Tuytschaever Tessa , Mispelaere Marieke , Peeters Charlotte , De Canck Evelien , Vandamme Peter , Raes Katleen , Hulpiau Paco , Sampers Imca
{"title":"揭示单核细胞增生李斯特菌在冷冻马铃薯加工中的持久性:来自全基因组测序分析的见解","authors":"Tuytschaever Tessa , Mispelaere Marieke , Peeters Charlotte , De Canck Evelien , Vandamme Peter , Raes Katleen , Hulpiau Paco , Sampers Imca","doi":"10.1016/j.ijfoodmicro.2025.111334","DOIUrl":null,"url":null,"abstract":"<div><div><em>Listeria monocytogenes</em> persistence remains an issue in the food industry, including the frozen vegetable/potato processing industry. This study demonstrates the added value of whole genome sequencing (WGS) for persistence and transmission routes within food businesses. A total of 79 isolates from environmental samples (including employees' shoes), collected over three years (2020−2022), along with two intermediate product samples, and one end product sample<em>,</em> and submitted for WGS. The isolates were taken from in and around the freezing tunnel of a potato processing plant after blanching and frying. Of the 82 isolates, 56 were confirmed as <em>L. monocytogenes</em>, 17 as <em>L. innocua</em>, two as <em>Enterococcus faecalis</em>, and seven had unclear/mixed results using both TYGS (Type Strain Genome Server) and FastANI (Fast Average Nucleotide Identity). Clusters were identified using a 99.5 % ANI value cutoff. A single large <em>L. monocytogenes</em> cluster encompassed 55 of the 56 confirmed isolates and exhibited fewer than six allelic differences in pairwise comparisons based on cgMLST results. Two <em>L. innocua</em> clusters comprised 12 and five isolates, respectively. The results indicated <em>L. monocytogenes</em> persistence for at least three years and identified employees' shoes and a tool cart's wheels as potential transmission vehicles. The isolates of intermediate product samples belonged to the <em>L. monocytogenes</em> cluster, indicating post-contamination via the freezing tunnel. However, the end product isolate did not belong to the <em>L. monocytogenes</em> cluster, suggesting other high-risk areas within the facility. Various genes related to virulence, stress response, biofilm formation, and disinfectant resistance were identified in both <em>L. monocytogenes</em> and <em>L. innocua</em> clusters and in the <em>L. monocytogenes</em> end product isolate.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"441 ","pages":"Article 111334"},"PeriodicalIF":5.0000,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Unravelling Listeria monocytogenes persistence in frozen potato processing: Insights from whole genome sequencing analysis\",\"authors\":\"Tuytschaever Tessa , Mispelaere Marieke , Peeters Charlotte , De Canck Evelien , Vandamme Peter , Raes Katleen , Hulpiau Paco , Sampers Imca\",\"doi\":\"10.1016/j.ijfoodmicro.2025.111334\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div><em>Listeria monocytogenes</em> persistence remains an issue in the food industry, including the frozen vegetable/potato processing industry. This study demonstrates the added value of whole genome sequencing (WGS) for persistence and transmission routes within food businesses. A total of 79 isolates from environmental samples (including employees' shoes), collected over three years (2020−2022), along with two intermediate product samples, and one end product sample<em>,</em> and submitted for WGS. The isolates were taken from in and around the freezing tunnel of a potato processing plant after blanching and frying. Of the 82 isolates, 56 were confirmed as <em>L. monocytogenes</em>, 17 as <em>L. innocua</em>, two as <em>Enterococcus faecalis</em>, and seven had unclear/mixed results using both TYGS (Type Strain Genome Server) and FastANI (Fast Average Nucleotide Identity). Clusters were identified using a 99.5 % ANI value cutoff. A single large <em>L. monocytogenes</em> cluster encompassed 55 of the 56 confirmed isolates and exhibited fewer than six allelic differences in pairwise comparisons based on cgMLST results. Two <em>L. innocua</em> clusters comprised 12 and five isolates, respectively. The results indicated <em>L. monocytogenes</em> persistence for at least three years and identified employees' shoes and a tool cart's wheels as potential transmission vehicles. The isolates of intermediate product samples belonged to the <em>L. monocytogenes</em> cluster, indicating post-contamination via the freezing tunnel. However, the end product isolate did not belong to the <em>L. monocytogenes</em> cluster, suggesting other high-risk areas within the facility. Various genes related to virulence, stress response, biofilm formation, and disinfectant resistance were identified in both <em>L. monocytogenes</em> and <em>L. innocua</em> clusters and in the <em>L. monocytogenes</em> end product isolate.</div></div>\",\"PeriodicalId\":14095,\"journal\":{\"name\":\"International journal of food microbiology\",\"volume\":\"441 \",\"pages\":\"Article 111334\"},\"PeriodicalIF\":5.0000,\"publicationDate\":\"2025-06-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International journal of food microbiology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S016816052500279X\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of food microbiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S016816052500279X","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
Unravelling Listeria monocytogenes persistence in frozen potato processing: Insights from whole genome sequencing analysis
Listeria monocytogenes persistence remains an issue in the food industry, including the frozen vegetable/potato processing industry. This study demonstrates the added value of whole genome sequencing (WGS) for persistence and transmission routes within food businesses. A total of 79 isolates from environmental samples (including employees' shoes), collected over three years (2020−2022), along with two intermediate product samples, and one end product sample, and submitted for WGS. The isolates were taken from in and around the freezing tunnel of a potato processing plant after blanching and frying. Of the 82 isolates, 56 were confirmed as L. monocytogenes, 17 as L. innocua, two as Enterococcus faecalis, and seven had unclear/mixed results using both TYGS (Type Strain Genome Server) and FastANI (Fast Average Nucleotide Identity). Clusters were identified using a 99.5 % ANI value cutoff. A single large L. monocytogenes cluster encompassed 55 of the 56 confirmed isolates and exhibited fewer than six allelic differences in pairwise comparisons based on cgMLST results. Two L. innocua clusters comprised 12 and five isolates, respectively. The results indicated L. monocytogenes persistence for at least three years and identified employees' shoes and a tool cart's wheels as potential transmission vehicles. The isolates of intermediate product samples belonged to the L. monocytogenes cluster, indicating post-contamination via the freezing tunnel. However, the end product isolate did not belong to the L. monocytogenes cluster, suggesting other high-risk areas within the facility. Various genes related to virulence, stress response, biofilm formation, and disinfectant resistance were identified in both L. monocytogenes and L. innocua clusters and in the L. monocytogenes end product isolate.
期刊介绍:
The International Journal of Food Microbiology publishes papers dealing with all aspects of food microbiology. Articles must present information that is novel, has high impact and interest, and is of high scientific quality. They should provide scientific or technological advancement in the specific field of interest of the journal and enhance its strong international reputation. Preliminary or confirmatory results as well as contributions not strictly related to food microbiology will not be considered for publication.