Ha Thi Phuong, Tran Thu Huong, Le Thi Vinh, Do Thi Thao, Le Anh Tu, Tong Quang Cong, Nguyen Duc Van and Tran Quoc Tien
{"title":"SCN-IgG功能化的NaYF4:Yb3+/(Er3+,Tm3+)上转换纳米颗粒用于肝癌细胞的靶向荧光成像","authors":"Ha Thi Phuong, Tran Thu Huong, Le Thi Vinh, Do Thi Thao, Le Anh Tu, Tong Quang Cong, Nguyen Duc Van and Tran Quoc Tien","doi":"10.1039/D5RA03912F","DOIUrl":null,"url":null,"abstract":"<p >Lanthanide-doped upconversion nanoparticles (UCNPs) offer significant potential for bioimaging due to their ability to convert near-infrared (NIR) excitation into visible emission. In this study, NaYF<small><sub>4</sub></small>:Yb<small><sup>3+</sup></small>/(Er<small><sup>3+</sup></small>,Tm<small><sup>3+</sup></small>) UCNPs were synthesized <em>via</em> a hydrothermal method and sequentially functionalized with a silica shell, amine, thiocyanate (SCN), and immunoglobulin G (IgG) to enhance their biocompatibility and targeting capabilities. Structural characterization confirmed the formation of highly crystalline β-NaYF<small><sub>4</sub></small> cores with uniform morphology and successful surface modification. The functionalized nanoparticles exhibited strong upconversion luminescence under 980 nm excitation, with multicolor emission dominated by red light (∼660 nm). Fluorescence microscopy and flow cytometry demonstrated selective labeling of HepG2 liver cancer cells, achieving a labeling efficiency of 18.10% for SCN–IgG-conjugated nanoparticles—significantly higher than in unmodified controls. These findings demonstrate that SCN–IgG-functionalized UCNPs are effective and selective nanoprobes for targeted fluorescence imaging and hold promise for the early diagnosis of liver cancer.</p>","PeriodicalId":102,"journal":{"name":"RSC Advances","volume":" 28","pages":" 22682-22689"},"PeriodicalIF":4.6000,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/ra/d5ra03912f?page=search","citationCount":"0","resultStr":"{\"title\":\"SCN–IgG functionalized NaYF4:Yb3+/(Er3+,Tm3+) upconversion nanoparticles for targeted fluorescence imaging of liver cancer cells\",\"authors\":\"Ha Thi Phuong, Tran Thu Huong, Le Thi Vinh, Do Thi Thao, Le Anh Tu, Tong Quang Cong, Nguyen Duc Van and Tran Quoc Tien\",\"doi\":\"10.1039/D5RA03912F\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Lanthanide-doped upconversion nanoparticles (UCNPs) offer significant potential for bioimaging due to their ability to convert near-infrared (NIR) excitation into visible emission. In this study, NaYF<small><sub>4</sub></small>:Yb<small><sup>3+</sup></small>/(Er<small><sup>3+</sup></small>,Tm<small><sup>3+</sup></small>) UCNPs were synthesized <em>via</em> a hydrothermal method and sequentially functionalized with a silica shell, amine, thiocyanate (SCN), and immunoglobulin G (IgG) to enhance their biocompatibility and targeting capabilities. Structural characterization confirmed the formation of highly crystalline β-NaYF<small><sub>4</sub></small> cores with uniform morphology and successful surface modification. The functionalized nanoparticles exhibited strong upconversion luminescence under 980 nm excitation, with multicolor emission dominated by red light (∼660 nm). Fluorescence microscopy and flow cytometry demonstrated selective labeling of HepG2 liver cancer cells, achieving a labeling efficiency of 18.10% for SCN–IgG-conjugated nanoparticles—significantly higher than in unmodified controls. These findings demonstrate that SCN–IgG-functionalized UCNPs are effective and selective nanoprobes for targeted fluorescence imaging and hold promise for the early diagnosis of liver cancer.</p>\",\"PeriodicalId\":102,\"journal\":{\"name\":\"RSC Advances\",\"volume\":\" 28\",\"pages\":\" 22682-22689\"},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2025-07-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://pubs.rsc.org/en/content/articlepdf/2025/ra/d5ra03912f?page=search\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"RSC Advances\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2025/ra/d5ra03912f\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"RSC Advances","FirstCategoryId":"92","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2025/ra/d5ra03912f","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
SCN–IgG functionalized NaYF4:Yb3+/(Er3+,Tm3+) upconversion nanoparticles for targeted fluorescence imaging of liver cancer cells
Lanthanide-doped upconversion nanoparticles (UCNPs) offer significant potential for bioimaging due to their ability to convert near-infrared (NIR) excitation into visible emission. In this study, NaYF4:Yb3+/(Er3+,Tm3+) UCNPs were synthesized via a hydrothermal method and sequentially functionalized with a silica shell, amine, thiocyanate (SCN), and immunoglobulin G (IgG) to enhance their biocompatibility and targeting capabilities. Structural characterization confirmed the formation of highly crystalline β-NaYF4 cores with uniform morphology and successful surface modification. The functionalized nanoparticles exhibited strong upconversion luminescence under 980 nm excitation, with multicolor emission dominated by red light (∼660 nm). Fluorescence microscopy and flow cytometry demonstrated selective labeling of HepG2 liver cancer cells, achieving a labeling efficiency of 18.10% for SCN–IgG-conjugated nanoparticles—significantly higher than in unmodified controls. These findings demonstrate that SCN–IgG-functionalized UCNPs are effective and selective nanoprobes for targeted fluorescence imaging and hold promise for the early diagnosis of liver cancer.
期刊介绍:
An international, peer-reviewed journal covering all of the chemical sciences, including multidisciplinary and emerging areas. RSC Advances is a gold open access journal allowing researchers free access to research articles, and offering an affordable open access publishing option for authors around the world.