大鼠慢性鼻窦炎诱导鼻窦微生物群继发性改变

IF 1.7 4区医学 Q2 OTORHINOLARYNGOLOGY

Laryngoscope Investigative Otolaryngology Pub Date : 2025-07-07 DOI:10.1002/lio2.70187

Fernanda Barriga-Chambi, Fabricio Ccami-Bernal, Frank Zela-Coila, Marial Hernandez-Caycho, Claudia Mares-Cuadros, Marcos Salas-Palma, Jorge Ballón-Echegaray, Renato Torres

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引用次数: 0

摘要

背景微生物群可能在慢性鼻窦炎（CRS）的炎症维持和延续中发挥作用。本研究旨在诱导大鼠的CRS，并与对照组比较微生物群组成。方法选用雄性Wistar大鼠16只。8只大鼠接受米曲霉蛋白酶和卵清蛋白的鼻内滴注，每周3次，连续12周（CRS组）。其余8人在相同条件下接受PBS治疗（对照组）。第13周，手术采集微生物样本，并对大鼠实施安乐死。对需氧菌、厌氧菌和真菌进行微生物培养，利用MALDI-TOF ms进行菌落鉴定，在门和属水平上分析相对丰度，利用Chao1指数评估物种丰富度。组织病理学分析评估粘膜、血管和上皮的变化，以确认慢性炎症。结果与对照组相比，CRS组的组织病理学改变更为频繁，尤其是粘膜改变(100%比25%；p = 0.01，卡方检验)。厚壁菌门在CRS大鼠中更丰富（60.3%比38.4%），这是由葡萄球菌增加（33.6%比77.7%）驱动的。CRS组的物种丰富度（85.0±23.3[61.7 ~ 108.3]）高于对照组（31.3±9.0 [22.2 ~ 40.3]）；p = 0.004；曼-惠特尼检验)。结论慢性炎症引起显著的菌群变化，CRS组菌群丰富度增加。这些发现表明，微生物群的变化可能是炎症环境的结果，而不是主要原因。证据水平基础科学。

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Induced Chronic Rhinosinusitis in Rats Leads to Secondary Changes in Sinonasal Microbiota

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Induced Chronic Rhinosinusitis in Rats Leads to Secondary Changes in Sinonasal Microbiota

Background

The microbiota may play a role in maintaining and perpetuating inflammation in chronic rhinosinusitis (CRS). This study aimed to induce CRS in rats and evaluate microbiota composition compared to controls.

Methods

Sixteen male Wistar rats were used. Eight rats received intranasal instillations of Aspergillus oryzae protease and ovalbumin three times weekly for 12 weeks (CRS group). The remaining eight received PBS (control group) under the same conditions. At week 13, microbiological samples were surgically collected, and the rats were euthanized. Microbiological cultures for aerobic, anaerobic bacteria and fungi were performed, and colonies were identified using MALDI-TOF MS. Relative abundance was analyzed at the phylum and genus levels, and species richness was assessed using the Chao1 index. Histopathological analysis evaluated mucosal, vascular, and epithelial changes to confirm chronic inflammation.

Results

Histopathological changes were more frequent in the CRS group compared to controls, especially mucosal changes (100% vs. 25%; p = 0.01, Chi-square test). Firmicutes were more abundant in CRS rats (60.3% vs. 38.4%), driven by an increase in Staphylococcus (33.6% vs. 77.7%). Species richness was higher in the CRS group (Chao1: 85.0 ± 23.3 [61.7–108.3]) compared to controls (31.3 ± 9.0 [22.2–40.3]; p = 0.004; Mann–Whitney U test).

Conclusions

Chronic inflammation induces significant microbiota changes, with an increase of species richness in the CRS group. These findings suggest microbiota shifts may result from inflammatory environments rather than being the primary cause.