Akriti Dhungana, Daniel M McCalley, Alesha M Heath, Eric P Kraybill, Fatemeh S Mojabi, Jairelisse Morales Morales, Allison R Morningstar, Allyson K Davis, Claudia B Padula, William J Giardino, M Windy McNerney
{"title":"在酒精使用障碍中建立低强度rTMS的反向翻译模型：θ波爆发刺激方案对小鼠暴饮的影响。","authors":"Akriti Dhungana, Daniel M McCalley, Alesha M Heath, Eric P Kraybill, Fatemeh S Mojabi, Jairelisse Morales Morales, Allison R Morningstar, Allyson K Davis, Claudia B Padula, William J Giardino, M Windy McNerney","doi":"10.1016/j.transm.2025.100098","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Repetitive Transcranial Magnetic Stimulation (rTMS) is a promising treatment tool for Alcohol Use Disorder (AUD). A challenge facing the field is that the optimal TMS parameters to reduce drinking are unknown. There are now rodent TMS coils which can be adapted to evaluate rTMS-induced changes in alcohol consumption in a rapid, cost-effective manner.</p><p><strong>Objective: </strong>Develop a preclinical model of rTMS in alcohol consuming rodents and collect pilot data to evaluate the influence rTMS parameters (here, theta burst pattern) on change in alcohol consumption and biochemistry.</p><p><strong>Methods: </strong>66 C57BL/6 J mice (32 F) received sham, intermittent Theta Burst Stimulation (iTBS), or continuous Theta Burst Stimulation (cTBS) (14 sessions, 2 sessions/day, low intensity 16mT stimulation). Alcohol consumption and preference were evaluated before and after rTMS using a two-bottle choice, Drinking in the Dark (DID) paradigm. Cortical brain tissue was assayed for BDNF gene expression via qPCR. During DID sessions, control mice (n = 31) were given access to water only.</p><p><strong>Results: </strong>Relative to sham, iTBS increased alcohol consumption (d=0.72) and preference (d=0.44), however these results were not statistically significant. Female mice receiving iTBS, experienced a significant, large increase in alcohol consumption (p = 0.02, d=1.5). Among water only mice, iTBS (d=-1.01) and cTBS (d=-1.03) significantly reduced BDNF expression.</p><p><strong>Conclusions: </strong>This preclinical model is a feasible method to evaluate rTMS-induced changes in alcohol consumption. This pilot analysis warrants future work evaluating the influence of rTMS parameters and sex on changes in drinking or drug-seeking behaviors.</p>","PeriodicalId":520872,"journal":{"name":"Transcranial magnetic stimulation (2024)","volume":"4 ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12201979/pdf/","citationCount":"0","resultStr":"{\"title\":\"Developing a reverse translational model of low-intensity rTMS in alcohol use disorder: The influence of theta burst stimulation protocols on binge alcohol drinking in mice.\",\"authors\":\"Akriti Dhungana, Daniel M McCalley, Alesha M Heath, Eric P Kraybill, Fatemeh S Mojabi, Jairelisse Morales Morales, Allison R Morningstar, Allyson K Davis, Claudia B Padula, William J Giardino, M Windy McNerney\",\"doi\":\"10.1016/j.transm.2025.100098\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Repetitive Transcranial Magnetic Stimulation (rTMS) is a promising treatment tool for Alcohol Use Disorder (AUD). A challenge facing the field is that the optimal TMS parameters to reduce drinking are unknown. There are now rodent TMS coils which can be adapted to evaluate rTMS-induced changes in alcohol consumption in a rapid, cost-effective manner.</p><p><strong>Objective: </strong>Develop a preclinical model of rTMS in alcohol consuming rodents and collect pilot data to evaluate the influence rTMS parameters (here, theta burst pattern) on change in alcohol consumption and biochemistry.</p><p><strong>Methods: </strong>66 C57BL/6 J mice (32 F) received sham, intermittent Theta Burst Stimulation (iTBS), or continuous Theta Burst Stimulation (cTBS) (14 sessions, 2 sessions/day, low intensity 16mT stimulation). Alcohol consumption and preference were evaluated before and after rTMS using a two-bottle choice, Drinking in the Dark (DID) paradigm. Cortical brain tissue was assayed for BDNF gene expression via qPCR. During DID sessions, control mice (n = 31) were given access to water only.</p><p><strong>Results: </strong>Relative to sham, iTBS increased alcohol consumption (d=0.72) and preference (d=0.44), however these results were not statistically significant. Female mice receiving iTBS, experienced a significant, large increase in alcohol consumption (p = 0.02, d=1.5). Among water only mice, iTBS (d=-1.01) and cTBS (d=-1.03) significantly reduced BDNF expression.</p><p><strong>Conclusions: </strong>This preclinical model is a feasible method to evaluate rTMS-induced changes in alcohol consumption. This pilot analysis warrants future work evaluating the influence of rTMS parameters and sex on changes in drinking or drug-seeking behaviors.</p>\",\"PeriodicalId\":520872,\"journal\":{\"name\":\"Transcranial magnetic stimulation (2024)\",\"volume\":\"4 \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12201979/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Transcranial magnetic stimulation (2024)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1016/j.transm.2025.100098\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/4/18 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Transcranial magnetic stimulation (2024)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.transm.2025.100098","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/4/18 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
Developing a reverse translational model of low-intensity rTMS in alcohol use disorder: The influence of theta burst stimulation protocols on binge alcohol drinking in mice.
Background: Repetitive Transcranial Magnetic Stimulation (rTMS) is a promising treatment tool for Alcohol Use Disorder (AUD). A challenge facing the field is that the optimal TMS parameters to reduce drinking are unknown. There are now rodent TMS coils which can be adapted to evaluate rTMS-induced changes in alcohol consumption in a rapid, cost-effective manner.
Objective: Develop a preclinical model of rTMS in alcohol consuming rodents and collect pilot data to evaluate the influence rTMS parameters (here, theta burst pattern) on change in alcohol consumption and biochemistry.
Methods: 66 C57BL/6 J mice (32 F) received sham, intermittent Theta Burst Stimulation (iTBS), or continuous Theta Burst Stimulation (cTBS) (14 sessions, 2 sessions/day, low intensity 16mT stimulation). Alcohol consumption and preference were evaluated before and after rTMS using a two-bottle choice, Drinking in the Dark (DID) paradigm. Cortical brain tissue was assayed for BDNF gene expression via qPCR. During DID sessions, control mice (n = 31) were given access to water only.
Results: Relative to sham, iTBS increased alcohol consumption (d=0.72) and preference (d=0.44), however these results were not statistically significant. Female mice receiving iTBS, experienced a significant, large increase in alcohol consumption (p = 0.02, d=1.5). Among water only mice, iTBS (d=-1.01) and cTBS (d=-1.03) significantly reduced BDNF expression.
Conclusions: This preclinical model is a feasible method to evaluate rTMS-induced changes in alcohol consumption. This pilot analysis warrants future work evaluating the influence of rTMS parameters and sex on changes in drinking or drug-seeking behaviors.
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