Anthony E Felder, Giri Balasubramanian, Aldo Arroyo, Jason C Park, Mahnaz Shahidi, J Jason McAnany
{"title":"一种评估视网膜血管和神经指标对刺激调制频率依赖性的方法。","authors":"Anthony E Felder, Giri Balasubramanian, Aldo Arroyo, Jason C Park, Mahnaz Shahidi, J Jason McAnany","doi":"10.1167/tvst.14.7.3","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>Flickering light stimulation induces functional hyperemia, characterized by vasodilation, blood flow augmentation, and venous oxygen elevation. We present a new method to investigate the frequency dependence of metrics associated with functional hyperemia.</p><p><strong>Methods: </strong>A novel optical imaging system was developed to quantify retinal blood vessel diameter (D), oxygen saturation (SO2), and the inner retinal oxygen extraction fraction (OEF) before and after light flicker at different frequencies. Measurements were performed in 10 visually normal subjects (20-62 years) at flicker frequencies from 2 to 30 Hz. In addition, a measure of neural function was obtained by steady-state pattern electroretinography (ssPERG) across a similar range of frequencies.</p><p><strong>Results: </strong>Flicker stimulation greater than 2 Hz increased D, increased SO2 in veins, and decreased OEF. The maximum response for all metrics was obtained between 16 and 30 Hz, indicating that vascular and oxygenation metrics share a similar frequency response with light flicker. ssPERG amplitudes were positively correlated with flicker-induced increases in venous D and SO2. ssPERG amplitude was negatively correlated with flicker-induced decreases in OEF.</p><p><strong>Conclusions: </strong>We present a novel retinal imaging method to evaluate the frequency dependence of changes in D, SO2, and OEF to light flicker stimulation. The relationship between these metrics and ssPERG amplitudes was evaluated.</p><p><strong>Translational relevance: </strong>The frequency-dependent response of retinal D, SO2, and OEF established in healthy individuals herein has the potential to serve as a biomarker of vascular and tissue abnormality in future studies of retinal disease.</p>","PeriodicalId":23322,"journal":{"name":"Translational Vision Science & Technology","volume":"14 7","pages":"3"},"PeriodicalIF":2.6000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12227032/pdf/","citationCount":"0","resultStr":"{\"title\":\"A Method to Evaluate the Frequency Dependence of Retinal Vascular and Neural Metrics to Stimulus Modulation.\",\"authors\":\"Anthony E Felder, Giri Balasubramanian, Aldo Arroyo, Jason C Park, Mahnaz Shahidi, J Jason McAnany\",\"doi\":\"10.1167/tvst.14.7.3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>Flickering light stimulation induces functional hyperemia, characterized by vasodilation, blood flow augmentation, and venous oxygen elevation. We present a new method to investigate the frequency dependence of metrics associated with functional hyperemia.</p><p><strong>Methods: </strong>A novel optical imaging system was developed to quantify retinal blood vessel diameter (D), oxygen saturation (SO2), and the inner retinal oxygen extraction fraction (OEF) before and after light flicker at different frequencies. Measurements were performed in 10 visually normal subjects (20-62 years) at flicker frequencies from 2 to 30 Hz. In addition, a measure of neural function was obtained by steady-state pattern electroretinography (ssPERG) across a similar range of frequencies.</p><p><strong>Results: </strong>Flicker stimulation greater than 2 Hz increased D, increased SO2 in veins, and decreased OEF. The maximum response for all metrics was obtained between 16 and 30 Hz, indicating that vascular and oxygenation metrics share a similar frequency response with light flicker. ssPERG amplitudes were positively correlated with flicker-induced increases in venous D and SO2. ssPERG amplitude was negatively correlated with flicker-induced decreases in OEF.</p><p><strong>Conclusions: </strong>We present a novel retinal imaging method to evaluate the frequency dependence of changes in D, SO2, and OEF to light flicker stimulation. 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A Method to Evaluate the Frequency Dependence of Retinal Vascular and Neural Metrics to Stimulus Modulation.
Purpose: Flickering light stimulation induces functional hyperemia, characterized by vasodilation, blood flow augmentation, and venous oxygen elevation. We present a new method to investigate the frequency dependence of metrics associated with functional hyperemia.
Methods: A novel optical imaging system was developed to quantify retinal blood vessel diameter (D), oxygen saturation (SO2), and the inner retinal oxygen extraction fraction (OEF) before and after light flicker at different frequencies. Measurements were performed in 10 visually normal subjects (20-62 years) at flicker frequencies from 2 to 30 Hz. In addition, a measure of neural function was obtained by steady-state pattern electroretinography (ssPERG) across a similar range of frequencies.
Results: Flicker stimulation greater than 2 Hz increased D, increased SO2 in veins, and decreased OEF. The maximum response for all metrics was obtained between 16 and 30 Hz, indicating that vascular and oxygenation metrics share a similar frequency response with light flicker. ssPERG amplitudes were positively correlated with flicker-induced increases in venous D and SO2. ssPERG amplitude was negatively correlated with flicker-induced decreases in OEF.
Conclusions: We present a novel retinal imaging method to evaluate the frequency dependence of changes in D, SO2, and OEF to light flicker stimulation. The relationship between these metrics and ssPERG amplitudes was evaluated.
Translational relevance: The frequency-dependent response of retinal D, SO2, and OEF established in healthy individuals herein has the potential to serve as a biomarker of vascular and tissue abnormality in future studies of retinal disease.
期刊介绍：
Translational Vision Science & Technology (TVST), an official journal of the Association for Research in Vision and Ophthalmology (ARVO), an international organization whose purpose is to advance research worldwide into understanding the visual system and preventing, treating and curing its disorders, is an online, open access, peer-reviewed journal emphasizing multidisciplinary research that bridges the gap between basic research and clinical care. A highly qualified and diverse group of Associate Editors and Editorial Board Members is led by Editor-in-Chief Marco Zarbin, MD, PhD, FARVO.
The journal covers a broad spectrum of work, including but not limited to:
Applications of stem cell technology for regenerative medicine,
Development of new animal models of human diseases,
Tissue bioengineering,
Chemical engineering to improve virus-based gene delivery,
Nanotechnology for drug delivery,
Design and synthesis of artificial extracellular matrices,
Development of a true microsurgical operating environment,
Refining data analysis algorithms to improve in vivo imaging technology,
Results of Phase 1 clinical trials,
Reverse translational ("bedside to bench") research.
TVST seeks manuscripts from scientists and clinicians with diverse backgrounds ranging from basic chemistry to ophthalmic surgery that will advance or change the way we understand and/or treat vision-threatening diseases. TVST encourages the use of color, multimedia, hyperlinks, program code and other digital enhancements.
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