分次剂量和急性剂量对全身照射后大鼠脑的比较影响。

IF 2.4
Manal Mohammed, Azza El-Bahkery, Shereen Shedid
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引用次数: 0

摘要

目的:了解分段和急性全身照射剂量对大脑的不同影响,以及这些不同的剂量计划如何在细胞和分子水平上影响大脑。材料与方法:健康雄性大鼠30只,随机分为5组。组1、组3、组V分别接受0 Gy、6 Gy、8 Gy的全身γ辐照。II组和IV组分别以6 Gy和8 Gy的剂量/4(24小时间隔)照射。照射24小时后,测定总抗氧化能力(TAC)、彗星测定、磷酸化tau (p-tau)蛋白、caspase-3、转化生长因子-β (TGF-β)、微管相关蛋白tau (MAPT)和蛋白激酶- n (PKN)基因表达以及双皮质素(DCX)。结果:本研究表明,根据所采用的给药策略,TAC、DNA损伤、p-tau、caspase-3和DCX的水平不同。与分次剂量相比,急性剂量导致更大的DNA损伤和与神经退行性途径相关的早期分子指标。PKN和MAPT基因表达的变化也被观察到,提示辐射暴露的遗传反应;然而,与相应的分离组相比,急性照射没有显著诱导基因表达变化。TGF-β在(8 Gy/4)辐照下显著升高,而在(8 Gy/4)辐照下显著降低。结论:给药策略可能影响脑抗氧化防御系统对氧化应激、DNA、凋亡通路、神经元功能和结构以及神经发生的反应。TGF-β的结果强调了它在介导大脑对辐射损伤的反应中的信号传导作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative impact of fractionated and acute doses of gamma irradiation on the rats' brain after whole body irradiation.

Purpose: Understanding the distinct impacts of fractionated versus acute doses of whole body irradiation on the brain and how these contrasting dosing schedules affect the brain at a cellular and molecular level.

Materials and methods: Thirty healthy rats (males) were separated into five groups. Groups I, III, and V were irradiated by 0 Gy, 6 Gy, and 8 Gy of whole body γ-irradiation, respectively. Groups II and IV were irradiated by 6 Gy and 8 Gy as fractionated doses/4 (24-hour interval), respectively. Twenty-four hours after irradiation the total antioxidant capacity (TAC), comet assay, phosphorylated tau (p-tau) protein, caspase-3, transforming growth factor-β (TGF-β), microtubule-associated protein tau (MAPT) and protein kinase-N (PKN) gene expressions, and doublecortin (DCX) were measured.

Results: The present study indicated varying levels of TAC, DNA damage, p-tau, caspase-3, and DCX, based on the dosing strategy employed. Acute doses resulted in greater DNA damage and early molecular indicators associated with neurodegenerative pathway compared to fractionated doses. Changes in PKN and MAPT gene expressions were also observed, suggesting genetic responses to radiation exposure; however, the acute irradiation did not significantly induce the gene expression changes compared to the respective fractionated group. In a different manner, TGF-β at (8 Gy) showed significant unique decrease in contrast to the substantial increase after exposure to (8 Gy/4) irradiation.

Conclusions: There is a potential effect of the dosing strategy on brain's antioxidant defense system's response to oxidative stress, DNA, apoptotic pathway, neuronal function and structure, and neurogenesis. TGF-β results underscore its signaling in mediating the brain's response to irradiation-induced damage.

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