纳米强化生物载体:氧化铁修饰壳聚糖和海藻酸钙微球,用于提高气泡柱生物反应器的发酵效率和可重复使用性。

IF 2.1 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
David Abutu, B O Aderemi, A O Ameh, Hafizuddin Wan Yussof, Augustine Agi
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引用次数: 0

摘要

壳聚糖球(CB)和海藻酸钙球(CAB)被广泛用于固定化酿酒酵母的发酵,但其机械强度低,表面积有限,导致乙醇产量下降。为了克服这些限制,将氧化铁(Fe₂O₃)纳米颗粒掺入CB和CAB中,以提高机械强度和表面积。在35℃、空气流速为0.01 L/min、pH为4.0的半间歇发酵条件下,将改性载体在气泡柱生物反应器中固定化酿酒酵母15 h。Fe₂O₃纳米颗粒的掺入显著提高了CB和CAB的破裂力,分别从2±0.05 N增加到8±0.5 N和2±0.05 N增加到9±0.07 N。CB的表面积从18±0.3 m2/g增加到48±0.2 m2/g, CAB的表面积从2±0.2 m2/g增加到50±0.1 m2/g,导致细胞吸附从1.13 × 10⁸增加到1.10 × 10⁹细胞/mL,乙醇收率从37±0.28%提高到45±1.23%。未改性的CB和CAB在重复使用5次后出现明显的断裂,而改性后的珠粒保持了结构的完整性和活性,证明了它们对酵母固定和重复使用的耐久性。该方法为提高乙醇生产中的发酵效率和载体稳定性提供了一种有前途的策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Nano-enhanced biocarriers: ferric oxide-modified chitosan and calcium alginate beads for improved fermentation efficiency and reusability in a bubble column bioreactor.

Chitosan beads (CB) and calcium alginate beads (CAB) are widely used for immobilizing Saccharomyces cerevisiae in fermentation, but their low mechanical strength and limited surface area reduce ethanol yield. To overcome these limitations, ferric oxide (Fe₂O₃) nanoparticles were incorporated into CB and CAB to enhance both mechanical strength and surface area. The modified carriers were employed for Saccharomyces cerevisiae immobilization in a bubble column bioreactor under semi-batch fermentation conditions at 35 °C, an air flow rate of 0.01 L/min, and pH 4.0 for 15 h. The Fe₂O₃ nanoparticles incorporation significantly improved the rupture forces of CB and CAB, increasing from 2 ± 0.05 N to 8 ± 0.5 N and 2 ± 0.05 N to 9 ± 0.07 N, respectively. The surface area of CB increased from 18 ± 0.3 m2/g to 48 ± 0.2 m2/g, while CAB increased from 2 ± 0.2 m2/g to 50 ± 0.1 m2/g, leading to enhanced cell adsorption from 1.13 × 10⁸ to 1.10 × 10⁹ cells/mL Consequently, ethanol yield improved from 37 ± 0.28% to 45 ± 1.23%. Unlike unmodified CB and CAB, which exhibited significant rupture after five reuse cycles, the modified beads retained their structural integrity and activity, demonstrating their durability for yeast immobilization and reuse. This approach offers a promising strategy for enhancing fermentation efficiency and carrier stability in ethanol production.

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来源期刊
Biotechnology Letters
Biotechnology Letters 工程技术-生物工程与应用微生物
CiteScore
5.90
自引率
3.70%
发文量
108
审稿时长
1.2 months
期刊介绍: Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.
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