{"title":"MTT分析的陷阱和其他问题。","authors":"David Kessel","doi":"10.1016/j.pdpdt.2025.104700","DOIUrl":null,"url":null,"abstract":"<p><p>While the colorimetric assay commonly referred to as 'MTT' does provide an indication of effects of photodamage on mitochondrial enzyme levels, a careful analysis showed that the resulting data were not correlated with a clonogenic assay. While MTT data can be claimed to represent 'cytotoxicity', this is an undefined term and appears not to be necessarily correlated with the object of anti-tumor therapy: tumor eradication. Use of this assay for assessing effects of treatment on viability are therefore unreliable.</p>","PeriodicalId":94170,"journal":{"name":"Photodiagnosis and photodynamic therapy","volume":" ","pages":"104700"},"PeriodicalIF":2.6000,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Pitfalls and other issues with the MTT assay.\",\"authors\":\"David Kessel\",\"doi\":\"10.1016/j.pdpdt.2025.104700\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>While the colorimetric assay commonly referred to as 'MTT' does provide an indication of effects of photodamage on mitochondrial enzyme levels, a careful analysis showed that the resulting data were not correlated with a clonogenic assay. While MTT data can be claimed to represent 'cytotoxicity', this is an undefined term and appears not to be necessarily correlated with the object of anti-tumor therapy: tumor eradication. Use of this assay for assessing effects of treatment on viability are therefore unreliable.</p>\",\"PeriodicalId\":94170,\"journal\":{\"name\":\"Photodiagnosis and photodynamic therapy\",\"volume\":\" \",\"pages\":\"104700\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2025-06-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Photodiagnosis and photodynamic therapy\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1016/j.pdpdt.2025.104700\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Photodiagnosis and photodynamic therapy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.pdpdt.2025.104700","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
While the colorimetric assay commonly referred to as 'MTT' does provide an indication of effects of photodamage on mitochondrial enzyme levels, a careful analysis showed that the resulting data were not correlated with a clonogenic assay. While MTT data can be claimed to represent 'cytotoxicity', this is an undefined term and appears not to be necessarily correlated with the object of anti-tumor therapy: tumor eradication. Use of this assay for assessing effects of treatment on viability are therefore unreliable.
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