{"title":"乳清素与香茅醛对玉米镰刀菌的协同作用及其在玉米保鲜中的应用","authors":"Shiqi He, Yingxin Wei, Zhanyi Yang, Licong Zhang, Anshan Shan","doi":"10.1016/j.ijfoodmicro.2025.111331","DOIUrl":null,"url":null,"abstract":"<div><div><em>Fusarium graminearum</em> (<em>F. graminearum</em>), responsible for Fusarium head blight in cereals, causes significant yield losses and produces mycotoxins contaminating food and feed. The combined effects of nisin and citronellal against <em>F. graminearum</em> were investigated in this study. The combination of nisin and citronellal not only exhibited a synergistic effect against <em>F. graminearum</em> spores (FICI <0.3125), but also significantly inhibited mycelial growth, with inhibition rates of (76.93 ± 6.74) % and (95.24 ± 4.33) % for mycelial diameter and weight, respectively. The nisin/citronellal combination maintained stable antifungal activity independent of salts, high temperature, and acid–base conditions. Mechanistically, citronellal strongly inhibited energy metabolism, nisin significantly reduced the ergosterol content (to our knowledge, the first report of nisin suppressing ergosterol in <em>F. graminearum</em>), and the combination of citronellal and nisin further enhanced disruption of fungal cell membranes. Moreover, due to the reduced dosage and involvement of multiple mechanisms, the nisin/citronellal combination did not induce resistance in <em>F. graminearum</em> after 30 generations, with MIC fold changes of 0.25 to 2. Finally, the combination effectively suppressed fungal contamination in maize. Overall, nisin and citronellal could be used together for controlling <em>F. graminearum</em> in food and feed industries. Although the production costs of nisin and citronellal remain higher than those of synthetic fungicides, the continuous development of biotechnology is driving their application in post-harvest grain storage.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"441 ","pages":"Article 111331"},"PeriodicalIF":5.0000,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Synergism between nisin and citronellal against Fusarium graminearum and their application in maize preservation\",\"authors\":\"Shiqi He, Yingxin Wei, Zhanyi Yang, Licong Zhang, Anshan Shan\",\"doi\":\"10.1016/j.ijfoodmicro.2025.111331\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div><em>Fusarium graminearum</em> (<em>F. graminearum</em>), responsible for Fusarium head blight in cereals, causes significant yield losses and produces mycotoxins contaminating food and feed. The combined effects of nisin and citronellal against <em>F. graminearum</em> were investigated in this study. The combination of nisin and citronellal not only exhibited a synergistic effect against <em>F. graminearum</em> spores (FICI <0.3125), but also significantly inhibited mycelial growth, with inhibition rates of (76.93 ± 6.74) % and (95.24 ± 4.33) % for mycelial diameter and weight, respectively. The nisin/citronellal combination maintained stable antifungal activity independent of salts, high temperature, and acid–base conditions. Mechanistically, citronellal strongly inhibited energy metabolism, nisin significantly reduced the ergosterol content (to our knowledge, the first report of nisin suppressing ergosterol in <em>F. graminearum</em>), and the combination of citronellal and nisin further enhanced disruption of fungal cell membranes. Moreover, due to the reduced dosage and involvement of multiple mechanisms, the nisin/citronellal combination did not induce resistance in <em>F. graminearum</em> after 30 generations, with MIC fold changes of 0.25 to 2. Finally, the combination effectively suppressed fungal contamination in maize. Overall, nisin and citronellal could be used together for controlling <em>F. graminearum</em> in food and feed industries. Although the production costs of nisin and citronellal remain higher than those of synthetic fungicides, the continuous development of biotechnology is driving their application in post-harvest grain storage.</div></div>\",\"PeriodicalId\":14095,\"journal\":{\"name\":\"International journal of food microbiology\",\"volume\":\"441 \",\"pages\":\"Article 111331\"},\"PeriodicalIF\":5.0000,\"publicationDate\":\"2025-06-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International journal of food microbiology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0168160525002764\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of food microbiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0168160525002764","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
Synergism between nisin and citronellal against Fusarium graminearum and their application in maize preservation
Fusarium graminearum (F. graminearum), responsible for Fusarium head blight in cereals, causes significant yield losses and produces mycotoxins contaminating food and feed. The combined effects of nisin and citronellal against F. graminearum were investigated in this study. The combination of nisin and citronellal not only exhibited a synergistic effect against F. graminearum spores (FICI <0.3125), but also significantly inhibited mycelial growth, with inhibition rates of (76.93 ± 6.74) % and (95.24 ± 4.33) % for mycelial diameter and weight, respectively. The nisin/citronellal combination maintained stable antifungal activity independent of salts, high temperature, and acid–base conditions. Mechanistically, citronellal strongly inhibited energy metabolism, nisin significantly reduced the ergosterol content (to our knowledge, the first report of nisin suppressing ergosterol in F. graminearum), and the combination of citronellal and nisin further enhanced disruption of fungal cell membranes. Moreover, due to the reduced dosage and involvement of multiple mechanisms, the nisin/citronellal combination did not induce resistance in F. graminearum after 30 generations, with MIC fold changes of 0.25 to 2. Finally, the combination effectively suppressed fungal contamination in maize. Overall, nisin and citronellal could be used together for controlling F. graminearum in food and feed industries. Although the production costs of nisin and citronellal remain higher than those of synthetic fungicides, the continuous development of biotechnology is driving their application in post-harvest grain storage.
期刊介绍:
The International Journal of Food Microbiology publishes papers dealing with all aspects of food microbiology. Articles must present information that is novel, has high impact and interest, and is of high scientific quality. They should provide scientific or technological advancement in the specific field of interest of the journal and enhance its strong international reputation. Preliminary or confirmatory results as well as contributions not strictly related to food microbiology will not be considered for publication.