铝乳混合纳米颗粒与TLR9激动剂构建的新型佐剂递送体系增强了疫苗免疫力。

IF 10.6 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Yarong Zeng, Yifan Yin, Jialin Xu, Rong Su, Sibo Zhang, Feng Han, Yufang Li, Xueqing Zhu, Ciying Qian, Feihong Zou, Renfei Gao, Xiuli Zhang, Lizhi Zhou, Tingting Li, Min Lin, Qingbing Zheng, Hai Yu, Jun Zhang, Zizheng Zheng, Ying Gu, Ningshao Xia, Shaowei Li
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引用次数: 0

摘要

疫苗佐剂递送系统(VADSs)结合了递送和佐剂是现代疫苗研究的重要策略。在这里,我们使用微流体将纳米氢氧化铝(XA)和纳米乳液(XE)以不同的比例混合。一种2:3 (v/v)的混合物产生了约220 nm的纳米颗粒(XAE),具有接近中性的zeta电位,显著增强了DC2.4细胞的抗原摄取,并显示出比单独XA或XE更强的佐剂活性。通过静电吸附TLR9激动剂CpG到XAE上,我们构建了XAEC作为一个集成的递送平台,共同递送抗原和免疫刺激分子,从而提高CpG的生物利用度和生物相容性。这种XAEC平台在注射部位产生了一个“免疫激活的微环境”,促进抗原提呈细胞(APC)募集,增强抗原提呈,促进疫苗运输到免疫器官,并引发th1偏向性免疫反应。XAEC显著提高了呼吸道合胞病毒(RSV)和水痘带状疱疹病毒(VZV)预防疫苗的免疫原性,并在人乳头瘤病毒(HPV)肿瘤模型中诱导了有效的抗肿瘤反应。因此,XAEC作为一种有前景的新一代疫苗佐剂系统出现。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Novel adjuvant delivery system constructed by alum-emulsion hybrid nanoparticles with TLR9 agonists boosts vaccine immunity.

Vaccine Adjuvant Delivery Systems (VADSs) that integrate both delivery and adjuvancy constitute a vital strategy in modern vaccine research. Herein, we employed microfluidics to blend nanosized aluminum hydroxide (XA) and a nanoemulsion (XE) in varying proportions. A 2:3 (v/v) mixture produced nanoparticles (XAE) of approximately 220 nm with near-neutral zeta potential, which significantly enhanced antigen uptake in DC2.4 cells and displayed stronger adjuvant activity than XA or XE alone. By electrostatically adsorbing the TLR9 agonist CpG onto XAE, we constructed XAEC as an integrated delivery platform that co-delivers antigens and immune-stimulatory molecules, thereby improving CpG's bioavailability and biocompatibility. This XAEC platform generated an "immune-activated microenvironment" at the injection site, fostering antigen-presenting cell (APC) recruitment, augmenting antigen presentation, facilitating vaccine trafficking to immune organs, and eliciting a Th1-biased immune response. XAEC substantially boosted immunogenicity in prophylactic Respiratory Syncytial Virus (RSV) and Varicella-Zoster Virus (VZV) vaccines and induced potent antitumor responses in a Human Papillomavirus (HPV) tumor model. Hence, XAEC emerges as a promising next-generation vaccine adjuvant system.

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来源期刊
Journal of Nanobiotechnology
Journal of Nanobiotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-NANOSCIENCE & NANOTECHNOLOGY
CiteScore
13.90
自引率
4.90%
发文量
493
审稿时长
16 weeks
期刊介绍: Journal of Nanobiotechnology is an open access peer-reviewed journal communicating scientific and technological advances in the fields of medicine and biology, with an emphasis in their interface with nanoscale sciences. The journal provides biomedical scientists and the international biotechnology business community with the latest developments in the growing field of Nanobiotechnology.
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