快速检测传染性造血坏死病毒（IHNV）的现场可用横向流动免疫层析试验。

IF 2.2 3区农林科学 Q2 FISHERIES

Journal of fish diseases Pub Date : 2025-06-30 DOI:10.1111/jfd.70014

Choon-Sup Kim, Kyoung-Hui Kong, Sung-Hun Lee, Wi-Sik Kim

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引用次数: 0

摘要

建立了虹鳟（Oncorhynchus mykiss）传染性造血坏死病毒（IHNV，基因组JRt-N和JRt-S）的横向流动免疫层析快速检测方法。该试验利用单克隆抗体（mAb） IHNV-3，它既是胶体金-mAb偶联物中的检测抗体，也是测试线上的捕获抗体。JRt-N和JRt-S 6株IHNV分离株的检测限在105.05 ~ 105.8 TCID50/100 μL之间，与其它鱼类病毒无交叉反应。对81条感染ihnv的虹鳟鱼和102条未感染虹鳟鱼的脾脏样本进行现场验证，结果显示特异性为100%，敏感性为97.5%，总体诊断效率为98.9%。这些结果表明，LFIA条带是一种可靠的工具，可在养殖设施虹鳟鱼中快速（10分钟内）现场检测IHNV。

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Field-Usable Lateral Flow Immunochromatographic Assay for Rapid Detection of Infectious Haematopoietic Necrosis Virus (IHNV).

A lateral flow immunochromatographic assay (LFIA) was developed for the rapid detection of infectious haematopoietic necrosis virus (IHNV, genogroups JRt-N and JRt-S) in rainbow trout (Oncorhynchus mykiss). The assay utilises the monoclonal antibody (mAb) IHNV-3, which serves as both the detector antibody in a colloidal gold-mAb conjugate and the capture antibody on the test line. The LFIA strip demonstrated a detection limit ranging from 10^5.05 to 10^5.8 TCID₅₀/100 μL for six IHNV isolates (genogroups JRt-N and JRt-S) and showed no cross-reactivity with other fish viruses. Field validation using spleen samples from 81 IHNV-infected and 102 non-infected rainbow trout revealed a specificity of 100%, a sensitivity of 97.5%, and an overall diagnostic effectiveness of 98.9%. These results highlight the LFIA strip as a reliable tool for the rapid (within 10 min) on-site detection of IHNV in rainbow trout at aquaculture facilities.

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来源期刊

Journal of fish diseases 农林科学-海洋与淡水生物学

CiteScore

4.60

自引率

12.00%

发文量

170

审稿时长

6 months

期刊介绍： Journal of Fish Diseases enjoys an international reputation as the medium for the exchange of information on original research into all aspects of disease in both wild and cultured fish and shellfish. Areas of interest regularly covered by the journal include: -host-pathogen relationships- studies of fish pathogens- pathophysiology- diagnostic methods- therapy- epidemiology- descriptions of new diseases