二趾树懒（Choloepus diactylus）新型梨质粒的鉴定及系统发育分析。

IF 1.1 4区农林科学 Q3 VETERINARY SCIENCES

Journal of Veterinary Medical Science Pub Date : 2025-08-01 Epub Date: 2025-06-30 DOI:10.1292/jvms.25-0063

Misato Murakami, Ayana Iwasa, Minoru Okamoto, Takahiro Ishizaki, Kazuyuki Suzuki, Haruyuki Hirata

{"title":"二趾树懒（Choloepus diactylus）新型梨质粒的鉴定及系统发育分析。","authors":"Misato Murakami, Ayana Iwasa, Minoru Okamoto, Takahiro Ishizaki, Kazuyuki Suzuki, Haruyuki Hirata","doi":"10.1292/jvms.25-0063","DOIUrl":null,"url":null,"abstract":"This study investigated the pathological and parasitological causes of death in two two-toed sloths (Choloepus didactylus) housed at a zoo in Hokkaido, Japan. Genomic DNA was extracted from the clotted blood of these animals and subsequently examined by nested PCR assays targeting the 18S rRNA and β-tubulin genes. The nucleotide sequences of these genes were determined and a phylogenetic analysis of the 18S rRNA gene was performed. One sample was detected positive in nested PCR. Sequencing analysis revealed that the detected parasite was closely related to the order Piroplasmida and was subsequently designated as Piroplasmida sp. Choloepus didactylus-1 (Piroplasmida sp. CD-1).","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"972-975"},"PeriodicalIF":1.1000,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12344316/pdf/","citationCount":"0","resultStr":"{\"title\":\"Identification and phylogenetic analysis of novel Piroplasmida detected in the two-toed sloth (Choloepus didactylus).\",\"authors\":\"Misato Murakami, Ayana Iwasa, Minoru Okamoto, Takahiro Ishizaki, Kazuyuki Suzuki, Haruyuki Hirata\",\"doi\":\"10.1292/jvms.25-0063\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This study investigated the pathological and parasitological causes of death in two two-toed sloths (Choloepus didactylus) housed at a zoo in Hokkaido, Japan. Genomic DNA was extracted from the clotted blood of these animals and subsequently examined by nested PCR assays targeting the 18S rRNA and β-tubulin genes. The nucleotide sequences of these genes were determined and a phylogenetic analysis of the 18S rRNA gene was performed. One sample was detected positive in nested PCR. Sequencing analysis revealed that the detected parasite was closely related to the order Piroplasmida and was subsequently designated as Piroplasmida sp. Choloepus didactylus-1 (Piroplasmida sp. CD-1).\",\"PeriodicalId\":49959,\"journal\":{\"name\":\"Journal of Veterinary Medical Science\",\"volume\":\" \",\"pages\":\"972-975\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2025-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12344316/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Veterinary Medical Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1292/jvms.25-0063\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/6/30 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Veterinary Medical Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1292/jvms.25-0063","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/6/30 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

摘要

本研究调查了日本北海道一家动物园饲养的两只二趾树懒（Choloepus didactylus）的病理学和寄生虫学死亡原因。从这些动物的凝血中提取基因组DNA，随后通过巢式PCR检测18S rRNA和β-微管蛋白基因。测定了这些基因的核苷酸序列，并对18S rRNA基因进行了系统发育分析。巢式PCR检测阳性者1例。测序结果表明，该寄生虫与螺质虫目亲缘关系密切，鉴定为Piroplasmida sp. Choloepus diactylus -1 （Piroplasmida sp. CD-1）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Identification and phylogenetic analysis of novel Piroplasmida detected in the two-toed sloth (Choloepus didactylus).

This study investigated the pathological and parasitological causes of death in two two-toed sloths (Choloepus didactylus) housed at a zoo in Hokkaido, Japan. Genomic DNA was extracted from the clotted blood of these animals and subsequently examined by nested PCR assays targeting the 18S rRNA and β-tubulin genes. The nucleotide sequences of these genes were determined and a phylogenetic analysis of the 18S rRNA gene was performed. One sample was detected positive in nested PCR. Sequencing analysis revealed that the detected parasite was closely related to the order Piroplasmida and was subsequently designated as Piroplasmida sp. Choloepus didactylus-1 (Piroplasmida sp. CD-1).

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of Veterinary Medical Science 农林科学-兽医学

CiteScore

2.30

自引率

8.30%

发文量

230

审稿时长

9-18 weeks

期刊介绍： JVMS is a peer-reviewed journal and publishes a variety of papers on veterinary science from basic research to applied science and clinical research. JVMS is published monthly and consists of twelve issues per year. Papers are from the areas of anatomy, physiology, pharmacology, toxicology, pathology, immunology, microbiology, virology, parasitology, internal medicine, surgery, clinical pathology, theriogenology, avian disease, public health, ethology, and laboratory animal science. Although JVMS has played a role in publishing the scientific achievements of Japanese researchers and clinicians for many years, it now also accepts papers submitted from all over the world.