Widya Paramita Lokapirnasari, Lilik Maslachah, Amung Logam Saputro, Efi Rokana, Andreas Berny Yulianto, Zulfi Nur Amrina Rosyada, Muhammad Aviv Firdaus, Himatul Ilma Silfia, Ertika Fitri Lisnanti, Zein Ahmad Baihaqi, Tabita Dameria Marbun, Aswin Rafif Khairullah, Muhammad Shakeel
{"title":"禽致病性大肠杆菌攻毒肉鸡中副干酪乳杆菌替代抗生素生长促进剂的效力","authors":"Widya Paramita Lokapirnasari, Lilik Maslachah, Amung Logam Saputro, Efi Rokana, Andreas Berny Yulianto, Zulfi Nur Amrina Rosyada, Muhammad Aviv Firdaus, Himatul Ilma Silfia, Ertika Fitri Lisnanti, Zein Ahmad Baihaqi, Tabita Dameria Marbun, Aswin Rafif Khairullah, Muhammad Shakeel","doi":"10.14202/vetworld.2025.1180-1189","DOIUrl":null,"url":null,"abstract":"<p><strong>Background and aim: </strong>The emergence of antibiotic-resistant bacteria due to the widespread use of antibiotic growth promoters (AGPs) necessitates the exploration of sustainable alternatives in poultry production. This study evaluated the efficacy of <i>Lacticaseibacillus paracasei</i> as a probiotic alternative to AGPs in broilers challenged with avian pathogenic <i>Escherichia coli</i> (APEC), with a focus on growth performance, antioxidant status, lipid metabolism, and hematological profiles.</p><p><strong>Materials and methods: </strong>A total of 80 broiler chickens were randomly allocated into four groups: T0 (uninfected control), T1 (APEC-infected control), T2 (APEC + AGP, 0.1% zinc bacitracin), and T3 (APEC + probiotic, 0.5% <i>L. paracasei</i>). Treatments were administered from day 15 to 35 post-hatch, following a 2-week adaptation period. Performance indicators (feed conversion ratio [FCR], body weight gain [BWG], and feed efficiency [FE]), antioxidant parameters (superoxide dismutase [SOD] and malondialdehyde [MDA]), lipid profiles (cholesterol, HDL, and LDL), and hematological variables were assessed. Data were analyzed using analysis of variance with significance set at p < 0.05.</p><p><strong>Results: </strong>Probiotic supplementation significantly improved FCR, BWG, and FE (p < 0.05), with the T3 group achieving the most favorable outcomes. SOD activity was markedly higher, and MDA levels were reduced in probiotic-treated birds compared with the AGP and infected groups. Moreover, <i>L. paracasei</i> administration resulted in significant reductions in total cholesterol and LDL levels while maintaining moderate HDL concentrations. Hemoglobin and thrombocyte levels were modestly influenced, though overall hematological profiles remained stable across groups.</p><p><strong>Conclusion: </strong><i>L. paracasei</i> supplementation significantly enhanced growth performance, antioxidant defense, and lipid metabolism in broilers challenged with APEC, supporting its application as a viable alternative to AGPs. These findings contribute to sustainable poultry production practices and public health safety by mitigating the reliance on antibiotics.</p>","PeriodicalId":23587,"journal":{"name":"Veterinary World","volume":"18 5","pages":"1180-1189"},"PeriodicalIF":2.0000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12205234/pdf/","citationCount":"0","resultStr":"{\"title\":\"Potency of <i>Lacticaseibacillus paracasei</i> as an alternative to antibiotic growth promoter in broiler chicken challenged with avian pathogenic <i>Escherichia coli</i>.\",\"authors\":\"Widya Paramita Lokapirnasari, Lilik Maslachah, Amung Logam Saputro, Efi Rokana, Andreas Berny Yulianto, Zulfi Nur Amrina Rosyada, Muhammad Aviv Firdaus, Himatul Ilma Silfia, Ertika Fitri Lisnanti, Zein Ahmad Baihaqi, Tabita Dameria Marbun, Aswin Rafif Khairullah, Muhammad Shakeel\",\"doi\":\"10.14202/vetworld.2025.1180-1189\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background and aim: </strong>The emergence of antibiotic-resistant bacteria due to the widespread use of antibiotic growth promoters (AGPs) necessitates the exploration of sustainable alternatives in poultry production. This study evaluated the efficacy of <i>Lacticaseibacillus paracasei</i> as a probiotic alternative to AGPs in broilers challenged with avian pathogenic <i>Escherichia coli</i> (APEC), with a focus on growth performance, antioxidant status, lipid metabolism, and hematological profiles.</p><p><strong>Materials and methods: </strong>A total of 80 broiler chickens were randomly allocated into four groups: T0 (uninfected control), T1 (APEC-infected control), T2 (APEC + AGP, 0.1% zinc bacitracin), and T3 (APEC + probiotic, 0.5% <i>L. paracasei</i>). Treatments were administered from day 15 to 35 post-hatch, following a 2-week adaptation period. Performance indicators (feed conversion ratio [FCR], body weight gain [BWG], and feed efficiency [FE]), antioxidant parameters (superoxide dismutase [SOD] and malondialdehyde [MDA]), lipid profiles (cholesterol, HDL, and LDL), and hematological variables were assessed. Data were analyzed using analysis of variance with significance set at p < 0.05.</p><p><strong>Results: </strong>Probiotic supplementation significantly improved FCR, BWG, and FE (p < 0.05), with the T3 group achieving the most favorable outcomes. SOD activity was markedly higher, and MDA levels were reduced in probiotic-treated birds compared with the AGP and infected groups. Moreover, <i>L. paracasei</i> administration resulted in significant reductions in total cholesterol and LDL levels while maintaining moderate HDL concentrations. Hemoglobin and thrombocyte levels were modestly influenced, though overall hematological profiles remained stable across groups.</p><p><strong>Conclusion: </strong><i>L. paracasei</i> supplementation significantly enhanced growth performance, antioxidant defense, and lipid metabolism in broilers challenged with APEC, supporting its application as a viable alternative to AGPs. These findings contribute to sustainable poultry production practices and public health safety by mitigating the reliance on antibiotics.</p>\",\"PeriodicalId\":23587,\"journal\":{\"name\":\"Veterinary World\",\"volume\":\"18 5\",\"pages\":\"1180-1189\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12205234/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary World\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.14202/vetworld.2025.1180-1189\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/5/17 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary World","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.14202/vetworld.2025.1180-1189","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/5/17 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Potency of Lacticaseibacillus paracasei as an alternative to antibiotic growth promoter in broiler chicken challenged with avian pathogenic Escherichia coli.
Background and aim: The emergence of antibiotic-resistant bacteria due to the widespread use of antibiotic growth promoters (AGPs) necessitates the exploration of sustainable alternatives in poultry production. This study evaluated the efficacy of Lacticaseibacillus paracasei as a probiotic alternative to AGPs in broilers challenged with avian pathogenic Escherichia coli (APEC), with a focus on growth performance, antioxidant status, lipid metabolism, and hematological profiles.
Materials and methods: A total of 80 broiler chickens were randomly allocated into four groups: T0 (uninfected control), T1 (APEC-infected control), T2 (APEC + AGP, 0.1% zinc bacitracin), and T3 (APEC + probiotic, 0.5% L. paracasei). Treatments were administered from day 15 to 35 post-hatch, following a 2-week adaptation period. Performance indicators (feed conversion ratio [FCR], body weight gain [BWG], and feed efficiency [FE]), antioxidant parameters (superoxide dismutase [SOD] and malondialdehyde [MDA]), lipid profiles (cholesterol, HDL, and LDL), and hematological variables were assessed. Data were analyzed using analysis of variance with significance set at p < 0.05.
Results: Probiotic supplementation significantly improved FCR, BWG, and FE (p < 0.05), with the T3 group achieving the most favorable outcomes. SOD activity was markedly higher, and MDA levels were reduced in probiotic-treated birds compared with the AGP and infected groups. Moreover, L. paracasei administration resulted in significant reductions in total cholesterol and LDL levels while maintaining moderate HDL concentrations. Hemoglobin and thrombocyte levels were modestly influenced, though overall hematological profiles remained stable across groups.
Conclusion: L. paracasei supplementation significantly enhanced growth performance, antioxidant defense, and lipid metabolism in broilers challenged with APEC, supporting its application as a viable alternative to AGPs. These findings contribute to sustainable poultry production practices and public health safety by mitigating the reliance on antibiotics.
期刊介绍:
Veterinary World publishes high quality papers focusing on Veterinary and Animal Science. The fields of study are bacteriology, parasitology, pathology, virology, immunology, mycology, public health, biotechnology, meat science, fish diseases, nutrition, gynecology, genetics, wildlife, laboratory animals, animal models of human infections, prion diseases and epidemiology. Studies on zoonotic and emerging infections are highly appreciated. Review articles are highly appreciated. All articles published by Veterinary World are made freely and permanently accessible online. All articles to Veterinary World are posted online immediately as they are ready for publication.