桉树醋对绵羊胃肠道线虫卵的杀卵作用。

IF 2 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Veterinary World Pub Date : 2025-05-01 Epub Date: 2025-05-13 DOI:10.14202/vetworld.2025.1156-1167
Yandra Thais Rocha da Mota, Alexandre Santos Pimenta, Moacir Franco de Oliveira, Ana Karolinne de Alencar França, Andressa Marcelly Silvestre Pereira, Rafael Rodolfo de Melo, Thays Vieira da Costa Monteiro, Maíra Fasciotti, Lúcio César Dantas de Medeiros, Ana Carla Diógenes Suassuna Bezerra
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引用次数: 0

摘要

背景与目的:胃肠道线虫(胃肠道线虫)严重影响全球,特别是热带地区的小型反刍动物生产。由于滥用合成药物而产生的驱虫抗药性,使我们有必要寻找可持续的植物替代品。桉木醋(Eucalyptus wood vinegar, WV)是生物质热解的副产物,具有潜在的驱虫活性。本研究旨在评价桉树WV和桉树木材与马郁兰共热解提取的WV对自然感染绵羊的GINs卵的体外杀卵效果。材料和方法:WV样品通过控制热解制备,并通过顺序真空蒸馏精制。采用5种WV浓度(0.3125% ~ 5% g/100 mL),以噻苯达唑为阳性对照,蒸馏水为阴性对照,进行卵孵化率试验。卵计数、物种鉴定和扫描电子显微镜(SEM)来评估结构变化。采用气相色谱-质谱联用(GC/MS)对WVs的化学成分进行了表征。结果:两种WV均表现出显著的杀卵活性,桉树WV在1.25%的抑制率达到97%,而富含马郁兰的WV在5%的抑制率达到100%。GC/MS分析显示,马郁兰制剂中含有酚类化合物、糠醛、百里香酚和桉树醇,后两种化合物为马郁兰制剂所独有。扫描电镜显微照片证实了处理过的卵子的形态变形,包括对称性和膜完整性的丧失。生物活性成分,特别是百里香酚、桉树醇和糠醛之间的协同相互作用被认为是增强杀卵活性的机制。结论:尤加利桉(Eucalyptus WV),特别是通过共热解富集的尤加利桉(o.m majorana),对绵羊体内的GINs具有较强的杀卵作用。这些发现支持了wv作为生态友好的驱虫替代品在小型反刍动物寄生虫综合管理策略中的潜在应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Ovicidal effect of eucalyptus wood vinegar on gastrointestinal nematodes' eggs from sheep.

Background and aim: Gastrointestinal nematodes (GINs) significantly impair small ruminant production globally, particularly in tropical regions. Anthelmintic resistance due to the indiscriminate use of synthetic drugs has necessitated the search for sustainable, plant-based alternatives. Eucalyptus wood vinegar (WV), a by-product of biomass pyrolysis, possesses bioactive compounds with potential anthelmintic activity. This study aimed to assess the in vitro ovicidal efficacy of eucalyptus WV and WV derived from co-pyrolysis of eucalyptus wood with Origanum majorana (marjoram) against eggs of GINs from naturally infected sheep.

Materials and methods: WV samples were produced through controlled pyrolysis and refined through sequential vacuum distillation. Egg hatchability tests were performed using five WV concentrations (0.3125%-5% g/100 mL), with thiabendazole as a positive control and distilled water as a negative control. Egg counts, species identification, and scanning electron microscopy (SEM) were conducted to evaluate structural changes. The chemical compositions of the WVs were characterized using gas chromatography-mass spectrometry (GC/MS).

Results: Both WVs exhibited significant ovicidal activity, with eucalyptus WV achieving 97% inhibition at 1.25%, and the marjoram-enriched WV reaching 100% inhibition at 5%. GC/MS analysis revealed the presence of phenolic compounds, furfural, thymol, and eucalyptol, the latter two being exclusive to the marjoram formulation. SEM micrographs confirmed morphological deformations in treated eggs, including loss of symmetry and membrane integrity. The synergistic interaction among bioactive components, particularly thymol, eucalyptol, and furfural, is proposed as the mechanism enhancing ovicidal activity.

Conclusion: Eucalyptus WV, particularly when enriched with O. majorana through co-pyrolysis, exhibits potent ovicidal effects against GINs in sheep. These findings support the potential use of WVs as eco-friendly anthelmintic alternatives in integrated parasite management strategies for small ruminants.

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来源期刊
Veterinary World
Veterinary World Multiple-
CiteScore
3.60
自引率
12.50%
发文量
317
审稿时长
16 weeks
期刊介绍: Veterinary World publishes high quality papers focusing on Veterinary and Animal Science. The fields of study are bacteriology, parasitology, pathology, virology, immunology, mycology, public health, biotechnology, meat science, fish diseases, nutrition, gynecology, genetics, wildlife, laboratory animals, animal models of human infections, prion diseases and epidemiology. Studies on zoonotic and emerging infections are highly appreciated. Review articles are highly appreciated. All articles published by Veterinary World are made freely and permanently accessible online. All articles to Veterinary World are posted online immediately as they are ready for publication.
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