水通道蛋白9通过PP2A/NF-κB通路促进lps诱导的耳咽管炎症。

IF 8.5 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules Pub Date : 2025-09-01 Epub Date: 2025-06-29 DOI:10.1016/j.ijbiomac.2025.145655

Rui Li, Xuan Fang, Huaicun Liu, Yueqi Wang, Zhen Zhong, Junxiao Jia, Yu Song, Quancheng Cheng, Man Li, Chao Wang, Weiguang Zhang, Junxiu Liu

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引用次数: 0

摘要

耳咽管炎症引起的耳咽管功能障碍是导致中耳积液和听力损害的重要原因。在这里，我们发现水通道蛋白9 （AQP9）是ET炎症的重要调节因子。在ET炎症过程中，AQP9表达上调，而AQP9缺乏通过上调蛋白磷酸酶2A （PP2A）表达，通过P65磷酸化抑制核因子κ b （NF-κB）活化，从而减轻lps诱导的ET炎症。此外，根皮素对AQP9的药理抑制同样减轻了ET炎症。这些发现为治疗ET炎症以改善ETD提供了新的靶点。

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Aquaporin 9 promotes LPS-induced Eustachian tube inflammation through PP2A/NF-κB pathway.

Eustachian tube dysfunction (ETD) induced by Eustachian tube (ET) inflammation is an important cause to middle ear effusion and hearing impairment. Here, we found that aquaporin 9 (AQP9) was an important regulator of ET inflammation. AQP9 was upregulated during ET inflammation, while AQP9 deficiency alleviated LPS-induced ET inflammation by upregulating protein phosphatase 2A (PP2A) expression to suppress nuclear factor kappa-B (NF-κB) activation through P65 phosphorylation. In addition, pharmacological inhibition of AQP9 by phloretin similarly alleviated ET inflammation. These findings provide a new target for the treatment of ET inflammation to improve ETD.

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来源期刊

International Journal of Biological Macromolecules 生物-生化与分子生物学

CiteScore

13.70

自引率

9.80%

发文量

2728

审稿时长

64 days

期刊介绍： The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.