Guangmei Chen, Zhenya Tian, Yang Yue, Xuyuan Gao, Hongsong Chen, Jingfang Yang, Weihua Ma, Dehong Zheng, Huihua Tan, Zhongshi Zhou
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{"title":"共生细菌参与果胶溶解代谢,促进葫芦螯虾幼虫生长","authors":"Guangmei Chen, Zhenya Tian, Yang Yue, Xuyuan Gao, Hongsong Chen, Jingfang Yang, Weihua Ma, Dehong Zheng, Huihua Tan, Zhongshi Zhou","doi":"10.1002/ps.70035","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> BACKGROUND</h3>\n \n <p>Symbiotic microbes play a pivotal role in the feeding processes of phytophagous insects, and symbiosis has been established as a key strategy for certain species to acquire pectinases. However, whether symbiotic bacteria play a role in the pectinolytic metabolism of <i>Zeugodacus cucurbitae</i> remains unclear.</p>\n </section>\n \n <section>\n \n <h3> RESULTS</h3>\n \n <p>Removal of symbiotic bacteria <i>via</i> egg sterilization significantly reduced larval food consumption, growth, and pectinase activity (<i>P</i> < 0.05), highlighting that the microbiota was required for <i>Z. cucurbitae</i> larval growth under feeding on host plants. Microbial community analysis identified <i>Klebsiella spp.</i> as persistent colonizers of larval feeding wounds, exhibiting recycling between host tissues and plant substrates. Functional assays demonstrated that <i>Klebsiella</i> strains (CpL20, CpL49, CpL63, and CpL64) formed distinct hydrolysis zones on pectin medium and degraded pectin <i>via</i> high enzymatic activity (495.98–830.54 μ/mL). Reintroduction of <i>Klebsiella spp.</i> restored larval growth in sterile treatment groups, confirming their metabolic contribution.</p>\n </section>\n \n <section>\n \n <h3> CONCLUSION</h3>\n \n <p>Our results suggest that <i>Klebsiella spp.</i> circulate between the feeding environment and larval tissues, participating in the pectinolytic metabolism to utilize the host plant efficiently, thereby facilitating larval growth and development. This study provides a foundation for understanding the role of symbiotic bacteria in pectinolytic metabolism during the ecological adaptation of phytophagous insects and offers new insights into the environmentally friendly management of <i>Z. cucurbitae</i> in agricultural settings. © 2025 The Author(s). <i>Pest Management Science</i> published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.</p>\n </section>\n </div>","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"81 10","pages":"6820-6831"},"PeriodicalIF":3.8000,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://scijournals.onlinelibrary.wiley.com/doi/epdf/10.1002/ps.70035","citationCount":"0","resultStr":"{\"title\":\"Symbiotic bacteria participate in pectinolytic metabolism to enhance larval growth in Zeugodacus cucurbitae\",\"authors\":\"Guangmei Chen, Zhenya Tian, Yang Yue, Xuyuan Gao, Hongsong Chen, Jingfang Yang, Weihua Ma, Dehong Zheng, Huihua Tan, Zhongshi Zhou\",\"doi\":\"10.1002/ps.70035\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> BACKGROUND</h3>\\n \\n <p>Symbiotic microbes play a pivotal role in the feeding processes of phytophagous insects, and symbiosis has been established as a key strategy for certain species to acquire pectinases. However, whether symbiotic bacteria play a role in the pectinolytic metabolism of <i>Zeugodacus cucurbitae</i> remains unclear.</p>\\n </section>\\n \\n <section>\\n \\n <h3> RESULTS</h3>\\n \\n <p>Removal of symbiotic bacteria <i>via</i> egg sterilization significantly reduced larval food consumption, growth, and pectinase activity (<i>P</i> < 0.05), highlighting that the microbiota was required for <i>Z. cucurbitae</i> larval growth under feeding on host plants. Microbial community analysis identified <i>Klebsiella spp.</i> as persistent colonizers of larval feeding wounds, exhibiting recycling between host tissues and plant substrates. Functional assays demonstrated that <i>Klebsiella</i> strains (CpL20, CpL49, CpL63, and CpL64) formed distinct hydrolysis zones on pectin medium and degraded pectin <i>via</i> high enzymatic activity (495.98–830.54 μ/mL). Reintroduction of <i>Klebsiella spp.</i> restored larval growth in sterile treatment groups, confirming their metabolic contribution.</p>\\n </section>\\n \\n <section>\\n \\n <h3> CONCLUSION</h3>\\n \\n <p>Our results suggest that <i>Klebsiella spp.</i> circulate between the feeding environment and larval tissues, participating in the pectinolytic metabolism to utilize the host plant efficiently, thereby facilitating larval growth and development. This study provides a foundation for understanding the role of symbiotic bacteria in pectinolytic metabolism during the ecological adaptation of phytophagous insects and offers new insights into the environmentally friendly management of <i>Z. cucurbitae</i> in agricultural settings. © 2025 The Author(s). <i>Pest Management Science</i> published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.</p>\\n </section>\\n </div>\",\"PeriodicalId\":218,\"journal\":{\"name\":\"Pest Management Science\",\"volume\":\"81 10\",\"pages\":\"6820-6831\"},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2025-07-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://scijournals.onlinelibrary.wiley.com/doi/epdf/10.1002/ps.70035\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pest Management Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.70035\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pest Management Science","FirstCategoryId":"97","ListUrlMain":"https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.70035","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
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