氟化二胺银对牙本质和低矿化牙釉质渗透性的影响不同。

IF 6.3 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials Pub Date : 2025-07-01 DOI:10.1016/j.dental.2025.06.011

Conrad Chou , Misun Kang , Dilworth Y. Parkinson , Rosalyn Sulyanto , Sunita P. Ho

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引用次数: 0

摘要

目的：探讨氟化二胺银（SDF）的渗透性与低矿化牙釉质和龋齿牙本质的矿物密度和元素组成的关系。方法：采用微x线计算机断层扫描技术，对体内和未进行SDF处理的人乳牙的牙釉质和牙本质，以及体外和未进行SDF处理的恒磨牙的低矿化牙釉质进行扫描。利用x射线荧光探针生成生物金属（钙、锌）、磷和银的空间图。渗透率采用多孔微观结构分析软件计算。结果：经sdf处理的龋齿牙本质渗透性比未处理的正常牙本质低14.3 % (6.39e-15 ± 3.01e-15 m²vs 7.46e-15 ± 1.82e-15 m²；P 意义：本研究引入了一种测量牙组织通透性的计算方法，并证明了SDF可以显著降低龋牙本质的通透性，但对完整的低矿化牙釉质没有作用。该研究揭示了生物金属锌定位可以改变牙本质和牙釉质的渗透性，为龋病和低矿化的病理生物学机制提供了新的见解。

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Silver diamine fluoride differentially affects dentin and hypomineralized enamel permeabilities

Objectives

To investigate the physicochemical effect of silver diamine fluoride (SDF) by correlating permeability with mineral density and elemental composition of hypomineralized enamel and carious dentin.

Methods

Enamel and dentin from human carious primary teeth with and without SDF treatment in-vivo, and hypomineralized enamel from permanent molars with and without SDF treatment in-vitro were scanned using micro X-ray computed tomography. Spatial maps of biometals (calcium, zinc), phosphorus, and silver were generated using X-ray fluorescence microprobe. Permeabilities were computed using Porous Microstructure Analysis software.

Results

The intrinsic permeability of SDF-treated carious dentin was 14.3 % lower than untreated sound dentin (6.39e-15 ± 3.01e-15 m² vs 7.46e-15 ± 1.82e-15 m²; P < 0.0001), while untreated carious dentin was 98.4 % higher (1.48e-14 ± 7.11e-15 m²; P < 0.0001). SDF-treated and untreated transparent dentin showed similar reduced permeabilities (75.6 % and 78.4 % lower than untreated sound dentin, respectively; P = 0.93). Severely hypomineralized enamel showed permeability reaching 108.1 % of adjacent sound dentin (5.71e-15 ± 2.04e-15 m² vs 5.28e-15 ± 1.30e-15 m²; P = 0.1409) and was significantly higher than mildly hypomineralized enamel (1.39e-15 ± 1.04e-15 m²; P < 0.0001). SDF treatment did not significantly impact the permeability of severely hypomineralized enamel (12.4 % reduction; P = 0.07). Principal component regression identified Zn level as a significant effector of tissue permeabilities in carious primary teeth (P < 0.0001).

Significance

This study introduces a computational method to measure dental tissue permeability, and demonstrates that SDF significantly reduces permeability in carious dentin but not intact hypomineralized enamel. The study reveals biometal Zn localization can alter dentin and enamel permeabilities, providing new insights into pathobiological mechanisms underlying caries and hypomineralization.

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来源期刊

Dental Materials 工程技术-材料科学：生物材料

CiteScore

9.80

自引率

10.00%

发文量

290

审稿时长

67 days

期刊介绍： Dental Materials publishes original research, review articles, and short communications. Academy of Dental Materials members click here to register for free access to Dental Materials online. The principal aim of Dental Materials is to promote rapid communication of scientific information between academia, industry, and the dental practitioner. Original Manuscripts on clinical and laboratory research of basic and applied character which focus on the properties or performance of dental materials or the reaction of host tissues to materials are given priority publication. Other acceptable topics include application technology in clinical dentistry and dental laboratory technology. Comprehensive reviews and editorial commentaries on pertinent subjects will be considered.