油橄榄曲霉内生菌对库蚊的生物杀虫活性:毒性、组织学和GC-MS分析。

IF 3.9 2区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
Amr H Hashem, Wesam M A Ward, Amer M Abdelaziz, Ahmed S Hashem, Mohamed A M Shahat
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引用次数: 0

摘要

内生真菌提供了一种可持续和环保的合成杀虫剂替代品,提供了有针对性的害虫控制,降低了环境毒性和抗性发展的风险。本研究从油橄榄中分离得到4种内生真菌,并对其乙酸乙酯提取物的杀蚊效果进行了研究。箱线图分布显示代谢物浓度与幼虫死亡率呈正相关,剂量越大,致死率越高。其中,提取物1和提取物2的杀虫活性最高,中位死亡率超过60%,而提取物3和提取物4的杀虫效果明显较弱。剂量响应曲线进一步证实了提取物1和2的效力,与提取物3和4相比,LC₅0值更低(148.36和153.36µg/mL)。组织学分析显示了剂量依赖性影响,25-50µg/mL时组织改变最小,而150-200µg/mL引起严重的组织紊乱、细胞凋亡和炎症反应。从形态学和分子结构上鉴定了最有效的分离株黑曲霉(Aspergillus niger,提取物1)和黄曲霉(Aspergillus flavus,提取物2),NCBI登录号分别为PQ269689和PQ269690。GC-MS分析鉴定出主要的生物活性成分,包括油酸和十六烷酸,支持其杀虫潜力。这些发现表明黑曲霉和黄曲霉乙酸乙酯提取物是一种很有前景的生物杀虫剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Bioinsecticidal activity of aspergillus-derived endophytes from Olea europaea against Culex pipiens: toxicity, histology, and GC-MS profiling.

Bioinsecticidal activity of aspergillus-derived endophytes from Olea europaea against Culex pipiens: toxicity, histology, and GC-MS profiling.

Bioinsecticidal activity of aspergillus-derived endophytes from Olea europaea against Culex pipiens: toxicity, histology, and GC-MS profiling.

Bioinsecticidal activity of aspergillus-derived endophytes from Olea europaea against Culex pipiens: toxicity, histology, and GC-MS profiling.

Endophytic fungi offer a sustainable and eco-friendly alternative to synthetic insecticides, providing targeted pest control with reduced environmental toxicity and lower risk of resistance development. In this study, four endophytic fungi were isolated from Olea europaea and their ethyl acetate extracts' larvicidal efficacy of Culex pipiens. Boxplot distributions revealed a positive correlation between metabolite concentration and larval mortality, with higher doses inducing greater lethality. Extracts 1 and 2 exhibited the highest larvicidal activity, with median mortality rates exceeding 60%, while Extracts 3 and 4 demonstrated significantly weaker effects. Dose-response curves further confirmed the potency of Extracts 1 and 2, with lower LC₅₀ values (148.36 and 153.36 µg/mL) compared to Extracts 3 and 4. The histological analysis revealed a dose-dependent impact, with minimal tissue alterations at 25-50 µg/mL, while 150-200 µg/mL caused severe disorganization, apoptosis, and inflammatory responses. The most potent isolates, Aspergillus niger(Extract 1) and Aspergillus flavus(Extract 2), were identified morphologically and molecularly, with NCBI accession numbers PQ269689 and PQ269690, respectively. GC-MS analysis identified key bioactive compounds, including oleic acid and hexadecanoic acid, supporting their insecticidal potential. These findings highlight A. niger and A. flavus ethyl acetate extracts as promising sources of bioinsecticides for pest control.

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来源期刊
Scientific Reports
Scientific Reports Natural Science Disciplines-
CiteScore
7.50
自引率
4.30%
发文量
19567
审稿时长
3.9 months
期刊介绍: We publish original research from all areas of the natural sciences, psychology, medicine and engineering. You can learn more about what we publish by browsing our specific scientific subject areas below or explore Scientific Reports by browsing all articles and collections. Scientific Reports has a 2-year impact factor: 4.380 (2021), and is the 6th most-cited journal in the world, with more than 540,000 citations in 2020 (Clarivate Analytics, 2021). •Engineering Engineering covers all aspects of engineering, technology, and applied science. It plays a crucial role in the development of technologies to address some of the world''s biggest challenges, helping to save lives and improve the way we live. •Physical sciences Physical sciences are those academic disciplines that aim to uncover the underlying laws of nature — often written in the language of mathematics. It is a collective term for areas of study including astronomy, chemistry, materials science and physics. •Earth and environmental sciences Earth and environmental sciences cover all aspects of Earth and planetary science and broadly encompass solid Earth processes, surface and atmospheric dynamics, Earth system history, climate and climate change, marine and freshwater systems, and ecology. It also considers the interactions between humans and these systems. •Biological sciences Biological sciences encompass all the divisions of natural sciences examining various aspects of vital processes. The concept includes anatomy, physiology, cell biology, biochemistry and biophysics, and covers all organisms from microorganisms, animals to plants. •Health sciences The health sciences study health, disease and healthcare. This field of study aims to develop knowledge, interventions and technology for use in healthcare to improve the treatment of patients.
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