对抗生物膜:噬菌体消除泌尿导管上由多重耐药的贺氏肠杆菌形成的生物膜。

IF 5.5 3区 医学 Q1 IMMUNOLOGY
Martyna Cieślik, Michał Wójcicki, Paweł Migdał, Ilona Grygiel, Olaf Bajrak, Filip Orwat, Andrzej Górski, Ewa Jończyk-Matysiak
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引用次数: 0

摘要

阴沟肠杆菌复合体(ECC)是一种常见的医院病原体,与各种人类感染有关,目前包括几个物种,包括阴沟肠杆菌和霍马氏肠杆菌。能够在各种生物和非生物表面产生生物膜的菌株构成了特殊的威胁。因此,我们将研究重点放在了三株霍马氏肠杆菌中,这些菌株的基因组中存在生物膜相关基因:fimA、csgA、csgD和sdiA。这些菌株在泌尿导管上形成生物膜的动力学取决于导管材料(硅或乳胶)、温度(24°C或37°C)和孵育时间。对噬菌体破坏生物膜形成的能力进行了评估,发现在这一过程的早期阶段应用噬菌体时最有效。此外,噬菌体和/或银或铜纳米颗粒对现有生物膜的破坏是菌株依赖的。在某些情况下,与肠杆菌特异性噬菌体孵育可以几乎完全根除附着在泌尿导管上的三天生物膜。在两种肠杆菌特异性噬菌体的基因组中,鉴定出编码具有裂解活性蛋白的区域(分别在Entb_43噬菌体基因组中有6个区域,在Entb_45噬菌体基因组中有4个区域)。这些结果强调了生物膜相关感染的威胁,但也表明噬菌体具有多方面的抗生物膜活性,应在临床实践中加以考虑。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Fighting biofilm: bacteriophages eliminate biofilm formed by multidrug-resistant Enterobacter hormaechei on urological catheters.

The Enterobacter cloacae complex (ECC) is a prevalent nosocomial pathogen associated with various human infections, which currently comprises several species, including Enterobacter cloacae and Enterobacter hormaechei. Strains capable of producing biofilm on various biotic and abiotic surfaces pose a particular threat. Therefore, we focused on three E. hormaechei strains in whose genomes the presence of the biofilm-related genes: fimA, csgA, csgD, and sdiA was confirmed. Kinetic of biofilm formation by these strains on urological catheters depended on the catheter material (silicon or latex), temperature (24 °C or 37 °C) and incubation time. The ability of phages to disrupt biofilm formation was assessed and found to be the most effective when phages were applied at an early stages of this process. Moreover, destruction of existing biofilm by bacteriophages and/or silver or copper nanoparticles was strain-dependent. Incubation with Enterobacter-specific bacteriophages enabled, in some cases, almost complete eradication of three-day biofilms attached to urological catheters. In genomes of two Enterobacter-specific bacteriophages the presence of regions encoding proteins with lytic activity were identified (6 regions in Entb_43 phage and 4 regions in Entb_45 phage genomes, respectively). These results highlight the threat of biofilm-related infections, but also indicate the multifaceted anti-biofilm activity of bacteriophages, which should be considered for useful in clinical practice.

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来源期刊
CiteScore
10.60
自引率
0.00%
发文量
29
审稿时长
1 months
期刊介绍: Medical Microbiology and Immunology (MMIM) publishes key findings on all aspects of the interrelationship between infectious agents and the immune system of their hosts. The journal´s main focus is original research work on intrinsic, innate or adaptive immune responses to viral, bacterial, fungal and parasitic (protozoan and helminthic) infections and on the virulence of the respective infectious pathogens. MMIM covers basic, translational as well as clinical research in infectious diseases and infectious disease immunology. Basic research using cell cultures, organoid, and animal models are welcome, provided that the models have a clinical correlate and address a relevant medical question. The journal also considers manuscripts on the epidemiology of infectious diseases, including the emergence and epidemic spreading of pathogens and the development of resistance to anti-infective therapies, and on novel vaccines and other innovative measurements of prevention. The following categories of manuscripts will not be considered for publication in MMIM: submissions of preliminary work, of merely descriptive data sets without investigation of mechanisms or of limited global interest, manuscripts on existing or novel anti-infective compounds, which focus on pharmaceutical or pharmacological aspects of the drugs, manuscripts on existing or modified vaccines, unless they report on experimental or clinical efficacy studies or provide new immunological information on their mode of action, manuscripts on the diagnostics of infectious diseases, unless they offer a novel concept to solve a pending diagnostic problem, case reports or case series, unless they are embedded in a study that focuses on the anti-infectious immune response and/or on the virulence of a pathogen.
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