基于氨基偶氮苯改性明胶与聚环糊精主客体相互作用设计具有抗菌性能的自愈水凝胶以延长酪氨酸酶的保质期。

IF 5.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomacromolecules Pub Date : 2025-07-14 DOI:10.1021/acs.biomac.5c00346

Shaghayegh Vakili , Hamed Azadfar , Ebrahim Ahmadi , Zahra Mohamadnia , Atiyeh Mahdavi , Faezeh Hanifeh

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引用次数: 0

摘要

本文设计并合成了一种自修复水凝胶来固定酪氨酸酶（Tyr），以解决提高酶性能的需要。水凝胶的自愈特性保证了结构的完整性，同时其酶固定能力显著提高了酶的稳定性和活性。该水凝胶通过聚环糊精（PCD）和氨基偶氮苯改性明胶（Gel-AZO）的主客体相互作用合成，并在甲基丙烯酸缩水甘油酯改性明胶（gm -明胶）和丙烯酰氧乙基三甲基氯化铵（DAC）存在下化学交联形成双网络结构。固定化酶保持了游离酶94.56%的活性，在不同pH和温度下保持稳定，在70℃时保持70%的活性。可重复使用性测试表明，酶活性在六个循环中保持不变。该水凝胶对金黄色葡萄球菌和大肠杆菌具有抗菌活性，细胞活力维持在80%以上，溶血活性低于5%。这些结果突出了这种自愈水凝胶在需要稳定酶性能的生物医学和工业环境中的潜在适用性。

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Designing Self-Healing Hydrogels with Antibacterial Property Based on Host–Guest Interactions between Aminoazobenzene-Modified Gelatin and Polycyclodextrin for Prolonging the Shelf Life of Tyrosinase Enzyme

Here, a self-healing hydrogel was designed and synthesized to immobilize tyrosinase enzyme (Tyr), addressing the need for improved enzyme performance. The self-healing properties of the hydrogel ensured structural integrity, while its enzyme immobilization capability significantly enhanced enzyme stability and activity. The hydrogel was synthesized via host–guest interactions between polycyclodextrin (PCD) and aminoazobenzene-modified gelatin (Gel-AZO), and chemically cross-linked in the presence of glycidyl methacrylate-modified gelatin (GM-Gelatin) and acryloyloxyethyltrimethylammonium chloride (DAC) to form a dual-network structure. The immobilized enzyme retained 94.56% of the free enzyme’s activity and remained stable under varying pH and temperatures, maintaining 70% activity at 70 °C. Reusability tests showed preserved enzymatic activity over six cycles. The hydrogel exhibited antibacterial activity against Staphylococcus aureus and Escherichia coli, maintained cell viability above 80%, and showed hemolytic activity below 5%. These results highlight the potential applicability of this self-healing hydrogel in biomedical and industrial settings that require stable enzymatic performance.

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来源期刊

Biomacromolecules 化学-高分子科学

CiteScore

10.60

自引率

4.80%

发文量

417

审稿时长

1.6 months

期刊介绍： Biomacromolecules is a leading forum for the dissemination of cutting-edge research at the interface of polymer science and biology. Submissions to Biomacromolecules should contain strong elements of innovation in terms of macromolecular design, synthesis and characterization, or in the application of polymer materials to biology and medicine. Topics covered by Biomacromolecules include, but are not exclusively limited to: sustainable polymers, polymers based on natural and renewable resources, degradable polymers, polymer conjugates, polymeric drugs, polymers in biocatalysis, biomacromolecular assembly, biomimetic polymers, polymer-biomineral hybrids, biomimetic-polymer processing, polymer recycling, bioactive polymer surfaces, original polymer design for biomedical applications such as immunotherapy, drug delivery, gene delivery, antimicrobial applications, diagnostic imaging and biosensing, polymers in tissue engineering and regenerative medicine, polymeric scaffolds and hydrogels for cell culture and delivery.