大麻对蜱致损伤的治疗潜力:家兔生化和组织病理学评价。

Q2 Agricultural and Biological Sciences
Brazilian Journal of Biology Pub Date : 2025-06-30 eCollection Date: 2025-01-01 DOI:10.1590/1519-6984.294052
O Silva, D Sapatini, M L Rodrigues, B F Evangelista, B S Vicente, A S Marques, M L J Leitão, B C Lancia, M I Camargo-Mathias
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引用次数: 0

摘要

林奈鼻头蜱,俗称棕狗蜱,是狗的首选宿主,它们也会向狗传播各种病原体。控制这些体外寄生虫通常是通过使用合成化学产品来实现的,这可能导致耐药性的发展。从植物中提取的生物活性化合物正在被研究作为控制蜱虫的替代策略,包括大麻,它从其花中提取的提取物以其多种治疗特性而闻名,适用于人类和动物。因此,在本课题的初始工作中,研究了0.2、0.4和0.8 mg/mL三种油稀释度的苜蓿花提取物对林奈蜱宿主皮肤损伤的影响,以评估其愈合情况。此外,这一程序使得有必要评估,在本工作中所做的工作,这些动物的肝组织,作为提取物,虽然有杀蜱和促进皮肤损伤愈合的功能,也应该对宿主无害,被认为是可行的体外寄生虫控制。为此,应用组织学和组织化学技术对家兔肝脏进行组织学和组织化学处理(以确定形态学改变),将家兔肝脏分为:对照组(CG),暴露于1 mL油中,未见肝脏改变;处理1组(TG1),暴露于0.2 mg/mL,肝细胞出现细胞质空泡化;处理组2 (TG2),暴露于0.4 mg/mL,表现出强烈的形态学改变,包括肝细胞索紊乱、肝细胞肥大、细胞核改变和细胞死亡的迹象;处理组3 (TG3),暴露于0.8 mg/mL,也表现出组织紊乱和细胞空泡化,但程度低于TG2。除形态学评价外,还测定了肝酶AST和ALT的生化活性。TG2组ALT升高,其他组均无变化。总的来说,本研究得出结论,尽管不同稀释度的苜蓿花提取物具有杀蜱和愈合皮肤损伤(个人交流)的潜力,但它会对家兔造成不同程度的肝组织损伤,0.4 mg/mL稀释度显示出最高的肝毒性潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Therapeutic potential of Cannabis sativa against tick-induced injuries (Rhipicephalus linnaei): biochemical and histopathological evaluation in rabbits.

Rhipicephalus linnaei, commonly known as the brown dog tick, are preferential hosts for dogs, to whom they also transmit various pathogens. The control of these ectoparasites is generally achieved through the use of synthetic chemical products, which can lead to the development of resistance. Bioactive compounds extracted from plants are being studied as an alternative strategy for tick control, including Cannabis sativa, which provides an extract from its flowers known for its diverse therapeutic properties, applicable to both humans and animals. Therefore, in the work that originated this project investigated the effects of C. sativa flower extract in oil dilutions of 0.2, 0.4, and 0.8 mg/mL, applied to skin lesions of rabbit hosts of R. linnaei ticks, to evaluate their healing. In addition, this procedure made it necessary to evaluate, what was done in the present work, the hepatic tissue of these animals, as the extract, while functioning as a tickicide and promoting healing of skin lesions, should also be harmless to the hosts to be considered viable for ectoparasite control. For this purpose, histological and histochemical techniques were applied to the livers of the rabbits (to identify morphological alterations), which were divided into: control group (CG), exposed to 1 mL of oil, showing no hepatic alterations; treated group 1 (TG1), exposed to 0.2 mg/mL, showing cytoplasmic vacuolization in hepatocytes; treated group 2 (TG2), exposed to 0.4 mg/mL, displaying intense morphological alterations, including disorganization of hepatocyte cords, hepatocyte hypertrophy, altered nuclei, and signs of cell death; treated group 3 (TG3), exposed to 0.8 mg/mL, also showing tissue disorganization and cellular vacuolization, but to a lesser extent than TG2. In addition to morphological evaluation, the biochemical activity of hepatic enzymes AST and ALT was assessed. An increase in ALT was observed in TG2, while no other groups showed changes in these parameters. Overall, this study concluded that although the C. sativa flower extract at various dilutions has potential as a tickicide and for healing skin lesions (personal communication), it causes hepatic tissue damage in rabbits to varying degrees, with the 0.4 mg/mL dilution showing the highest hepatotoxic potential.

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来源期刊
CiteScore
2.40
自引率
0.00%
发文量
301
审稿时长
4-8 weeks
期刊介绍: The BJB – Brazilian Journal of Biology® is a scientific journal devoted to publishing original articles in all fields of the Biological Sciences, i.e., General Biology, Cell Biology, Evolution, Biological Oceanography, Taxonomy, Geographic Distribution, Limnology, Aquatic Biology, Botany, Zoology, Genetics, and Ecology. Priority is given to papers presenting results of researches in the Neotropical region. Material published includes research papers, review papers (upon approval of the Editorial Board), notes, book reviews, and comments.
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