Tom Normand-Gravier , Robert Solsona , Flavie Arnould , Roméo Deriaz , Christelle Bertrand-Gaday , Fabio Borrani , Henri Bernardi , Anthony M.J. Sanchez
{"title":"热干预和混合运动对蛋白质合成、核糖体生物发生和自噬的急性影响","authors":"Tom Normand-Gravier , Robert Solsona , Flavie Arnould , Roméo Deriaz , Christelle Bertrand-Gaday , Fabio Borrani , Henri Bernardi , Anthony M.J. Sanchez","doi":"10.1016/j.jtherbio.2025.104169","DOIUrl":null,"url":null,"abstract":"<div><div>The use of kaumatherapy (<em>i.e.</em> heat exposure like sauna-bathing) as passive intervention has become of growing interest to promote skeletal muscle adaptations such as strength gains and preservation of muscle mass. Importantly, the effects of a single exposure to heat (HE) in combination with hybrid exercises, designed to induce both aerobic and resistance adaptations (EX), on protein turnover remains unclear. The objective of this investigation was to evaluate the responses to HE, EX and their combination (EX + HE) on the expression of mRNA and protein related to proteosynthesis, ribosome biogenesis and content, as well as autophagy and cellular stress pathways. Eight-week-old male mice C57BL/6 J mice (n = 8 per condition) underwent acute HE (45min, 40 °C), EX (high-intensity inclined treadmill running) or EX + HE immediately after exercise. Mice were euthanized 240 min post-interventions and quadriceps muscles were harvested for analysis. Acute HE increased the mRNA expression of markers of ribosome biogenesis (POL1RA, UBF), but did not enhance ribosomal content (rRNA 18 S and 28 S) nor ribosomal transcription (pre-rRNA 45 S). Concerning the modulation of protein synthesis, EX induced an increase of MTORC1 downstream targets (P-S6K1, P-RPS6) and protein synthesis flux (assessed by puromycin incorporation) while HE alone did not and post-exercise HE had no additional effects. EX + HE also led to enhanced protein synthesis rates, but did not confer any additional benefit compared to EX alone. Finally, only EX + HE increased the autophagy flux marker LC3-B II/I, the microautophagy marker LAMP2A and phosphorylation level of P-NFκB Ser536. However, no changes in protein carbonylation were detected at this time point. These results suggest that acute post-exercise HE has no additional effects on protein synthesis at 4 h post-exercise but, when combined with EX, increases autophagy and P-NFκB Ser536, suggesting a heightened cellular stress response.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"131 ","pages":"Article 104169"},"PeriodicalIF":2.9000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Acute effects of heat intervention and hybrid exercise on protein synthesis, ribosome biogenesis and autophagy\",\"authors\":\"Tom Normand-Gravier , Robert Solsona , Flavie Arnould , Roméo Deriaz , Christelle Bertrand-Gaday , Fabio Borrani , Henri Bernardi , Anthony M.J. Sanchez\",\"doi\":\"10.1016/j.jtherbio.2025.104169\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The use of kaumatherapy (<em>i.e.</em> heat exposure like sauna-bathing) as passive intervention has become of growing interest to promote skeletal muscle adaptations such as strength gains and preservation of muscle mass. Importantly, the effects of a single exposure to heat (HE) in combination with hybrid exercises, designed to induce both aerobic and resistance adaptations (EX), on protein turnover remains unclear. The objective of this investigation was to evaluate the responses to HE, EX and their combination (EX + HE) on the expression of mRNA and protein related to proteosynthesis, ribosome biogenesis and content, as well as autophagy and cellular stress pathways. Eight-week-old male mice C57BL/6 J mice (n = 8 per condition) underwent acute HE (45min, 40 °C), EX (high-intensity inclined treadmill running) or EX + HE immediately after exercise. Mice were euthanized 240 min post-interventions and quadriceps muscles were harvested for analysis. Acute HE increased the mRNA expression of markers of ribosome biogenesis (POL1RA, UBF), but did not enhance ribosomal content (rRNA 18 S and 28 S) nor ribosomal transcription (pre-rRNA 45 S). Concerning the modulation of protein synthesis, EX induced an increase of MTORC1 downstream targets (P-S6K1, P-RPS6) and protein synthesis flux (assessed by puromycin incorporation) while HE alone did not and post-exercise HE had no additional effects. EX + HE also led to enhanced protein synthesis rates, but did not confer any additional benefit compared to EX alone. Finally, only EX + HE increased the autophagy flux marker LC3-B II/I, the microautophagy marker LAMP2A and phosphorylation level of P-NFκB Ser536. However, no changes in protein carbonylation were detected at this time point. These results suggest that acute post-exercise HE has no additional effects on protein synthesis at 4 h post-exercise but, when combined with EX, increases autophagy and P-NFκB Ser536, suggesting a heightened cellular stress response.</div></div>\",\"PeriodicalId\":17428,\"journal\":{\"name\":\"Journal of thermal biology\",\"volume\":\"131 \",\"pages\":\"Article 104169\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2025-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of thermal biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0306456525001263\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of thermal biology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0306456525001263","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOLOGY","Score":null,"Total":0}
Acute effects of heat intervention and hybrid exercise on protein synthesis, ribosome biogenesis and autophagy
The use of kaumatherapy (i.e. heat exposure like sauna-bathing) as passive intervention has become of growing interest to promote skeletal muscle adaptations such as strength gains and preservation of muscle mass. Importantly, the effects of a single exposure to heat (HE) in combination with hybrid exercises, designed to induce both aerobic and resistance adaptations (EX), on protein turnover remains unclear. The objective of this investigation was to evaluate the responses to HE, EX and their combination (EX + HE) on the expression of mRNA and protein related to proteosynthesis, ribosome biogenesis and content, as well as autophagy and cellular stress pathways. Eight-week-old male mice C57BL/6 J mice (n = 8 per condition) underwent acute HE (45min, 40 °C), EX (high-intensity inclined treadmill running) or EX + HE immediately after exercise. Mice were euthanized 240 min post-interventions and quadriceps muscles were harvested for analysis. Acute HE increased the mRNA expression of markers of ribosome biogenesis (POL1RA, UBF), but did not enhance ribosomal content (rRNA 18 S and 28 S) nor ribosomal transcription (pre-rRNA 45 S). Concerning the modulation of protein synthesis, EX induced an increase of MTORC1 downstream targets (P-S6K1, P-RPS6) and protein synthesis flux (assessed by puromycin incorporation) while HE alone did not and post-exercise HE had no additional effects. EX + HE also led to enhanced protein synthesis rates, but did not confer any additional benefit compared to EX alone. Finally, only EX + HE increased the autophagy flux marker LC3-B II/I, the microautophagy marker LAMP2A and phosphorylation level of P-NFκB Ser536. However, no changes in protein carbonylation were detected at this time point. These results suggest that acute post-exercise HE has no additional effects on protein synthesis at 4 h post-exercise but, when combined with EX, increases autophagy and P-NFκB Ser536, suggesting a heightened cellular stress response.
期刊介绍:
The Journal of Thermal Biology publishes articles that advance our knowledge on the ways and mechanisms through which temperature affects man and animals. This includes studies of their responses to these effects and on the ecological consequences. Directly relevant to this theme are:
• The mechanisms of thermal limitation, heat and cold injury, and the resistance of organisms to extremes of temperature
• The mechanisms involved in acclimation, acclimatization and evolutionary adaptation to temperature
• Mechanisms underlying the patterns of hibernation, torpor, dormancy, aestivation and diapause
• Effects of temperature on reproduction and development, growth, ageing and life-span
• Studies on modelling heat transfer between organisms and their environment
• The contributions of temperature to effects of climate change on animal species and man
• Studies of conservation biology and physiology related to temperature
• Behavioural and physiological regulation of body temperature including its pathophysiology and fever
• Medical applications of hypo- and hyperthermia
Article types:
• Original articles
• Review articles