Frontispiece: Whole-Cell Lysosome SMLM Imaging as Indicators for Functional Diagnostics with a Low-Phototoxic Spontaneously Blinking Probe

Aze-HMSiR, a near-infrared spontaneously blinking fluorophore, enables long-term SMLM imaging of lysosomes with minimal phototoxicity, as reported by Xiaogang Liu et al. in their Research Article (e202503177). Combining pH responsiveness and high photostability, it reveals critical lysosomal features—distribution, size, and lumen pH—under different anticancer treatments. This whole-cell imaging approach provides a powerful new tool for visualizing lysosomal dynamics and serves as a sensitive indicator for functional diagnostics in live-cell environments.