PTEN抑制通过AKT信号通路诱导SH-SY5Y细胞的神经元分化和神经细胞发生。

IF 3.4 3区 医学 Q2 NEUROSCIENCES
Natália Chermont Dos Santos Moreira, Larissa de Oliveira Piassi, Jéssica Ellen Barbosa de Freitas Lima, Geraldo Aleixo Passos, Elza Tiemi Sakamoto-Hojo
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引用次数: 0

摘要

pten是神经元分化和神经发生的关键调节因子。在神经元模型中,它在调节PI3K/AKT通路和氧化应激反应中的作用仍然是一个积极研究的领域。目的探讨PTEN基因下调对SH-SY5Y细胞神经元分化、神经炎生长和PI3K/AKT通路激活的影响。方法用PTEN siRNA处理sh - sy5y细胞,诱导PTEN表达下调。确认PTEN抑制水平,并在治疗后3天和7天进行神经发生和神经细胞发生测定。对AKT/GSK3-β/Tau通路关键组分进行蛋白表达分析,以评估其在神经发生中的作用。此外,使用PI3K抑制剂LY294002来检测其对PTEN敲低诱导的神经元分化的影响。结果spten基因敲除后,神经突长度明显增加,胞质大小明显减小,提示神经元分化。蛋白分析显示,PTEN抑制可调节AKT/GSK3-β/Tau通路组分的表达。PI3K抑制剂LY294002阻止神经元分化,证实PI3K/AKT通路参与介导PTEN下调的作用。结论PTEN在SH-SY5Y细胞的神经元分化调控中起重要作用。PI3K/AKT通路介导PTEN敲低的作用,提示PTEN是神经退行性疾病的潜在治疗靶点,其失调可能导致疾病进展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
PTEN inhibition induces neuronal differentiation and neuritogenesis in SH-SY5Y cells via AKT signaling pathway.

BackgroundPTEN is a key regulator of neuronal differentiation and neurogenesis. Its role in modulating the PI3K/AKT pathway and oxidative stress responses in neuronal models remains an area of active investigation.ObjectiveThis study aimed to assess the effects of PTEN knockdown on neuronal differentiation, neuritic growth, and PI3K/AKT pathway activation in SH-SY5Y cells.MethodsSH-SY5Y cells were treated with PTEN siRNA to induce PTEN knockdown. The level of PTEN inhibition was confirmed, and assays were performed to evaluate neurogenesis and neuritogenesis at 3- and 7-days post-treatment. Protein expression analysis of key components in the AKT/GSK3-β/Tau pathway was conducted to assess their role in neurogenesis. Additionally, the PI3K inhibitor LY294002 was used to examine its impact on PTEN knockdown-induced neuronal differentiation.ResultsPTEN knockdown significantly increased neurite lengths and reduced cytoplasmic size, indicating neuronal differentiation. Protein analysis showed that PTEN inhibition modulated the expression of components in the AKT/GSK3-β/Tau pathway. The PI3K inhibitor LY294002 prevented neuronal differentiation, confirming the involvement of the PI3K/AKT pathway in mediating the effects of PTEN knockdown.ConclusionsOur findings demonstrate that PTEN plays a crucial role in regulating neuronal differentiation in SH-SY5Y cells. The PI3K/AKT pathway mediates the effects of PTEN knockdown, suggesting PTEN as a potential therapeutic target for neurodegenerative diseases where its dysregulation may contribute to disease progression.

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来源期刊
Journal of Alzheimer's Disease
Journal of Alzheimer's Disease 医学-神经科学
CiteScore
6.40
自引率
7.50%
发文量
1327
审稿时长
2 months
期刊介绍: The Journal of Alzheimer''s Disease (JAD) is an international multidisciplinary journal to facilitate progress in understanding the etiology, pathogenesis, epidemiology, genetics, behavior, treatment and psychology of Alzheimer''s disease. The journal publishes research reports, reviews, short communications, hypotheses, ethics reviews, book reviews, and letters-to-the-editor. The journal is dedicated to providing an open forum for original research that will expedite our fundamental understanding of Alzheimer''s disease.
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