月曲霉CL1中抗炎吡咯生物碱的发现

IF 2.6 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia Pub Date : 2025-07-03 DOI:10.1016/j.fitote.2025.106718

Qianqian Xu , Yuben Qiao , Chong Dai , Liangfang Pang , Liangxia Li , Yuanyuan Lu

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引用次数: 0

摘要

从弯孢菌CL1中分离到两种新型吡咯生物碱，pyrinoids A(1)和B(2)。它们的结构通过广泛的光谱方法，包括核磁共振、hresms、IR和UV，以及电子圆二色性（ECD）分析来确定绝对构型。两种化合物对脂多糖（LPS）诱导的RAW264.7细胞一氧化氮（NO）产生均有显著的抑制作用，IC50值分别为14.06±0.74 μM和12.64±3.82 μM。此外，pyrinoids A和B增加抗炎细胞因子IL-10的分泌，抑制促炎介质IL-6和PGE2。他们还调节了关键炎症标志物的转录水平，下调IL-6和iNOS，同时上调IL-10的表达。这些发现表明，pyrinoids A和B在巨噬细胞介导的途径中具有很好的抗炎潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Discovery of anti-inflammatory pyrrole alkaloids from Curvularia lunata CL1

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Discovery of anti-inflammatory pyrrole alkaloids from Curvularia lunata CL1

Two novel pyrrole alkaloids, pyrinoids A (1) and B (2), were isolated from the culture of Curvularia lunata CL1. Their structures were elucidated using extensive spectroscopic methods, including NMR, HRESIMS, IR, and UV, alongside electronic circular dichroism (ECD) analysis for absolute configuration determination. Both compounds exhibited significant inhibitory activity against lipopolysaccharide (LPS)-stimulated nitric oxide (NO) production in RAW264.7 cells, with IC₅₀ values of 14.06 ± 0.74 and 12.64 ± 3.82 μM, respectively. Additionally, pyrinoids A and B enhanced the secretion of the anti-inflammatory cytokine IL-10 and suppressed pro-inflammatory mediators IL-6 and PGE2. They also modulated the transcription levels of key inflammatory markers, downregulating IL-6 and iNOS while upregulating IL-10 expression. These findings suggest that pyrinoids A and B possess promising potential as anti-inflammatory agents for mitigating inflammation in macrophage-mediated pathways.

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来源期刊

Fitoterapia 医学-药学

CiteScore

5.80

自引率

2.90%

发文量

198

审稿时长

1.5 months

期刊介绍： Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas: 1. Characterization of active ingredients of medicinal plants 2. Development of standardization method for bioactive plant extracts and natural products 3. Identification of bioactivity in plant extracts 4. Identification of targets and mechanism of activity of plant extracts 5. Production and genomic characterization of medicinal plants biomass 6. Chemistry and biochemistry of bioactive natural products of plant origin 7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.