Cui-Cui Wang, Zhen-Jing Li, Xiu-Hong Jia, Jian-Chang Li
{"title":"[萝卜硫素通过调控PI3K/Akt/mTOR信号通路对急性早幼粒细胞白血病细胞增殖和凋亡的影响]。","authors":"Cui-Cui Wang, Zhen-Jing Li, Xiu-Hong Jia, Jian-Chang Li","doi":"10.19746/j.cnki.issn.1009-2137.2025.03.002","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To investigate the impacts of sulforaphane (SPN) on cell proliferation and apoptosis in acute promyelogenous leukemia by regulating the PI3K/Akt/mTOR signaling pathway.</p><p><strong>Methods: </strong>NB4 cells were divided into 5 μmol/L SPN group, 10 μmol/L SPN group, 20 μmol/L SPN group, 740 Y-P (10 μmol/L) group and 20 μmol/L SPN+740 Y-P group, and the untreated NB4 cells were used as the control group. CCK-8, Hoechst 33342 staining, flow cytometry and monodansulfonylpentanediamine (MDC) were used to detect cell proliferation, apoptosis and autophagy, respectively. The expression levels of <i>Bcl-2, Bax, cyclin D1</i> and <i>LC3B</i> mRNA were detected by qRT-PCR. Western blot was used to detect the expression levels of PI3K/Akt/mTOR pathway-related proteins in NB4 cells.</p><p><strong>Results: </strong>Compared with the control group, the proliferation rate, <i>Bcl-2, cyclin D1</i> mRNA expressions, p-PI3K/PI3K, p-Akt/Akt, and p-mTOR/mTOR ratio were greatly increased in the 740 Y-P group (<i>P</i> < 0.05), the apoptosis rate, percentage of MDC positive, <i>Bax</i> and <i>LC3B</i> mRNA expression levels were greatly decreased (<i>P</i> < 0.05). The proliferation rate, <i>Bcl-2, cyclin D1</i> mRNA expression levels, p-PI3K/PI3K, p-Akt/Akt, and p-mTOR/mTOR ratio were greatly decreased in the 5 μmol/L SPN group, 10 μmol/L SPN group, and 20 μmol/L SPN group (<i>P</i> < 0.05), the apoptosis rate, percentage of MDC positive,<i>Bax</i> and <i>LC3B</i> mRNA expression levels were greatly increased, there were differences among different SPN treatment groups (<i>P</i> < 0.05). Compared with the 20 μmol/L SPN group, the proliferation rate, <i>Bcl-2, cyclin D1</i> mRNA expression levels, p-PI3K/PI3K, p-Akt/Akt, and p-mTOR/mTOR ratio were greatly increased in the 20 μmol/L SPN+740 Y-P group(<i>P</i> < 0.05), the apoptosis rate, percentage of MDC positive, <i>Bax</i> and <i>LC3B</i> mRNA expression levels were greatly decreased (<i>P</i> < 0.05). Compared with the 740 Y-P group, the proliferation rate, <i>Bcl-2, cyclin D1</i> mRNA expression levels, p-PI3K/PI3K, p-Akt/Akt, and p-mTOR/mTOR ratio in the 20 μmol/L SPN+740 Y-P group were greatly reduced (<i>P</i> < 0.05), the apoptosis rate, percentage of MDC positive, <i>Bax</i> and <i>LC3B</i> mRNA expression levels were greatly increased (<i>P</i> < 0.05).</p><p><strong>Conclusion: </strong>SPN reduces the proliferation of acute promyelocytic leukemia cells and promotes cells apoptosis by inhibiting the PI3K/Akt/mTOR signaling pathway.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 3","pages":"633-639"},"PeriodicalIF":0.0000,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Impacts of Sulforaphane on Cell Proliferation and Apoptosis in Acute Promyelogenous Leukemia by Regulating the PI3K/Akt/mTOR Signaling Pathway].\",\"authors\":\"Cui-Cui Wang, Zhen-Jing Li, Xiu-Hong Jia, Jian-Chang Li\",\"doi\":\"10.19746/j.cnki.issn.1009-2137.2025.03.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To investigate the impacts of sulforaphane (SPN) on cell proliferation and apoptosis in acute promyelogenous leukemia by regulating the PI3K/Akt/mTOR signaling pathway.</p><p><strong>Methods: </strong>NB4 cells were divided into 5 μmol/L SPN group, 10 μmol/L SPN group, 20 μmol/L SPN group, 740 Y-P (10 μmol/L) group and 20 μmol/L SPN+740 Y-P group, and the untreated NB4 cells were used as the control group. CCK-8, Hoechst 33342 staining, flow cytometry and monodansulfonylpentanediamine (MDC) were used to detect cell proliferation, apoptosis and autophagy, respectively. The expression levels of <i>Bcl-2, Bax, cyclin D1</i> and <i>LC3B</i> mRNA were detected by qRT-PCR. Western blot was used to detect the expression levels of PI3K/Akt/mTOR pathway-related proteins in NB4 cells.</p><p><strong>Results: </strong>Compared with the control group, the proliferation rate, <i>Bcl-2, cyclin D1</i> mRNA expressions, p-PI3K/PI3K, p-Akt/Akt, and p-mTOR/mTOR ratio were greatly increased in the 740 Y-P group (<i>P</i> < 0.05), the apoptosis rate, percentage of MDC positive, <i>Bax</i> and <i>LC3B</i> mRNA expression levels were greatly decreased (<i>P</i> < 0.05). The proliferation rate, <i>Bcl-2, cyclin D1</i> mRNA expression levels, p-PI3K/PI3K, p-Akt/Akt, and p-mTOR/mTOR ratio were greatly decreased in the 5 μmol/L SPN group, 10 μmol/L SPN group, and 20 μmol/L SPN group (<i>P</i> < 0.05), the apoptosis rate, percentage of MDC positive,<i>Bax</i> and <i>LC3B</i> mRNA expression levels were greatly increased, there were differences among different SPN treatment groups (<i>P</i> < 0.05). Compared with the 20 μmol/L SPN group, the proliferation rate, <i>Bcl-2, cyclin D1</i> mRNA expression levels, p-PI3K/PI3K, p-Akt/Akt, and p-mTOR/mTOR ratio were greatly increased in the 20 μmol/L SPN+740 Y-P group(<i>P</i> < 0.05), the apoptosis rate, percentage of MDC positive, <i>Bax</i> and <i>LC3B</i> mRNA expression levels were greatly decreased (<i>P</i> < 0.05). Compared with the 740 Y-P group, the proliferation rate, <i>Bcl-2, cyclin D1</i> mRNA expression levels, p-PI3K/PI3K, p-Akt/Akt, and p-mTOR/mTOR ratio in the 20 μmol/L SPN+740 Y-P group were greatly reduced (<i>P</i> < 0.05), the apoptosis rate, percentage of MDC positive, <i>Bax</i> and <i>LC3B</i> mRNA expression levels were greatly increased (<i>P</i> < 0.05).</p><p><strong>Conclusion: </strong>SPN reduces the proliferation of acute promyelocytic leukemia cells and promotes cells apoptosis by inhibiting the PI3K/Akt/mTOR signaling pathway.</p>\",\"PeriodicalId\":35777,\"journal\":{\"name\":\"中国实验血液学杂志\",\"volume\":\"33 3\",\"pages\":\"633-639\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中国实验血液学杂志\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.03.002\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中国实验血液学杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.03.002","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}
[Impacts of Sulforaphane on Cell Proliferation and Apoptosis in Acute Promyelogenous Leukemia by Regulating the PI3K/Akt/mTOR Signaling Pathway].
Objective: To investigate the impacts of sulforaphane (SPN) on cell proliferation and apoptosis in acute promyelogenous leukemia by regulating the PI3K/Akt/mTOR signaling pathway.
Methods: NB4 cells were divided into 5 μmol/L SPN group, 10 μmol/L SPN group, 20 μmol/L SPN group, 740 Y-P (10 μmol/L) group and 20 μmol/L SPN+740 Y-P group, and the untreated NB4 cells were used as the control group. CCK-8, Hoechst 33342 staining, flow cytometry and monodansulfonylpentanediamine (MDC) were used to detect cell proliferation, apoptosis and autophagy, respectively. The expression levels of Bcl-2, Bax, cyclin D1 and LC3B mRNA were detected by qRT-PCR. Western blot was used to detect the expression levels of PI3K/Akt/mTOR pathway-related proteins in NB4 cells.
Results: Compared with the control group, the proliferation rate, Bcl-2, cyclin D1 mRNA expressions, p-PI3K/PI3K, p-Akt/Akt, and p-mTOR/mTOR ratio were greatly increased in the 740 Y-P group (P < 0.05), the apoptosis rate, percentage of MDC positive, Bax and LC3B mRNA expression levels were greatly decreased (P < 0.05). The proliferation rate, Bcl-2, cyclin D1 mRNA expression levels, p-PI3K/PI3K, p-Akt/Akt, and p-mTOR/mTOR ratio were greatly decreased in the 5 μmol/L SPN group, 10 μmol/L SPN group, and 20 μmol/L SPN group (P < 0.05), the apoptosis rate, percentage of MDC positive,Bax and LC3B mRNA expression levels were greatly increased, there were differences among different SPN treatment groups (P < 0.05). Compared with the 20 μmol/L SPN group, the proliferation rate, Bcl-2, cyclin D1 mRNA expression levels, p-PI3K/PI3K, p-Akt/Akt, and p-mTOR/mTOR ratio were greatly increased in the 20 μmol/L SPN+740 Y-P group(P < 0.05), the apoptosis rate, percentage of MDC positive, Bax and LC3B mRNA expression levels were greatly decreased (P < 0.05). Compared with the 740 Y-P group, the proliferation rate, Bcl-2, cyclin D1 mRNA expression levels, p-PI3K/PI3K, p-Akt/Akt, and p-mTOR/mTOR ratio in the 20 μmol/L SPN+740 Y-P group were greatly reduced (P < 0.05), the apoptosis rate, percentage of MDC positive, Bax and LC3B mRNA expression levels were greatly increased (P < 0.05).
Conclusion: SPN reduces the proliferation of acute promyelocytic leukemia cells and promotes cells apoptosis by inhibiting the PI3K/Akt/mTOR signaling pathway.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
