerly APLAR Journal of Rheumatology) is the official journal of the Asia Pacific League of Associations for Rheumatology. The Journal accepts original articles on clinical or experimental research pertinent to the rheumatic diseases, work on connective tissue diseases and other immune and allergic disorders. The acceptance criteria for all papers are the quality and originality of the research and its significance to our readership. Except where otherwise stated, manuscripts are peer reviewed by two anonymous reviewers and the Editor.
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