SCN-IgG功能化的NaYF4:Yb3+/(Er3+,Tm3+)上转换纳米颗粒用于肝癌细胞的靶向荧光成像

IF 4.6 3区 化学 Q2 CHEMISTRY, MULTIDISCIPLINARY
RSC Advances Pub Date : 2025-07-02 DOI:10.1039/D5RA03912F
Ha Thi Phuong, Tran Thu Huong, Le Thi Vinh, Do Thi Thao, Le Anh Tu, Tong Quang Cong, Nguyen Duc Van and Tran Quoc Tien
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引用次数: 0

摘要

镧系元素掺杂的上转换纳米粒子(UCNPs)具有将近红外(NIR)激发转化为可见光发射的能力,为生物成像提供了巨大的潜力。本研究通过水热法合成了NaYF4:Yb3+/(Er3+,Tm3+) UCNPs,并分别用硅壳、胺、硫氰酸酯(SCN)和免疫球蛋白G (IgG)进行了功能化,以增强其生物相容性和靶向性。结构表征证实了高结晶β-NaYF4核的形成,具有均匀的形貌和成功的表面改性。功能化纳米粒子在980 nm激发下表现出强上转换发光,以红光(~ 660 nm)为主的多色发光。荧光显微镜和流式细胞术显示了HepG2肝癌细胞的选择性标记,scn - igg共轭纳米颗粒的标记效率为18.10%,显著高于未修饰的对照组。这些发现表明,scn - igg功能化的UCNPs是一种有效的、选择性的靶向荧光成像纳米探针,有望用于肝癌的早期诊断。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

SCN–IgG functionalized NaYF4:Yb3+/(Er3+,Tm3+) upconversion nanoparticles for targeted fluorescence imaging of liver cancer cells

SCN–IgG functionalized NaYF4:Yb3+/(Er3+,Tm3+) upconversion nanoparticles for targeted fluorescence imaging of liver cancer cells

Lanthanide-doped upconversion nanoparticles (UCNPs) offer significant potential for bioimaging due to their ability to convert near-infrared (NIR) excitation into visible emission. In this study, NaYF4:Yb3+/(Er3+,Tm3+) UCNPs were synthesized via a hydrothermal method and sequentially functionalized with a silica shell, amine, thiocyanate (SCN), and immunoglobulin G (IgG) to enhance their biocompatibility and targeting capabilities. Structural characterization confirmed the formation of highly crystalline β-NaYF4 cores with uniform morphology and successful surface modification. The functionalized nanoparticles exhibited strong upconversion luminescence under 980 nm excitation, with multicolor emission dominated by red light (∼660 nm). Fluorescence microscopy and flow cytometry demonstrated selective labeling of HepG2 liver cancer cells, achieving a labeling efficiency of 18.10% for SCN–IgG-conjugated nanoparticles—significantly higher than in unmodified controls. These findings demonstrate that SCN–IgG-functionalized UCNPs are effective and selective nanoprobes for targeted fluorescence imaging and hold promise for the early diagnosis of liver cancer.

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来源期刊
RSC Advances
RSC Advances chemical sciences-
CiteScore
7.50
自引率
2.60%
发文量
3116
审稿时长
1.6 months
期刊介绍: An international, peer-reviewed journal covering all of the chemical sciences, including multidisciplinary and emerging areas. RSC Advances is a gold open access journal allowing researchers free access to research articles, and offering an affordable open access publishing option for authors around the world.
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