短链氯化石蜡（C11, 64.2%Cl）对成年雄性斑马鱼的神经毒性和血脑屏障破坏作用

IF 7.3 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Pollution Pub Date : 2025-07-05 DOI:10.1016/j.envpol.2025.126782

Xinyu Liu , Jingui Liu , Shiqiang Dong , Chen Liu , Chao Wei , Jianan Ling , Ji Zheng , Xin Zheng

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引用次数: 0

摘要

短链氯化石蜡（SCCPs）是广泛分布于环境中的一系列持久性、生物蓄积性和毒性物质。然而，关于sccp神经毒性的信息很少。本研究以成年雄性斑马鱼（Danio rerio）为模型，分别暴露于0、50、250 μg/L的sccp中21 d，研究sccp的神经毒性作用。我们评估了SCCPs暴露21天后鱼类的游泳行为特征、乙酰胆碱酯酶（AChE）水平、神经炎症反应、紧密连接相关mRNA和蛋白水平以及血脑屏障（BBB）超微结构的变化。我们的研究表明，SCCPs显著削弱了斑马鱼的游泳能力和运动活性，激活了神经炎症反应，降低了鱼脑中AChE含量和活性，降低了紧密连接相关的mRNA和蛋白水平。此外，透射电镜（TEM）结果显示斑马鱼血脑屏障的超微结构变化和脑细胞凋亡，进一步证明慢性暴露于sccp可引起血脑屏障损伤。综上所述，本研究重点关注了斑马鱼慢性暴露于SCCPs，揭示了这种暴露可损害血脑屏障并导致神经损伤，并将为了解SCCPs的潜在健康风险提供重要见解，并强调了进一步研究的必要性。

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Short-chain chlorinated paraffins (C11, 64.2 %Cl) induced neurotoxicity and blood-brain barrier disruption in adult male zebrafish (Danio rerio)

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Short-chain chlorinated paraffins (C11, 64.2 %Cl) induced neurotoxicity and blood-brain barrier disruption in adult male zebrafish (Danio rerio)

Short-chain chlorinated paraffins (SCCPs) are a series of persistent, bioaccumulative and toxic (PBT) substances that are widely distributed in the environment. However, little information is available on SCCPs neurotoxicity. In present research, adult male zebrafish (Danio rerio) were used as a model and exposed to 0, 50, or 250 μg/L SCCPs for 21 days to investigate the neurotoxicity effect of SCCPs. We evaluated the swimming behavior traits, acetylcholinesterase (AChE) levels, neuroinflammatory response, tight junction-related mRNA and protein levels and blood-brain barrier (BBB) ultrastructural changes of fish following 21 days of SCCPs exposure. Our research showed that SCCPs significantly weakened swimming ability and locomotor activity of zebrafish, activated the neuroinflammatory response, decreased AChE content and activity, and the tight junction-related mRNA and protein levels in the fish brain. In addition, the results of transmission electron microscopy (TEM) revealed the ultrastructural changes of BBB and brain cell apoptosis in zebrafish, further proving that chronic exposure to SCCPs can cause BBB damage. In summary, this study focused on the chronic exposure of zebrafish to SCCPs, revealing that such exposure can damage the BBB and lead to nerve injury, and will provide important insights into the potential health risks associated with SCCPs and emphasize the necessity for further research.

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来源期刊

Environmental Pollution 环境科学-环境科学

CiteScore

16.00

自引率

6.70%

发文量

2082

审稿时长

2.9 months

期刊介绍： Environmental Pollution is an international peer-reviewed journal that publishes high-quality research papers and review articles covering all aspects of environmental pollution and its impacts on ecosystems and human health. Subject areas include, but are not limited to: • Sources and occurrences of pollutants that are clearly defined and measured in environmental compartments, food and food-related items, and human bodies; • Interlinks between contaminant exposure and biological, ecological, and human health effects, including those of climate change; • Contaminants of emerging concerns (including but not limited to antibiotic resistant microorganisms or genes, microplastics/nanoplastics, electronic wastes, light, and noise) and/or their biological, ecological, or human health effects; • Laboratory and field studies on the remediation/mitigation of environmental pollution via new techniques and with clear links to biological, ecological, or human health effects; • Modeling of pollution processes, patterns, or trends that is of clear environmental and/or human health interest; • New techniques that measure and examine environmental occurrences, transport, behavior, and effects of pollutants within the environment or the laboratory, provided that they can be clearly used to address problems within regional or global environmental compartments.