Jéssica Bezerra Maciel, Hortência Ribeiro Liberato, Antônio Wlisses da Silva, Maria Auxiliadora Solange Silva Gondim Pereira, Emanuela de Lima Rebouças, Francisco das Chagas L Pinto, Matheus Nunes da Rocha, Maria Kueirislene Amâncio Ferreira, Janaina Serra Azul Monteiro Evangelista, Izabel Florindo Guedes, Marcia Machado Marinho, Emmanuel Silva Marinho, Andreia Ferreira de Castro Gomes, Otília Loiola Pessoa, Jane Eire Silva Alencar de Menezes, Hélcio Silva Dos Santos
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引用次数: 0
摘要
摘要研究了从曼陀罗叶中分离得到的一种吲哚内酯——白藜芦醇B (Nicandrin B, Nic B)对成年斑马鱼(Danio rerio)的抗伤和抗炎作用。以Nic B(4、20和40 mg/kg)处理的动物无毒性,并保持正常的运动活动。该化合物可显著降低福尔马林和高渗生理盐水所致的伤害感觉;这些作用被TRPA1和瞬时受体电位香草样蛋白1拮抗剂逆转,表明这些靶点具有神经调节作用。此外,Nic B减轻了卡拉胶诱导的腹部水肿,减少了中性粒细胞的募集,降低了肝脏活性氧的水平。对接分析证实其与TRPA1具有良好的结合亲和力,支持其治疗潜力。这些发现表明,Nic B具有镇痛、抗炎活性和毒理学安全性,是一种有前景的候选药物。
Antinociceptive and Anti-inflammatory Effect of Nicandrin B Isolated From Datura ferox in Zebrafish.
Nicandrin B (Nic B), a withanolide isolated from Datura ferox leaves, was investigated for its antinociceptive and anti-inflammatory effects in adult zebrafish (Danio rerio). Animals treated with Nic B (4, 20, and 40 mg/kg) showed no toxicity and maintained normal locomotor activity. The compound significantly reduced nociception induced by formalin and hypertonic saline; these effects were reversed by TRPA1 and transient receptor potential vanilloid 1 antagonists, indicating neuromodulation of these targets. Additionally, Nic B attenuated carrageenan-induced abdominal edema, reduced neutrophil recruitment, and decreased hepatic reactive oxygen species levels. Docking analysis confirmed a favorable binding affinity with TRPA1, supporting its therapeutic potential. These findings suggest that Nic B combines analgesic, anti-inflammatory activity, and toxicological safety, making it a promising drug candidate.
期刊介绍:
Chemistry & Biodiversity serves as a high-quality publishing forum covering a wide range of biorelevant topics for a truly international audience. This journal publishes both field-specific and interdisciplinary contributions on all aspects of biologically relevant chemistry research in the form of full-length original papers, short communications, invited reviews, and commentaries. It covers all research fields straddling the border between the chemical and biological sciences, with the ultimate goal of broadening our understanding of how nature works at a molecular level.
Since 2017, Chemistry & Biodiversity is published in an online-only format.