{"title":"在DNBSEQ-G99RS平台上使用FGID法医四合一DNA分型试剂盒对170个STR和132个SNP标记进行平行测序。","authors":"Xiaoyuan Zhen, Zhenmin Zhao, Ruocheng Xia, Xiling Liu, Hui Li, Yuzhen Gao, Baifang He, Chengtao Li, Ruiyang Tao","doi":"10.1093/fsr/owae050","DOIUrl":null,"url":null,"abstract":"<p><p>Massive parallel sequencing (MPS) has rapidly emerged as a promising technique for forensic DNA typing due to its capacity to simultaneously detect numerous genetic markers and samples in a single reaction, allowing the direct acquisition of sequence information. In this current investigation, the FGID forensic four-in-one DNA typing kit was employed on the DNBSEQ-G99RS high-throughput sequencing platform to simultaneously analyse two types of forensic genetic markers-short tandem repeat (STR) and single nucleotide polymorphism (SNP). A total of 306 DNA markers, comprising Amelogenin, 66 autosomal STR (A-STR) loci, 29 X chromosomal STR (X-STR) loci, 75 Y chromosomal STR (Y-STR) loci, and 135 SNP (132 A-SNP and 3 Y-SNP) loci, were genotyped for 100 unrelated individual samples (50 males and 50 females). As a result, sequence-based STR typing identified 940 alleles on A-STRs, 378 alleles on X-STRs, and 519 alleles on Y-STRs. In comparison with length-based alleles, the number of unique alleles based on sequence increased by 58.18%. Additionally, 97 new sequence variations were observed at 29 STR loci, and MPS sequence information was obtained for the first time at 42 STR loci. Furthermore, when utilizing sequence-based data, forensic parameters exhibited a notable increase in combined power of discrimination (CPD) and combined power of exclusion for A-STR, a slight increase in CPD and combined mean exclusion chance for X-STR, and a marginal increase in discrimination capacity for Y-STR. Moreover, information data for 132 A-SNPs were acquired. As anticipated, our findings highlight the advantages of MPS in forensic genetic applications while contributing novel genetic data for Asian populations in forensic practice.</p>","PeriodicalId":45852,"journal":{"name":"Forensic Sciences Research","volume":"10 3","pages":"owae050"},"PeriodicalIF":1.8000,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12224613/pdf/","citationCount":"0","resultStr":"{\"title\":\"Parallel sequencing of 170 STR and 132 SNP markers using the FGID forensic four-in-one DNA typing kit on the DNBSEQ-G99RS platform.\",\"authors\":\"Xiaoyuan Zhen, Zhenmin Zhao, Ruocheng Xia, Xiling Liu, Hui Li, Yuzhen Gao, Baifang He, Chengtao Li, Ruiyang Tao\",\"doi\":\"10.1093/fsr/owae050\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Massive parallel sequencing (MPS) has rapidly emerged as a promising technique for forensic DNA typing due to its capacity to simultaneously detect numerous genetic markers and samples in a single reaction, allowing the direct acquisition of sequence information. In this current investigation, the FGID forensic four-in-one DNA typing kit was employed on the DNBSEQ-G99RS high-throughput sequencing platform to simultaneously analyse two types of forensic genetic markers-short tandem repeat (STR) and single nucleotide polymorphism (SNP). A total of 306 DNA markers, comprising Amelogenin, 66 autosomal STR (A-STR) loci, 29 X chromosomal STR (X-STR) loci, 75 Y chromosomal STR (Y-STR) loci, and 135 SNP (132 A-SNP and 3 Y-SNP) loci, were genotyped for 100 unrelated individual samples (50 males and 50 females). As a result, sequence-based STR typing identified 940 alleles on A-STRs, 378 alleles on X-STRs, and 519 alleles on Y-STRs. In comparison with length-based alleles, the number of unique alleles based on sequence increased by 58.18%. Additionally, 97 new sequence variations were observed at 29 STR loci, and MPS sequence information was obtained for the first time at 42 STR loci. Furthermore, when utilizing sequence-based data, forensic parameters exhibited a notable increase in combined power of discrimination (CPD) and combined power of exclusion for A-STR, a slight increase in CPD and combined mean exclusion chance for X-STR, and a marginal increase in discrimination capacity for Y-STR. Moreover, information data for 132 A-SNPs were acquired. As anticipated, our findings highlight the advantages of MPS in forensic genetic applications while contributing novel genetic data for Asian populations in forensic practice.</p>\",\"PeriodicalId\":45852,\"journal\":{\"name\":\"Forensic Sciences Research\",\"volume\":\"10 3\",\"pages\":\"owae050\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-08-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12224613/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Forensic Sciences Research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1093/fsr/owae050\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/9/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"MEDICINE, LEGAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Forensic Sciences Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1093/fsr/owae050","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/9/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"MEDICINE, LEGAL","Score":null,"Total":0}
Parallel sequencing of 170 STR and 132 SNP markers using the FGID forensic four-in-one DNA typing kit on the DNBSEQ-G99RS platform.
Massive parallel sequencing (MPS) has rapidly emerged as a promising technique for forensic DNA typing due to its capacity to simultaneously detect numerous genetic markers and samples in a single reaction, allowing the direct acquisition of sequence information. In this current investigation, the FGID forensic four-in-one DNA typing kit was employed on the DNBSEQ-G99RS high-throughput sequencing platform to simultaneously analyse two types of forensic genetic markers-short tandem repeat (STR) and single nucleotide polymorphism (SNP). A total of 306 DNA markers, comprising Amelogenin, 66 autosomal STR (A-STR) loci, 29 X chromosomal STR (X-STR) loci, 75 Y chromosomal STR (Y-STR) loci, and 135 SNP (132 A-SNP and 3 Y-SNP) loci, were genotyped for 100 unrelated individual samples (50 males and 50 females). As a result, sequence-based STR typing identified 940 alleles on A-STRs, 378 alleles on X-STRs, and 519 alleles on Y-STRs. In comparison with length-based alleles, the number of unique alleles based on sequence increased by 58.18%. Additionally, 97 new sequence variations were observed at 29 STR loci, and MPS sequence information was obtained for the first time at 42 STR loci. Furthermore, when utilizing sequence-based data, forensic parameters exhibited a notable increase in combined power of discrimination (CPD) and combined power of exclusion for A-STR, a slight increase in CPD and combined mean exclusion chance for X-STR, and a marginal increase in discrimination capacity for Y-STR. Moreover, information data for 132 A-SNPs were acquired. As anticipated, our findings highlight the advantages of MPS in forensic genetic applications while contributing novel genetic data for Asian populations in forensic practice.