短螺旋体影响传统和有机生产体系下蛋鸡肠道免疫标记物的转录调节。

IF 1.7 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

British Poultry Science Pub Date : 2025-07-02 DOI:10.1080/00071668.2025.2517293

N N Arechavaleta, G M Breyer, F M Siqueira

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引用次数: 0

摘要

1. 本研究从传统和有机生产体系中鉴定出了产蛋母鸡的中间短螺旋体和毛毛杆菌病原体，并研究了它们对肠道免疫标志物转录调控的影响。采用qPCR方法对两种生产系统的粪便和鸡蛋样本进行了中间芽孢杆菌和毛囊双歧杆菌的分子鉴定。采用rt - qpcr技术研究9种肠道免疫应答标志物在盲肠组织中的相对基因表达。33%（13/40）的粪便样本中检出短螺旋体，但卵中未检出。常规母鸡的检出率为25%(5/20)，有机母鸡的检出率为40%（8/20）。含短螺旋体的蛋鸡IL-4水平上调，IL-4是调节抗体产生、造血和炎症的重要细胞因子。总体而言，在家禽中发现中间芽胞杆菌和大肠杆菌有助于实施预防措施，最大限度地减少家禽养殖场的经济损失，并降低人畜共患病传播的风险。

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Brachyspira spp. influences transcriptional regulation of intestinal immune markers in laying hens from conventional and organic production systems.

1. This study identified the pathogen Brachyspira intermedia and B. pilosicoli in laying hens from conventional and organic production systems and investigated their effects on the transcriptional regulation of intestinal immune markers.2. Molecular identification of B. intermedia and B. pilosicoli was performed by qPCR in faecal and egg samples of both production systems. The relative gene expression of nine intestinal immune response markers in caecal tissue was investigated using RT-qPCR.3. Brachyspira spp. was detected in 33% (13/40) of faecal samples but not in eggs. In conventional hens, detection was 25% (5/20) and in organic hens 40% (8/20). Laying hens harbouring Brachyspira spp. showed upregulation of IL-4, important for cytokines regulating antibody production, haematopoiesis and inflammation.4. Overall, identifying B. intermedia and B. pilosicoli in poultry helps implement preventive measures to minimise economic losses in poultry farms and reduce the risk of zoonotic transmission.

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来源期刊

British Poultry Science 农林科学-奶制品与动物科学

CiteScore

3.90

自引率

5.00%

发文量

审稿时长

4.5 months

期刊介绍： From its first volume in 1960, British Poultry Science has been a leading international journal for poultry scientists and advisers to the poultry industry throughout the world. Over 60% of the independently refereed papers published originate outside the UK. Most typically they report the results of biological studies with an experimental approach which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: anatomy, embryology, biochemistry, biophysics, physiology, reproduction and genetics, behaviour, microbiology, endocrinology, nutrition, environmental science, food science, feeding stuffs and feeding, management and housing welfare, breeding, hatching, poultry meat and egg yields and quality.Papers that adopt a modelling approach or describe the scientific background to new equipment or apparatus directly relevant to the industry are also published. The journal also features rapid publication of Short Communications. Summaries of papers presented at the Spring Meeting of the UK Branch of the WPSA are published in British Poultry Abstracts .