MTT分析的陷阱和其他问题。

IF 2.6 3区医学 Q2 ONCOLOGY

Photodiagnosis and Photodynamic Therapy Pub Date : 2025-08-01 DOI:10.1016/j.pdpdt.2025.104700

David Kessel

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引用次数: 0

摘要

虽然通常被称为“MTT”的比色法确实提供了光损伤对线粒体酶水平影响的指示，但仔细分析表明，所得数据与克隆测定无关。虽然MTT数据可以声称代表“细胞毒性”，但这是一个未定义的术语，似乎与抗肿瘤治疗的目标：肿瘤根除并不一定相关。因此，用这种方法来评估治疗对生存能力的影响是不可靠的。

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Pitfalls and other issues with the MTT assay

While the colorimetric assay commonly referred to as ‘MTT’ does provide an indication of effects of photodamage on mitochondrial enzyme levels, a careful analysis showed that the resulting data were not correlated with a clonogenic assay. While MTT data can be claimed to represent ‘cytotoxicity’, this is an undefined term and appears not to be necessarily correlated with the object of anti-tumor therapy: tumor eradication. Use of this assay for assessing effects of treatment on viability are therefore unreliable.

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来源期刊

Photodiagnosis and Photodynamic Therapy ONCOLOGY-

CiteScore

5.80

自引率

24.20%

发文量

509

审稿时长

50 days

期刊介绍： Photodiagnosis and Photodynamic Therapy is an international journal for the dissemination of scientific knowledge and clinical developments of Photodiagnosis and Photodynamic Therapy in all medical specialties. The journal publishes original articles, review articles, case presentations, "how-to-do-it" articles, Letters to the Editor, short communications and relevant images with short descriptions. All submitted material is subject to a strict peer-review process.