浓缩尿液作为宫颈涂片样本的替代方法，便于在大量人群中筛查HPV

IF 4 3区医学 Q1 INFECTIOUS DISEASES

Journal of Infection and Public Health Pub Date : 2025-06-02 DOI:10.1016/j.jiph.2025.102857

Neval Yurttutan Uyar , Harika Bodur , Merve Olcen Erdem , Irem Ayse Kanneci Altinisik , Ece Aksoy , Tuba Polat , Figen Demir , Osman Acar , Boran Aksakal , Gulnur Alizade , Narmin Nadirova , Alara Apa , Ozge Can , Tanil Kocagoz

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引用次数: 0

摘要

人乳头瘤病毒（HPV）感染是宫颈癌的主要原因，需要有效的筛查方法，特别是在人口众多和资源有限的情况下。目前基于子宫颈涂片的筛查面临着与可及性、侵入性和患者依从性相关的挑战。本研究探讨了使用浓缩尿液样本作为HPV检测的非侵入性替代方法的可行性。方法收集126例患者的首次空尿和宫颈拭子。在RT-PCR检测HPV之前，使用生物液体浓缩器MyMagiCon®浓缩尿液样本。结果浓缩尿样HPV检测结果与宫颈涂片检测结果基本一致（Fleiss kappa = 0.796, p <； 0.0001）。浓缩尿液样本显示HPV检测比未浓缩尿液增加17. %。结论这种无创的新方法在可及性和患者接受度方面具有显著优势，有可能提高筛查覆盖率和早期检出率，特别是在服务不足的人群中。进一步的研究需要在更大、更多样化的人群中验证这些发现，并优化方法以提高敏感性和特异性，但研究结果表明，集中尿液为基础的HPV检测在预防宫颈癌方面具有相当大的前景，这是一种具有成本效益、可获得的筛查策略。

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Concentrated urine as an alternative to cervical smear samples enabling easy screening of HPV in large populations

Background

Human Papillomavirus (HPV) infection is a leading cause of cervical cancer, necessitating effective screening methods, particularly in large populations and resource limited settings. Current cervical smear-based screening faces challenges related to accessibility, invasiveness, and patient compliance. This study investigated the feasibility of using concentrated urine samples as a noninvasive alternative for HPV detection.

Methods

First-void urine samples from 126 patients were collected alongside cervical swabs. A biological fluid concentrator, MyMagiCon®, was used to concentrate the urine samples before HPV detection via RT-PCR.

Results

The results demonstrated substantial agreement (Fleiss’ kappa = 0.796, p < 0.0001) between HPV detection in concentrated urine samples and cervical smear samples. Concentrated urine samples showed a 17 % increase in HPV detection compared to unconcentrated urine.

Conclusions

This noninvasive and novel approach offers significant advantages in terms of accessibility and patient acceptance, potentially improving screening coverage and early detection rates, especially in underserved populations. Further research is needed to validate these findings in larger, more diverse populations and optimize the methodology for enhanced sensitivity and specificity, but the findings suggest concentrated urine-based HPV testing holds considerable promise as a cost-effective, accessible screening strategy in preventing cervical cancer.

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来源期刊

Journal of Infection and Public Health PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH -INFECTIOUS DISEASES

CiteScore

13.10

自引率

1.50%

发文量

203

审稿时长

96 days

期刊介绍： The Journal of Infection and Public Health, first official journal of the Saudi Arabian Ministry of National Guard Health Affairs, King Saud Bin Abdulaziz University for Health Sciences and the Saudi Association for Public Health, aims to be the foremost scientific, peer-reviewed journal encompassing infection prevention and control, microbiology, infectious diseases, public health and the application of healthcare epidemiology to the evaluation of health outcomes. The point of view of the journal is that infection and public health are closely intertwined and that advances in one area will have positive consequences on the other. The journal will be useful to all health professionals who are partners in the management of patients with communicable diseases, keeping them up to date. The journal is proud to have an international and diverse editorial board that will assist and facilitate the publication of articles that reflect a global view on infection control and public health, as well as emphasizing our focus on supporting the needs of public health practitioners. It is our aim to improve healthcare by reducing risk of infection and related adverse outcomes by critical review, selection, and dissemination of new and relevant information in the field of infection control, public health and infectious diseases in all healthcare settings and the community.