Yi Xiao, Jia Hui Wong, Nan Gao, Anna M Barron, Chelsea Violita Stanley, Aung Than, Lewen Zheng, Tong Ling, Dong-Sheng Li, Peng Chen
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Universal Artificial Urinary Biomarker Probe Enabled by an Aptamer-DNAzyme-Nanozyme Construct.
Artificial urinary biomarker probes (AUBPs) have recently emerged as a new class of diagnostic tools. However, they are applicable only to the biomarkers with catalytic activities or high reactivities. Here, we present a general modular design of AUBPs, integrating an aptamer for target recognition, DNAzyme for triggerable signal transduction, and nanozyme as a urinary artificial biomarker. For proof-of-concept demonstration, we developed an AUBP that is specifically and sensitively responsive to circulating an amyloid-β oligomer (AβO). After the nanoprobe was injected into circulation, colorimetric urinalysis was conducted to infer the circulating level of AβO for early diagnosis of Alzheimer's disease (AD). This approach can unambiguously distinguish AD mice from healthy ones, achieving a low detection limit of ∼2.8 pg/mL. It is ∼67 times more sensitive than the conventional enzyme-linked immunosorbent assay, owing to the cascaded signal amplification enabled by DNAzyme activity, enrichment by renal filtration, and chromogenic reaction catalyzed by nanozyme.
期刊介绍:
Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including:
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