Níura Madalena Bila, Carolina Orlando Vaso, Jenyffie Araújo Belizário, Mariana M Santoni Biasioli, Ana Marisa Fusco-Almeida, Luis R Martinez, Caroline Barcelos Costa-Orlandi, Maria José Soares Mendes-Giannini
{"title":"红毛癣菌在体外生物膜中生长时抑制白色念珠菌丝化及其基因表达。","authors":"Níura Madalena Bila, Carolina Orlando Vaso, Jenyffie Araújo Belizário, Mariana M Santoni Biasioli, Ana Marisa Fusco-Almeida, Luis R Martinez, Caroline Barcelos Costa-Orlandi, Maria José Soares Mendes-Giannini","doi":"10.1590/0074-02760240221","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Dermatomycoses are caused by various fungi, including dermatophytes and Candida species, which are the most prevalent in isolated or associated forms. A great number of virulence factors expressed by these fungi are important for infection, and biofilm formation leads to the persistence of these infections.</p><p><strong>Objectives: </strong>This work aimed to evaluate the dynamics of Candida albicans filamentation genes in biofilms formed by Candida albicans and Trichophyton rubrum.</p><p><strong>Methods: </strong>The effect of the supernatants on the biofilms was assessed by XTT reduction assay, confocal microscopy, and gene expression profile analysis by real-time polymerase chain reaction (RT-PCR).</p><p><strong>Findings: </strong>The supernatants did not reduce the metabolic activities or damage the topography of the monospecies biofilms but caused a reduction in their thickness. The filamentation of C. albicans was inhibited when both fungi were cultivated directly. The filamentation genes studied (CPH1, HWP1, and EFG1) were negatively modulated in C. albicans.</p><p><strong>Main conclusions: </strong>Our findings suggest that the antagonistic relationship shown by T. rubrum against C. albicans may be attributed to alterations of C. albicans filamentous genes.</p>","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"120 ","pages":"e240221"},"PeriodicalIF":2.5000,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12208673/pdf/","citationCount":"0","resultStr":"{\"title\":\"Trichophyton rubrum inhibits Candida albicans filamentation and its gene expression when grown in biofilms in vitro.\",\"authors\":\"Níura Madalena Bila, Carolina Orlando Vaso, Jenyffie Araújo Belizário, Mariana M Santoni Biasioli, Ana Marisa Fusco-Almeida, Luis R Martinez, Caroline Barcelos Costa-Orlandi, Maria José Soares Mendes-Giannini\",\"doi\":\"10.1590/0074-02760240221\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Dermatomycoses are caused by various fungi, including dermatophytes and Candida species, which are the most prevalent in isolated or associated forms. A great number of virulence factors expressed by these fungi are important for infection, and biofilm formation leads to the persistence of these infections.</p><p><strong>Objectives: </strong>This work aimed to evaluate the dynamics of Candida albicans filamentation genes in biofilms formed by Candida albicans and Trichophyton rubrum.</p><p><strong>Methods: </strong>The effect of the supernatants on the biofilms was assessed by XTT reduction assay, confocal microscopy, and gene expression profile analysis by real-time polymerase chain reaction (RT-PCR).</p><p><strong>Findings: </strong>The supernatants did not reduce the metabolic activities or damage the topography of the monospecies biofilms but caused a reduction in their thickness. The filamentation of C. albicans was inhibited when both fungi were cultivated directly. The filamentation genes studied (CPH1, HWP1, and EFG1) were negatively modulated in C. albicans.</p><p><strong>Main conclusions: </strong>Our findings suggest that the antagonistic relationship shown by T. rubrum against C. albicans may be attributed to alterations of C. albicans filamentous genes.</p>\",\"PeriodicalId\":18469,\"journal\":{\"name\":\"Memorias do Instituto Oswaldo Cruz\",\"volume\":\"120 \",\"pages\":\"e240221\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2025-06-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12208673/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Memorias do Instituto Oswaldo Cruz\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1590/0074-02760240221\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Memorias do Instituto Oswaldo Cruz","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1590/0074-02760240221","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Trichophyton rubrum inhibits Candida albicans filamentation and its gene expression when grown in biofilms in vitro.
Background: Dermatomycoses are caused by various fungi, including dermatophytes and Candida species, which are the most prevalent in isolated or associated forms. A great number of virulence factors expressed by these fungi are important for infection, and biofilm formation leads to the persistence of these infections.
Objectives: This work aimed to evaluate the dynamics of Candida albicans filamentation genes in biofilms formed by Candida albicans and Trichophyton rubrum.
Methods: The effect of the supernatants on the biofilms was assessed by XTT reduction assay, confocal microscopy, and gene expression profile analysis by real-time polymerase chain reaction (RT-PCR).
Findings: The supernatants did not reduce the metabolic activities or damage the topography of the monospecies biofilms but caused a reduction in their thickness. The filamentation of C. albicans was inhibited when both fungi were cultivated directly. The filamentation genes studied (CPH1, HWP1, and EFG1) were negatively modulated in C. albicans.
Main conclusions: Our findings suggest that the antagonistic relationship shown by T. rubrum against C. albicans may be attributed to alterations of C. albicans filamentous genes.
期刊介绍：
Memórias do Instituto Oswaldo Cruz is a journal specialized in microbes & their vectors causing human infections. This means that we accept manuscripts covering multidisciplinary approaches and findings in the basic aspects of infectious diseases, e.g. basic in research in prokariotes, eukaryotes, and/or virus. Articles must clearly show what is the main question to be answered, the hypothesis raised, and the contribution given by the study.
Priority is given to manuscripts reporting novel mechanisms and general findings concerning the biology of human infectious prokariotes, eukariotes or virus. Papers reporting innovative methods for diagnostics or that advance the basic research with these infectious agents are also welcome.
It is important to mention what we do not publish: veterinary infectious agents research, taxonomic analysis and re-description of species, epidemiological studies or surveys or case reports and data re-analysis. Manuscripts that fall in these cases or that are considered of low priority by the journal editorial board, will be returned to the author(s) for submission to another journal.
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