Shifa Shaffique, Muhammad Farooq, Arjun Adhikari, Muhammad Aaqil Khan, Sang-Mo Kang, In-Jung Lee
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引用次数: 0
摘要
有益微生物是提高作物抗逆性的有力生物工具。本研究旨在分离植物生长促进耐胁迫细菌,利用高通量新一代测序和表达谱分析对其进行鉴定,并评估其在大豆(Glycine max (L.))改良中的潜力。铅胁迫下Merr.)的生长发育。我们鉴定蜡样芽孢杆菌SH-10是一种耐金属的根细菌,能够耐受铅浓度超过75 mg/kg。SH-10接种于铅胁迫下的大豆植株后,显著提高了大豆对磷和钙的吸收,减少了铅的积累。这种减少与应激信号蛋白GmCYP82A3的表达增加有关。SH-10还通过降低芽部脱落酸(ABA)水平和促进水杨酸(SA)的生物合成来调节植物激素平衡。这些激素变化与ABA生物合成相关基因(GmNCED1、NCED3、GmbZIP)的下调和SA生物合成基因GmPAL1的上调相对应。此外,SH-10显著减轻了铅暴露植物的氧化胁迫,同时增强了抗氧化活性。本研究还确定了两种植物激素的合成途径:(1)Terpenes→β-胡萝卜素→黄嘌呤素→ABA; (2) Terpenes→香叶基二磷酸→赤霉素酸。这些发现表明蜡样芽孢杆菌SH-10是一种很有前途的生物肥料,可以减轻作物中的铅毒性。
Genomic and Functional Insights Into Bacillus cereus SH-10 for Mitigating Lead Stress in Soybean.
Beneficial microbes serve as powerful biological tools for enhancing stress tolerance in crops. This study aimed to isolate stress-tolerant, plant growth-promoting bacterium, characterise it using high-throughput next-generation sequencing and expression profiling and assess its potential in improving soybean (Glycine max (L.) Merr.) growth and development under lead (Pb) stress. We identified Bacillus cereus SH-10 as a metal-resistant rhizobacterium capable of tolerating Pb concentrations exceeding 75 mg/kg. When inoculated into soybean plants under Pb stress, SH-10 significantly improved phosphorus and calcium uptake and reduced Pb accumulation. This reduction was associated with increased expression of the stress signalling protein GmCYP82A3. SH-10 also modulated the phytohormone balance by lowering abscisic acid (ABA) levels in shoots and enhancing salicylic acid (SA) biosynthesis. These hormonal shifts corresponded with the downregulation of ABA biosynthesis-related genes (GmNCED1, NCED3, GmbZIP) and the upregulation of the SA biosynthesis gene GmPAL1. Furthermore, SH-10 significantly mitigated oxidative stress in Pb-exposed plants, while enhancing antioxidant activities. The study also identified two phytohormone biosynthesis pathways in rhizobacteria: (1) Terpenes → β-carotene → Xanthoxin → ABA, and (2) Terpenes → Geranylgeranyl diphosphate → gibberellic acid. These findings highlight Bacillus cereus SH-10 as a promising biofertiliser that alleviates Pb toxicity in crops.
期刊介绍:
Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.