共生细菌参与果胶溶解代谢,促进葫芦螯虾幼虫生长

IF 3.8 1区 农林科学 Q1 AGRONOMY
Guangmei Chen, Zhenya Tian, Yang Yue, Xuyuan Gao, Hongsong Chen, Jingfang Yang, Weihua Ma, Dehong Zheng, Huihua Tan, Zhongshi Zhou
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引用次数: 0

摘要

共生微生物在植食性昆虫的取食过程中起着至关重要的作用,共生已被确立为某些物种获取果胶酶的关键策略。然而,共生细菌是否参与葫芦丝果胶溶解代谢尚不清楚。结果通过虫卵灭菌去除共生细菌可显著降低幼虫的食用量、生长和果胶酶活性(P <;0.05),说明在取食寄主植物的条件下,葫芦小蠹幼虫的生长需要一定的菌群。微生物群落分析发现克雷伯氏菌是幼虫取食伤口的持久定植者,表现出寄主组织和植物基质之间的循环。功能分析表明,克雷伯菌(CpL20、CpL49、CpL63和CpL64)在果胶培养基上形成明显的水解区,酶活性较高(495.98 ~ 830.54 μ/mL)。在无菌处理组中,重新引入克雷伯氏菌恢复了幼虫的生长,证实了它们的代谢贡献。结论克雷伯氏菌在取食环境和幼虫组织之间循环,参与果胶溶解代谢,有效利用寄主植物,促进幼虫生长发育。本研究为了解共生细菌在植食性昆虫生态适应过程中果胶分解代谢中的作用奠定了基础,并为农业环境下葫芦丝的环境友好管理提供了新的见解。©2025作者。《病虫害管理科学》由John Wiley &出版;我代表化学工业协会的儿子有限公司。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Symbiotic bacteria participate in pectinolytic metabolism to enhance larval growth in Zeugodacus cucurbitae

Symbiotic bacteria participate in pectinolytic metabolism to enhance larval growth in Zeugodacus cucurbitae

Symbiotic bacteria participate in pectinolytic metabolism to enhance larval growth in Zeugodacus cucurbitae

Symbiotic bacteria participate in pectinolytic metabolism to enhance larval growth in Zeugodacus cucurbitae

BACKGROUND

Symbiotic microbes play a pivotal role in the feeding processes of phytophagous insects, and symbiosis has been established as a key strategy for certain species to acquire pectinases. However, whether symbiotic bacteria play a role in the pectinolytic metabolism of Zeugodacus cucurbitae remains unclear.

RESULTS

Removal of symbiotic bacteria via egg sterilization significantly reduced larval food consumption, growth, and pectinase activity (P < 0.05), highlighting that the microbiota was required for Z. cucurbitae larval growth under feeding on host plants. Microbial community analysis identified Klebsiella spp. as persistent colonizers of larval feeding wounds, exhibiting recycling between host tissues and plant substrates. Functional assays demonstrated that Klebsiella strains (CpL20, CpL49, CpL63, and CpL64) formed distinct hydrolysis zones on pectin medium and degraded pectin via high enzymatic activity (495.98–830.54 μ/mL). Reintroduction of Klebsiella spp. restored larval growth in sterile treatment groups, confirming their metabolic contribution.

CONCLUSION

Our results suggest that Klebsiella spp. circulate between the feeding environment and larval tissues, participating in the pectinolytic metabolism to utilize the host plant efficiently, thereby facilitating larval growth and development. This study provides a foundation for understanding the role of symbiotic bacteria in pectinolytic metabolism during the ecological adaptation of phytophagous insects and offers new insights into the environmentally friendly management of Z. cucurbitae in agricultural settings. © 2025 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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来源期刊
Pest Management Science
Pest Management Science 农林科学-昆虫学
CiteScore
7.90
自引率
9.80%
发文量
553
审稿时长
4.8 months
期刊介绍: Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.
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