通过固体脂质纳米颗粒持续递送多替格拉韦钠以更好地管理艾滋病毒/艾滋病。

IF 1 4区 医学 Q4 IMMUNOLOGY
Mohit Singh, Pawan Kedar, Abhishek Kanugo, Amit Bukkawar
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引用次数: 0

摘要

导言:人类免疫缺陷病毒(艾滋病毒)是导致获得性免疫缺陷综合症(艾滋病)的主要健康问题,全球有超过3990万人感染艾滋病毒。多替格拉韦钠是一种亲脂性化合物,对数P值为2.2。目前的研究旨在通过固体脂质纳米颗粒增加多替格拉韦钠的溶解度、溶解度和治疗效果。方法:采用高速均质和探针超声法制备多替格拉韦钠固体脂质纳米粒。研究了固体脂质和表面活性剂对SLN形成的影响。采用Box-Behnken设计模型对SLN进行优化。考察了脂质、表面活性剂和均质速度对颗粒大小和包封效率的影响。将胶体分散体冻干,并评估其加速稳定性。结果:傅里叶变换红外光谱(FTIR)证实了药物辅料之间的相互作用。用差示扫描量热法(DSC)检查了其热行为和结晶性质。在几种固体脂质中,单硬脂酸甘油酯(GMS)的溶解度最高。吐温20稳定了胶体分散。讨论:因此,采用Box-Behnken设计模型和方差分析(ANOVA)模型。粒径和截留效率的p值分别为0.0050和0.0010。优化后的批D5粒径为189 nm, ZP为-24.6 mV,包封效率为85.94%,释药率为87.02%。对优化后的D5批进行了进一步的冻干和扫描电镜分析,确定了多替格拉韦钠的单核苷酸多态性在纳米级范围内。结论:固体脂质纳米颗粒具有明显的溶解性和溶出性。24小时的持续给药减少了给药频率,最大限度地减少了病毒载量,从而有效地治疗HIV,从而提高了患者的舒适度和依从性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Sustained Delivery of Dolutegravir Sodium for Better Management of HIV/AIDS via Solid Lipid Nanoparticles.

Introduction: Human immunodeficiency virus (HIV) is a primary health concern that leads to Acquired immunodeficiency syndrome (AIDS), with more than 39.9 million people living with HIV globally. Dolutegravir sodium is a lipophilic compound with a log P value of 2.2. The current research aimed at augmenting the solubility, dissolution, and therapeutic benefits of Dolutegravir sodium through Solid lipid nanoparticles.

Methods: The solid lipid nanoparticles (SLN) of Dolutegravir sodium were developed using high-speed homogenization and probe sonication methods. The solid lipid and surfactant were scrutinized for the development of SLN. The optimization of SLN was established using the Box-Behnken design model. The effects of lipid, surfactant, and homogenization speed on particle size and entrapment efficiency were evaluated. The colloidal dispersion was lyophilized, and accelerated stability was assessed.

Results: Fourier Transform Infrared Spectroscopy (FTIR) confirmed the interactions between the drug excipients. The thermal behavior and crystalline nature were checked with Differential Scanning Calorimetry (DSC). Among the several tested solid lipids, the highest solubility was observed in glyceryl monostearate (GMS). The colloidal dispersion was stabilized by the Tween 20.

Discussion: Accordingly, the Box-Behnken design model and the analysis of variance (ANOVA) model were applied. The p-values for the particle size and entrapment efficiency were 0.0050 and 0.0010, respectively. The optimized batch D5 showed a particle size of 189 nm, zeta potential (ZP) of -24.6 mV, entrapment efficiency of 85.94 %, and drug release of 87.02%. The optimized batch D5 was further lyophilized and analyzed with scanning electron microscopy (SEM), which confirmed the nanoscale range for SLN of Dolutegravir sodium.

Conclusion: A significant enhancement in solubility and dissolution was achieved with the solid lipid nanoparticles. The sustained delivery of 24 hours reduces the dosage frequency and minimizes the viral load for the effective therapy of HIV, thereby improving patients' comfort and compliance.

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来源期刊
Current HIV Research
Current HIV Research 医学-病毒学
CiteScore
1.90
自引率
10.00%
发文量
81
审稿时长
6-12 weeks
期刊介绍: Current HIV Research covers all the latest and outstanding developments of HIV research by publishing original research, review articles and guest edited thematic issues. The novel pioneering work in the basic and clinical fields on all areas of HIV research covers: virus replication and gene expression, HIV assembly, virus-cell interaction, viral pathogenesis, epidemiology and transmission, anti-retroviral therapy and adherence, drug discovery, the latest developments in HIV/AIDS vaccines and animal models, mechanisms and interactions with AIDS related diseases, social and public health issues related to HIV disease, and prevention of viral infection. Periodically, the journal invites guest editors to devote an issue on a particular area of HIV research of great interest that increases our understanding of the virus and its complex interaction with the host.
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