柠檬-薄荷精油纳米乳混合物的研制:对芽孢杆菌抑制、A549肺癌细胞活力、细胞周期进展和遗传毒性的影响。

IF 2.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Heba Mohamed Fahmy, Amany Mohamed Hamad
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引用次数: 0

摘要

对治疗的日益增加的耐药性和单一药物对癌症和食源性细菌的有限有效性突出了使用多种天然成分进行治疗协同的必要性。本研究旨在研制一种混合柠檬(LEO)和薄荷(PEO)两种植物精油的纳米乳液(NE),用于抗癌和抗菌测试。通过GC-MS分析PEO和LEO的化学成分,确定其主要成分为d -柠檬烯(77.89%)和薄荷醇(56.63%)。经FTIR证实,纳米粒子的粒径分别为57.2 nm (LEO)、104.2 nm (PEO)和44.3 nm (blend)。对A549肺癌细胞的细胞活力、DNA损伤和细胞周期进展进行了评估,结果显示LEO (1.7 mg/mL)、PEO (4.9 mg/mL)和混合物(2.5 mg/mL)治疗后,细胞活力降低了50%。混合NE可诱导细胞凋亡,阻断细胞周期s期。对蜡样芽孢杆菌、枯草芽孢杆菌和铜绿假单胞菌的抑菌活性进行了评价。MIC方面,混合NE (20 mg/mL)对枯草芽孢杆菌的抑制效果优于LEO-NE (40 mg/mL)和PEO-NE (80 mg/mL)。透射电镜分析证实了细菌裂解,支持了混合NE的优越抗菌效果。这种协同作用有望增强癌症治疗和抗菌应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Development of a Lemon-peppermint Essential Oil Nanoemulsion Blend: Effects on Bacillus spp. Inhibition, A549 Lung Cancer Cell Viability, Cell Cycle Progression, and Genotoxicity.

The increasing resistance to treatments and limited effectiveness of singular medications against cancer and foodborne bacteria highlight the need for therapeutic synergy using multiple natural components. This study aimed to develop a blend nanoemulsion (NE) combining two plant essential oils, lemon (LEO) and peppermint (PEO), for anticancer and antibacterial testing. The chemical composition of PEO and LEO was analyzed by GC-MS, identifying D-limonene (77.89%) and menthol (56.63%) as their primary constituents. The NEs were prepared with droplet sizes of 57.2 (LEO), 104.2 (PEO), and 44.3 nm (blend), confirmed by FTIR. Cellular viability, DNA damage, and cell cycle progression in A549 lung cancer cells were assessed, showing a 50% reduction in viability after treatment with LEO (1.7 mg/mL), PEO (4.9 mg/mL), and the blend (2.5 mg/mL). Blend NE induced apoptosis and halted the S-phase of the cell cycle. Antibacterial activity was evaluated against Bacillus cereus, Bacillus subtilis, and Pseudomonas aeruginosa. Regarding MIC, blend NE (20 mg/mL) was more effective than LEO-NE (40 mg/mL) and PEO-NE (80 mg/mL) against B. subtilis. TEM analysis confirmed bacterial lysis, supporting the blend NE's superior antimicrobial effects. This synergy holds promise for enhanced cancer treatment and antibacterial applications.

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来源期刊
Cell Biochemistry and Biophysics
Cell Biochemistry and Biophysics 生物-生化与分子生物学
CiteScore
4.40
自引率
0.00%
发文量
72
审稿时长
7.5 months
期刊介绍: Cell Biochemistry and Biophysics (CBB) aims to publish papers on the nature of the biochemical and biophysical mechanisms underlying the structure, control and function of cellular systems The reports should be within the framework of modern biochemistry and chemistry, biophysics and cell physiology, physics and engineering, molecular and structural biology. The relationship between molecular structure and function under investigation is emphasized. Examples of subject areas that CBB publishes are: · biochemical and biophysical aspects of cell structure and function; · interactions of cells and their molecular/macromolecular constituents; · innovative developments in genetic and biomolecular engineering; · computer-based analysis of tissues, cells, cell networks, organelles, and molecular/macromolecular assemblies; · photometric, spectroscopic, microscopic, mechanical, and electrical methodologies/techniques in analytical cytology, cytometry and innovative instrument design For articles that focus on computational aspects, authors should be clear about which docking and molecular dynamics algorithms or software packages are being used as well as details on the system parameterization, simulations conditions etc. In addition, docking calculations (virtual screening, QSAR, etc.) should be validated either by experimental studies or one or more reliable theoretical cross-validation methods.
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