基于DNA-SIP技术的三氯卡班在城市污泥厌氧消化中的降解行为及机理研究

IF 11.3 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Hazardous Materials Pub Date : 2025-07-05 DOI:10.1016/j.jhazmat.2025.139156

Xiaoye Tang , Mimi Zheng , Qianqian Zhang , Dechun He , Wangrong Liu , Yaxi Wang , Jiangguo Yang

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引用次数: 0

摘要

三氯卡班（TCC）普遍存在于城市污泥中，具有很高的生态健康风险。高级厌氧消化能有效去除污泥中的TCC，但其降解转化机理尚不清楚。为此，采用dna稳定同位素探测技术，研究了TCC在城市污泥厌氧消化过程中的降解行为及其机理。结果表明：TCC主要吸附在污泥相中，水相中TCC被有效去除（最大达95.2%）；水解和微生物转化对TCC的去除率有显著影响，微生物转化的贡献率为18.3 ~ 23.5%。经过高级厌氧消化后，通过水解、脱氯和羟基化形成了先前报道的四种产物（4,4 ' -二氯氨基苯胺（DCC）、二氯氨基苯胺（NCC）、4-氯苯胺和苯胺）和一种新产物（4,5-二氯-2-（甲胺）苯酚）。优势功能微生物（假单胞菌和拟杆菌）促进了TCC、DCC和NCC的降解。代谢途径在所有功能类别中占主导地位，尤其是碳水化合物和氨基酸代谢，分别占9.09%和7.59%。TCC水解功能基因tccA在DNA重片段中拷贝数较高。携带tccA基因的功能微生物优先利用¹³C，其他微生物优先利用¹²C。本研究对城市污泥深度厌氧消化过程中TCC的环境行为、命运和污染控制提供了新的认识。

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Degradation behavior and mechanism of triclocarban during municipal sludge anaerobic digestion: A study based on DNA-SIP technology

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Degradation behavior and mechanism of triclocarban during municipal sludge anaerobic digestion: A study based on DNA-SIP technology

Triclocarban (TCC) is universally present in municipal sludge, posing a high ecological health risk. Advanced anaerobic digestion can effectively remove TCC in sludge, but its degradation and transformation mechanism remains unclear. Thus, DNA-based stable isotope probing was conducted to investigate the degradation behavior and mechanism of TCC during municipal sludge anaerobic digestion. The results showed that TCC was mainly adsorbed in the sludge phase, and TCC in the aqueous phase was effectively removed (max 95.2 %). Hydrolysis and microbial transformation significantly influenced the removal of TCC, and the contribution of microbial transformation was 18.3–23.5 %. After advanced anaerobic digestion, four previously reported products (4,4’-dichlorocarbanilide (DCC), carbanilide (NCC), 4-chloroaniline, and aniline) and a new product (4,5-dichloro-2-(methylamino) phenol) were formed through hydrolysis, dechlorination, and hydroxylation. Dominant functional microorganisms (Pseudomonadota and Bacteroidota) enhanced the degradation of TCC, DCC, and NCC. Metabolic pathways dominated all functional categories, especially carbohydrate and amino acid metabolisms, accounting for 9.09 % and 7.59 %, respectively. TCC hydrolysis functional gene tccA had a high copy number in the heavy fraction of DNA. The functional microorganisms carrying the tccA gene preferentially utilized ¹ ³C, while others ¹ ²C utilized. This study provides a new insight into the environmental behavior, fate, and pollution control of TCC during the advanced anaerobic digestion of municipal sludge.

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来源期刊

Journal of Hazardous Materials 工程技术-工程：环境

CiteScore

25.40

自引率

5.90%

发文量

3059

审稿时长

58 days

期刊介绍： The Journal of Hazardous Materials serves as a global platform for promoting cutting-edge research in the field of Environmental Science and Engineering. Our publication features a wide range of articles, including full-length research papers, review articles, and perspectives, with the aim of enhancing our understanding of the dangers and risks associated with various materials concerning public health and the environment. It is important to note that the term "environmental contaminants" refers specifically to substances that pose hazardous effects through contamination, while excluding those that do not have such impacts on the environment or human health. Moreover, we emphasize the distinction between wastes and hazardous materials in order to provide further clarity on the scope of the journal. We have a keen interest in exploring specific compounds and microbial agents that have adverse effects on the environment.