hdpsc衍生的分泌体增强牙源性分化和抑制炎症：再生牙髓治疗的潜在无细胞方法。

IF 3.6 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of endodontics Pub Date : 2025-10-01 DOI:10.1016/j.joen.2025.06.016

Ji-Soo Kim DDS, MSD , Manfei Qiu DDS, MSD, PhD , Kkot-Byeol Bae DDS, MSD, PhD , Ji-Hyun Jang DDS, MSD, PhD , Won-Jae Kim DDS, MSD, PhD , Jeong-Tae Koh DDS, MSD, PhD , Yun-Chan Hwang DDS, MSD, PhD , Bin-Na Lee DDS, MSD, PhD

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引用次数: 0

摘要

简介：分泌组是由干细胞分泌的生物活性分子，含有生长因子、抗炎剂和参与组织修复的蛋白质。本研究旨在评价人牙髓干细胞（hDPSCs）衍生分泌组的抗炎和成牙作用。方法：采用逐级过滤、离心分离的方法分离分泌组。进行基因本体分析以表征分泌组的组成。通过伤口愈合试验评估分泌组对细胞迁移的影响。采用定量实时聚合酶链反应（qRT-PCR）和western blotting检测炎症标志物和牙源分化标志物，评估抗炎和牙源性作用。在脂多糖（LPS）诱导的炎症条件下，采用碱性磷酸酶（ALP）染色评估矿化。数据采用单因素方差分析（ANOVA）和Tukey事后检验（p < 0.05）进行统计学分析。结果：基因本体分析确定了分泌组的关键功能成分。伤口愈合实验表明，100 μg/mL分泌组显著增强了hDPC的迁移。分泌组治疗显著减弱lps诱导的炎症标志物上调（p < 0.05），同时上调成牙细胞分化标志物并促进矿化（p < 0.05）。结论：hdpsc衍生的分泌组具有抗炎和成牙作用，可能由MAPK信号通路介导。这些发现表明，分泌体可能作为再生牙髓治疗的一种新的无细胞策略，为牙本质治疗和牙本质-牙髓再生提供了治疗潜力。

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hDPSC-derived Secretomes Enhance Odontogenic Differentiation and Suppress Inflammation: A Potential Cell-free Approach for Regenerative Endodontics

Introduction

Secretomes are bioactive molecules secreted by stem cells that contain growth factors, anti-inflammatory agents, and proteins involved in tissue repair. This study aimed to evaluate the anti-inflammatory and odontogenic effects of human dental pulp stem cells (hDPSCs)-derived secretomes.

Methods

Secretomes were isolated from hDPSCs through stepwise filtration and centrifugation. Gene ontology analysis was performed to characterize the secretome composition. The effects of secretomes on cell migration were assessed via wound healing assay. Anti-inflammatory and odontogenic effects were evaluated using quantitative real-time polymerase chain reaction and western blotting for inflammatory markers and odontogenic differentiation markers. Alkaline phosphatase staining was performed to assess mineralization under lipopolysaccharide-induced inflammatory conditions. Data were statistically analyzed using one-way analysis of variance and Tukey's posthoc test (P < .05 was considered significant).

Results

Gene ontology analysis confirmed key functional components of the secretome. The wound healing assay demonstrated that 100 μg/mL secretome treatment significantly enhanced human dental pulp cells migration. Secretome treatment significantly attenuated lipopolysaccharide-induced upregulation of inflammatory markers (P < .05) while upregulating odontoblastic differentiation markers and promoting mineralization (P < .05).

Conclusion

hDPSC-derived secretomes exhibit anti-inflammatory and odontogenic effects, potentially mediated by MAPK signaling pathways. These findings suggest that secretomes may serve as a novel cell-free strategy for regenerative endodontics, offering therapeutic potential for vital pulp therapy and dentin-pulp regeneration.

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来源期刊

Journal of endodontics 医学-牙科与口腔外科

CiteScore

8.80

自引率

9.50%

发文量

224

审稿时长

42 days

期刊介绍： The Journal of Endodontics, the official journal of the American Association of Endodontists, publishes scientific articles, case reports and comparison studies evaluating materials and methods of pulp conservation and endodontic treatment. Endodontists and general dentists can learn about new concepts in root canal treatment and the latest advances in techniques and instrumentation in the one journal that helps them keep pace with rapid changes in this field.