一种具有显著活性氧清除活性的新型苹果酸-半胱氨酸配体的Mn和Cu配合物

IF 4.6 3区 化学 Q2 CHEMISTRY, MULTIDISCIPLINARY
RSC Advances Pub Date : 2025-07-04 DOI:10.1039/D5RA02118A
Saeedeh Khadivi-Derakhshan, Mahtab Pirouzmand, Jafar Soleymani and Mehdi D. Esrafili
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引用次数: 0

摘要

科学界对研究抗氧化活性非常感兴趣,使用各种检测方法来治疗氧化应激和减少自由基的有害影响。半胱氨酸,一种含硫化合物,可以治疗氧化应激。本研究的主要目的是通过l -半胱氨酸与苹果酸及其金属配合物铜和锰的结合,制备一种新型配体。下面的讨论重点是复合物如何模拟天然抗氧化酶,并解释它们如何用于去除活性氧。采用FT-IR、UV-vis、TGA、CHNS、EDX、SEM、质量和荧光等多种方法对合成进行验证。这些技术表明在与β-CD络合和整合后结构发生了变化。用分光光度法测定了化合物在不同条件下的过氧化物酶、过氧化氢酶和超氧化物歧化酶样活性。采用HFF-2细胞和MTT试验评价细胞毒性。碳、氢、氮、硫的含量分别为39.48、4.7、6.58、15.23,配体式为C7H11NSO6。金属相互作用的红外光谱信号的变化,特别是在羧酸区,表明显著的金属-配体键合。结果表明,β-CD修饰的配合物,特别是cu -配合物/β-CD和mn -配合物/β-CD具有较好的抗氧化活性(分别为91%和78%)。它们在清除O2˙−自由基和将H2O2分解为安全副产物方面表现较好。此外,mn -复合物/β-CD具有较小的细胞毒性。综上所述,所制备的复合物具有良好的抗氧化剂和治疗氧化应激相关疾病的潜力,尤其是β-CD衍生物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Mn and Cu complexes of a novel malic acid–cysteine ligand with remarkable ROS scavenging activity†

Mn and Cu complexes of a novel malic acid–cysteine ligand with remarkable ROS scavenging activity†

The scientific community is very interested in investigating antioxidant activity using various assays to treat oxidative stress and reduce the harmful effects of free radicals. Cysteine, a sulfur-containing compound can treat oxidative stress. The primary objective of this study was to create a novel ligand through the combination of L-cysteine with malic acid and its metal complexes with copper and manganese. The following discussion focuses on how the complexes mimic natural antioxidant enzymes and explains how they could be used to remove reactive oxygen species. Various methods were used to verify the synthesis, including FT-IR, UV-vis, TGA, CHNS, EDX, SEM, mass, and fluorescence. These techniques indicated structural changes upon complexation and integration with β-CD. Spectrophotometric tests were used to evaluate the compounds' peroxidase-, catalase-, and superoxide dismutase-like activities under different conditions. Cytotoxicity was evaluated using HFF-2 cells and the MTT test. The amounts of carbon, hydrogen, nitrogen, and sulfur were 39.48, 4.7, 6.58, and 15.23, and the ligand formula is C7H11NSO6. Changes in infrared spectral signals upon metal interaction, particularly in the carboxylate region, indicate significant metal–ligand bonding. The results showed that the β-CD-modified complexes, especially Cu-complex/β-CD and Mn-complex/β-CD, had better antioxidant activity (91% and 78%, respectively). They were better at scavenging O2˙ radicals and breaking down H2O2 into safe byproducts. Additionally, Mn-complex/β-CD was less cytotoxic. In conclusion, the produced complexes are promising antioxidants and potential treatments for diseases linked to oxidative stress, especially the β-CD derivatives.

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来源期刊
RSC Advances
RSC Advances chemical sciences-
CiteScore
7.50
自引率
2.60%
发文量
3116
审稿时长
1.6 months
期刊介绍: An international, peer-reviewed journal covering all of the chemical sciences, including multidisciplinary and emerging areas. RSC Advances is a gold open access journal allowing researchers free access to research articles, and offering an affordable open access publishing option for authors around the world.
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