褪黑素对猪体外卵母细胞成熟和胚胎发育的影响。

IF 2 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Veterinary World Pub Date : 2025-05-01 Epub Date: 2025-05-21 DOI:10.14202/vetworld.2025.1234-1241
Laura Andrea Blancas-Alvarez, Alma Lilia Alvarez-Guerrero, Alicia Alcantar-Rodriguez, Alfredo Medrano
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引用次数: 0

摘要

背景和目的:猪体外受精(IVF)效率仍然不理想,部分原因是卵母细胞成熟和胚胎发育过程中的氧化应激。褪黑素(Melatonin, MLT)是一种内源性抗氧化剂,被认为是生殖培养系统的有益补充。本研究旨在探讨不同浓度的MLT对猪卵母细胞体外成熟及胚胎发育的影响。材料和方法:卵巢取自当地某屠宰场的青春期前后备母猪。将1142个卵母细胞卵母细胞复合物(COC)分为四组,分别在0、1、3、5 μM MLT下体外成熟44小时。卵母细胞成熟度采用乙酰-卵黄蛋白染色和台锥蓝染色进行评估。随后,1312 COC使用标准化精子浓度进行体外受精,然后在北卡罗莱纳州立大学-23培养基中添加相应的MLT浓度培养胚胎8天。胚胎发育分为卵裂期、桑葚胚期和早期囊胚期。资料采用Tukey事后检验方差分析(p < 0.05)。结果:虽然添加1.0 μM MLT的中期卵母细胞成熟率最高(45.9%±5.70%),囊胚形成率最高(26.9%±9.57%),但在卵母细胞成熟、COC活力和胚胎发育阶段方面,不同处理间无显著差异(p < 0.05)。结论:在猪卵母细胞成熟和胚胎培养过程中添加0-5 μM MLT对体外成熟率和胚胎发育结局没有显著影响。MLT在猪卵母细胞和胚胎体外培养过程中的作用机制有待进一步研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of melatonin on in vitro oocyte maturation and embryo development in pigs.

Background and aim: In vitro fertilization (IVF) efficiency in pigs remains suboptimal, partly due to oxidative stress during oocyte maturation and embryo development. Melatonin (MLT), an endogenous antioxidant, has been proposed as a beneficial supplement in reproductive culture systems. This study aimed to evaluate the effects of different concentrations of MLT on in vitro porcine oocyte maturation and subsequent embryo development.

Materials and methods: Ovaries were obtained from prepubertal gilts at a local slaughterhouse. A total of 1142 cumulus-oocyte-complexes (COC) were allocated into four groups and matured in vitro with 0, 1, 3, or 5 μM MLT for 44 h. Oocyte maturation was assessed using aceto-orcein staining and viability with trypan blue staining. Subsequently, 1312 COC underwent IVF using a standardized sperm concentration, followed by embryo culture in North Carolina State University-23 medium supplemented with corresponding MLT concentrations for 8 days. Embryo development was classified according to cleavage, morula, and early blastocyst stages. Data were analyzed using analysis of variance with Tukey's post hoc test (p < 0.05).

Results: Although supplementation with 1.0 μM MLT resulted in the highest metaphase II oocyte maturation rate (45.9% ± 5.70%) and blastocyst formation (26.9% ± 9.57%), no significant differences were observed among treatments in either oocyte maturation, COC viability, or embryo development stages (p > 0.05).

Conclusion: Supplementation with 0-5 μM MLT during oocyte maturation and embryo culture did not significantly enhance in vitro maturation rates or embryonic developmental outcomes in pigs. Further investigations are warranted to optimize MLT concentrations and elucidate its mechanistic role during porcine oocyte and embryo in vitro culture.

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来源期刊
Veterinary World
Veterinary World Multiple-
CiteScore
3.60
自引率
12.50%
发文量
317
审稿时长
16 weeks
期刊介绍: Veterinary World publishes high quality papers focusing on Veterinary and Animal Science. The fields of study are bacteriology, parasitology, pathology, virology, immunology, mycology, public health, biotechnology, meat science, fish diseases, nutrition, gynecology, genetics, wildlife, laboratory animals, animal models of human infections, prion diseases and epidemiology. Studies on zoonotic and emerging infections are highly appreciated. Review articles are highly appreciated. All articles published by Veterinary World are made freely and permanently accessible online. All articles to Veterinary World are posted online immediately as they are ready for publication.
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